Derivatization, an Applicable Asset for Conventional HPLC Systems without MS Detection in Food and Miscellaneous Analysis

Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

“…The derivatization process increases the sensitivity of detection by improving the optical or chromatographic properties of the molecule [32]. Several advantages are associated with pre‐column derivatization over post‐column derivatization [23]. Pre‐column derivatization does not require special separation columns or detector treatment, making it relatively straightforward and adaptable to multiple analytical samples.…”

“…In the analysis of BCAAs, there are two common approaches, one is to detect the natural form of the AAs, and the other is to assist the analysis by adding chemical groups and producing the derivative forms of AAs. Derivatization is a chemical reaction that utilizes a derivatizing reagent to react with specific groups in the target compound, producing a new substance [23]. Derivatization of BCAAs can increase their UV absorbance and FL as they lack UV absorption and FL signals, but also the ionization [24].…”

“…, Thr (4), His (5), Cyn-mono-CBI (6), Glu (7), Gly (8), Tyr (9), Ala (10), Asp (11), Tau (12), Ala-Gln (13), Val (14), Met (15), nVal (16), Trp (17), Ile (18), Leu (19), Phe (20), Arg ( 21), Cyn-bis-CBI (22) and Lys (23). [81] with permission.…”