Deriving causal relationships in resting-state functional connectivity using SSFO-based optogenetic fMRI

Han, Xu; Cramer, Samuel; Zhang, Nanyin

doi:10.1088/1741-2552/ac9d66

Cited by 5 publications

(2 citation statements)

References 53 publications

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“…To minimize animals’ stress and motion during awake fMRI imaging, rats were habituated to the restraining and fMRI scanning environment for 7 days (15, 30, 45, 60, 60, 60 and 60 min/day, respectively). Detailed acclimation procedures can be found in our previous publications (Tu et al, 2021; Han et al, 2022). All fMRI data were acquired on a 7T Bruker BioSpec 70/30 scanner interfaced with ParaVision 6.0.1 (Bruker, Billerica, MA).…”

Section: Methodsmentioning

confidence: 99%

Exploring memory-related network via dorsal hippocampus suppression

Han,

Cramer,

Chan

et al. 2024

Preprint

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Memory is a complex brain process that requires coordinated activities in a large-scale brain network. However, the relationship between coordinated brain network activities and memory-related behavior is not well understood. In this study, we investigated this issue by suppressing the activity in the dorsal hippocampus (dHP) using chemogenetics and measuring the corresponding changes in brain-wide resting-state functional connectivity (RSFC) and memory behavior in awake rats. We identified an extended brain network contributing to the performance in a spatial-memory related task. Our results were cross-validated using two different chemogenetic actuators, clozapine (CLZ) and clozapine-N-oxide (CNO). This study provides a brain network interpretation of memory performance, indicating that memory is associated with coordinated brain-wide neural activities.Significance StatementSuccessful memory processes require coordinated activity in a large-scale brain network, extending beyond a few key, well-known brain regions like the hippocampus. However, the specific brain regions involved and how they orchestrate their activity that is pertinent to memory processing remain unclear. Our study, using a chemogenetics-rsfMRI- behavior approach in awake rats, elucidates a comprehensive framework of the extended memory-associated network. This knowledge offers a broader interpretation of memory processes, enhancing our understanding of the neural mechanisms behind memory function, particularly from a network perspective.

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Section: Methodsmentioning

confidence: 99%

Exploring memory-related network via dorsal hippocampus suppression

Han,

Cramer,

Chan

et al. 2024

Preprint

Self Cite

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“…No handling of rats precedes acclimation training. All rats imaged by studies in Group 1b are male (512/529) or unspecified (17/529) ( Han et al. 2022 ) and most (21/24) belong to the LE strain.…”

Section: Awake Fmri In Ratsmentioning

confidence: 99%

Where do we stand on fMRI in awake mice?

Mandino,

Vujic,

Grandjean

et al. 2023

Cerebral Cortex

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Imaging awake animals is quickly gaining traction in neuroscience as it offers a means to eliminate the confounding effects of anesthesia, difficulties of inter-species translation (when humans are typically imaged while awake), and the inability to investigate the full range of brain and behavioral states in unconscious animals. In this systematic review, we focus on the development of awake mouse blood oxygen level dependent functional magnetic resonance imaging (fMRI). Mice are widely used in research due to their fast-breeding cycle, genetic malleability, and low cost. Functional MRI yields whole-brain coverage and can be performed on both humans and animal models making it an ideal modality for comparing study findings across species. We provide an analysis of 30 articles (years 2011–2022) identified through a systematic literature search. Our conclusions include that head-posts are favorable, acclimation training for 10–14 d is likely ample under certain conditions, stress has been poorly characterized, and more standardization is needed to accelerate progress. For context, an overview of awake rat fMRI studies is also included. We make recommendations that will benefit a wide range of neuroscience applications.

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Neuromodulation in Small AnimalfMRI

Hsu,

Shih

2024

Magnetic Resonance Imaging

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The integration of functional magnetic resonance imaging (fMRI) with advanced neuroscience technologies in experimental small animal models offers a unique path to interrogate the causal relationships between regional brain activity and brain‐wide network measures—a goal challenging to accomplish in human subjects. This review traces the historical development of the neuromodulation techniques commonly used in rodents, such as electrical deep brain stimulation, optogenetics, and chemogenetics, and focuses on their application with fMRI. We discuss their advantageousness roles in uncovering the signaling architecture within the brain and the methodological considerations necessary when conducting these experiments. By presenting several rodent‐based case studies, we aim to demonstrate the potential of the multimodal neuromodulation approach in shedding light on neurovascular coupling, the neural basis of brain network functions, and their connections to behaviors. Key findings highlight the cell‐type and circuit‐specific modulation of brain‐wide activity patterns and their behavioral correlates. We also discuss several future directions and feature the use of mediation and moderation analytical models beyond the intuitive evoked response mapping, to better leverage the rich information available in fMRI data with neuromodulation. Using fMRI alongside neuromodulation techniques provide insights into the mesoscopic (relating to the intermediate scale between single neurons and large‐scale brain networks) and macroscopic fMRI measures that correlate with specific neuronal events. This integration bridges the gap between different scales of neuroscience research, facilitating the exploration and testing of novel therapeutic strategies aimed at altering network‐mediated behaviors. In conclusion, the combination of fMRI with neuromodulation techniques provides crucial insights into mesoscopic and macroscopic brain dynamics, advancing our understanding of brain function in health and disease.Evidence Level1Technical EfficacyStage 1

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Deriving causal relationships in resting-state functional connectivity using SSFO-based optogenetic fMRI

Cited by 5 publications

References 53 publications

Exploring memory-related network via dorsal hippocampus suppression

Exploring memory-related network via dorsal hippocampus suppression

Where do we stand on fMRI in awake mice?

Neuromodulation in Small AnimalfMRI

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