Water hyacinth (Eichhornia crassipes) has become a threat to many aquatic environments worldwide. This aquatic weed presents a rapid reproductive capacity and outcompetes other aquatic plant species, forming dense, free-floating mats, which in many instances completely cover fresh-water surfaces. The infestation leads to several serious environmental (including ecological and socioeconomic) impacts that are hazardous to aquatic systems, disables human uses of surface waters, and affects hydraulic infrastructures (e.g., waterways and pumping stations). Our study explores the use of remote sensing tools to monitor and categorize the spread of water hyacinth, aiming at new insights into the detection, observation, and mapping of this invasive plant using vegetation indices and water indices calculated from multispectral data from satellite Sentinel-2, such as normalized difference vegetation index and normalized difference water index. The approach uses spatiotemporal information and has the potential to contribute to inform planners and decision-makers that are concerned with managing the plant by applying integrated measures. The case study deals with a small water course located in the downstream part of the Mondego river valley in Portugal, a country where water hyacinth is widely spread and constitutes a major problem, mainly in the irrigated agricultural areas of the Tagus, Sado, Mondego, and Lis rivers' basins.