Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride

Heimbecher, Susan; Lee, Yung Chi; Tabibi, S. Esmail; Yalkowsky, Samuel H.

doi:10.1016/s0378-4347(96)00452-5

Cited by 6 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BAs are also known as precursors of carcinogenic nitrosamines and are not reduced by high-temperature treatment. [7][8][9][10] Determination of BAs is a difficult task because of their diverse chemical structure and usually present at low levels in a complex matrix. 11,12 Several analytical methods for the determination of BAs in foods have been developed.…”

Section: Introductionmentioning

confidence: 99%

A simple method for benzoyl chloride derivatization of biogenic amines for high performance liquid chromatography

et al. 2014

View full text Add to dashboard Cite

A simple, economical and reproducible benzoylation procedure was developed for the derivatization of biogenic amines (BAs) prior to high-performance liquid chromatography determination. The significant factors affecting biogenic amine benzoylation yield were optimized by central composite design. The derivatized BAs were extracted from basic aqueous solutions by 2-undecanol for direct HPLC injection. The obtained optimal conditions significantly decreased reagent consumption, uncertainty and time of analysis. The optimized method was used for further determination of BAs in real samples of nonalcoholic beers. The method showed good linearity (correlation coefficients > 0.997) and good recoveries (from 87.3 to 96.3%). The repeatability and reproducibility of the method were >4.6% and >6.7% respectively. Moreover, the detection limits of BAs were calculated between 0.02 and 0.09 mg ml À1 in non-alcoholic beers samples.

show abstract

Section: Introductionmentioning

confidence: 99%

A simple method for benzoyl chloride derivatization of biogenic amines for high performance liquid chromatography

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Increased polyamine levels are implicated to play a causative rather than associative role in cancer [20]. As a result, analogs of polyamines which inhibit the metabolism of polyamines are exploited for cancer treatment and prevention [21]. Therefore, assays of polyamines in blood have been used to detect cancer progression and to determine the success of therapy.…”

Section: Introductionmentioning

confidence: 99%

Quantification of polyamines in human erythrocytes using a new near‐infrared cyanine 1‐(ϵ‐succinimidyl‐hexanoate)‐ 1′‐methyl‐3,3,3′,3′‐tetramethyl‐ indocarbocyanine‐5,5′‐disulfonate potassium with CE‐LIF detection

Zhang

et al. 2007

Electrophoresis

View full text Add to dashboard Cite

A novel near-infrared (NIR) cyanine 1-(epsilon-succinimidyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium (MeCy5-OSu) has been developed in our laboratory. Simultaneous determination of MeCy5-OSu-derivatized polyamines spermine (Spm), spermidine (Spd), cadaverine (Cad), and putrescine (Put) based on the separation by CE combined with diode LIF detection has been accomplished. The highest derivatization efficiency was achieved in 0.2 mol/L borate buffer (pH 8.8) for 20 min at 25 degrees C. Polyamine derivatives were separated within 14 min in the phosphate running buffer (pH 3) containing 50 mmol/L phosphoric acid, 40 mmol/L SDS, and 35% methanol v/v. Linearity of response was obtained in the range of 10-200 nmol/L. The detection limits (S/N = 3) for Spm, Spd, Cad, and Put were 0.8, 1, 3, and 2 nmol/L, respectively. The proposed method has been successfully applied to the analysis of polyamines in erythrocytes of two healthy persons and one cancer patient. Average recoveries for erythrocyte samples were 93.6-106% and coefficients of variation ranged from 1.8 to 5.4%. The analysis of polyamines in erythrocytes can be used for studying the relationship between their changes and the carcinogenesis process involved in erythrocytes.

show abstract

Selective fluorometric detection of polyamines using micellar electrokinetic chromatography with laser-induced fluorescence detection

Paproski

Roy

Lucy

2002

Journal of Chromatography A

View full text Add to dashboard Cite

Derivatization and high-performance liquid chromatographic analysis of pentaazapentacosane pentahydrochloride

Cited by 6 publications

References 13 publications

A simple method for benzoyl chloride derivatization of biogenic amines for high performance liquid chromatography

A simple method for benzoyl chloride derivatization of biogenic amines for high performance liquid chromatography

Quantification of polyamines in human erythrocytes using a new near‐infrared cyanine 1‐(ϵ‐succinimidyl‐hexanoate)‐ 1′‐methyl‐3,3,3′,3′‐tetramethyl‐ indocarbocyanine‐5,5′‐disulfonate potassium with CE‐LIF detection

Selective fluorometric detection of polyamines using micellar electrokinetic chromatography with laser-induced fluorescence detection

Contact Info

Product

Resources

About