Deriving stable tropical gynoecious inbred lines of slicing cucumber from American pickling cucumber using MABB

Behera, Tusar Kanti; Boopalakrishnan, G.; Jat, Gograj Singh; Munshi, A. D.; Choudhary, Harshawardhan; Ravindran, Amarnath; Kumari, Shilpi; Kumari, Rajni

doi:10.1007/s13580-021-00392-5

Cited by 9 publications

(3 citation statements)

References 37 publications

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“…Cucumber (Cucumis sativus L.) is a plant of the family Cucurbitaceae which is produced worldwide in open fields and protected agriculture [1]. Cucumber fruit is widely consumed for its taste and freshness around the world, in addition, its nutritional contribution and nutraceutical properties have positive impacts on health, especially in people with diabetes, hypertension, cardiovascular, and Alzheimer's disease [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria Improve Growth and Fruit Quality of Cucumber under Greenhouse Conditions

Zapata-Sifuentes

Hernández-Montiel²,

Sáenz‐Mata

et al. 2022

Plants

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Cucumber fruit is rich in fiber, carbohydrates, protein, magnesium, iron, vitamin B, vitamin C, flavonoids, phenolic compounds, and antioxidants. Agrochemical-based production of cucumber has tripled yields; however, excessive synthetic fertilization has caused problems in the accumulation of salts in the soil and has increased production costs. The objective of this study was to evaluate the effect of three strains of plant growth-promoting rhizobacteria (PGPR) on cucumber fruit growth and quality under greenhouse conditions. The rhizobacteria Pseudomonas paralactis (KBendo6p7), Sinorhizobium meliloti (KBecto9p6), and Acinetobacter radioresistens (KBendo3p1) was adjusted to 1 × 108 CFU mL−1. The results indicated that the inoculation with PGPR improved plant height, stem diameter, root length, secondary roots, biomass, fruit size, fruit diameter, and yield, as well as nutraceutical quality and antioxidant capacity, significantly increasing the response of plants inoculated with A.radioresistens and S.meliloti in comparison to the control. In sum, our findings showed the potential functions of the use of beneficial bacteria such as PGPR for crop production to reduce costs, decrease pollution, and achieve world food safety and security.

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Section: Introductionmentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria Improve Growth and Fruit Quality of Cucumber under Greenhouse Conditions

Zapata-Sifuentes

Hernández-Montiel²,

Sáenz‐Mata

et al. 2022

Plants

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“…Genetic variation in segregated population of cucumber plays a significant role in cucumber improvement programs. Behera et al (2022) and Yang et al (2022) were able to select superior lines of cucumber with the desired traits and identify specific genes responsible for these traits using F2 population. Genetic variation analysis gives information on the population's genetic variety and genetic structure, as well as a platform for selecting superior genotypes to create new cultivars in crop improvement breeding programs (Verma et al 2019).…”

Section: Phenotypic Traits Variation In F2 Population Of Cucumbermentioning

confidence: 99%

Phenotypic variability evaluation and genetic variation in F2 intraspecific hybrids of cucumber (Cucumis sativus L.) using retrotransposon-based markers

SETIAWAN,

ZAHIDAH,

KALTSUM

et al. 2023

Biodiversitas

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Abstract. Setiawan AB, Zahidah QA, Kaltsum DN, Purwantoro A. 2023. Phenotypic variability evaluation and genetic variation in F2 intraspecific hybrids of cucumber (Cucumis sativus L.) using retrotransposon-based markers. Biodiversitas 24: 2596-2604. Cucumber (Cucumis sativus L.) has a narrow genetic base compared to other Cucumis species. The genetic base of the cucumber can be improved by crossing two distantly related cultivars from different geographical locations. This study aimed to evaluate phenotypic variability and investigate genetic variation of F2 cucumber population using two retrotransposon-based markers, namely Inter Retrotransposon Amplified Polymorphism (IRAP)andInter-SINE Amplified Polymorphism(ISAP). Two parental lines and the selected 59 genotypes from F2 population were subjected to PCR analysis for DNA profiling. The phenotypic variation of F2 genotypes revealed significant diversity in fruit weight, fruit diameter, fruit radian, pedicel length, fruit length, and fruit ratio (length-diameter). The genetic variation of the F2 hybrids was successfully detected and discriminated by IRAP and ISAP markers. Both IRAP and ISAP markers showed high heterozygosity ranged from 0.4021 to 0.4997, with an average of 0.4649 and moderate polymorphic information content ranged from 0.3489 to 0.3929 with an average of 0.3648. Cluster analysis showed that the F2 population and parental lines were grouped into two major clusters with a similarity coefficient ranged from 0.67 to 0.96. These findings imply that the F2 population is highly segregated and can be utilized to create new cultivars of cucumber.

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“…Pusa Pickling Cucumber-8 (DG-8) was the second gynoecious parthenocarpic genotype which is a speciality genotype suitable for pickling and cultivation under protected conditions. The non-parthenocarpic genotype, IMPU-1, was developed through introgression of F locus-determining gynoecy into a commercially cultivated elite monoecious genotype, Pusa Uday, through marker assisted breeding (MABC) (Behera et al, 2022). All the genotypes under investigation were gynoecious in nature, thus enabling a precise analysis of important phytohormones and determining the role of different CK, AUX and GA biosynthesis and degradation genes in inducing parthenocarpy in different groups of cucumber.…”

Section: Plant Materials and Growing Conditionsmentioning

confidence: 99%

Cross-talk between the cytokinin, auxin, and gibberellin regulatory networks in determining parthenocarpy in cucumber

et al. 2022

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Cucumber is a model plant for studying parthenocarpy with abundant slicing- and pickling-type germplasm. This study was undertaken to understand the role of the important cytokines (CKs), auxin (AUX) and gibberellin (GA) biosynthesis and degradation genes for the induction of parthenocarpy in slicing and pickling germplasm. Two genotypes of gynoecious parthenocarpic cucumber, PPC-6 and DG-8, along with an MABC-derived gynoecious non-parthenocarpic line, IMPU-1, were evaluated in this study. The slicing and pickling cucumber genotypes PPC-6 and DG-8 were strongly parthenocarpic in nature and set fruit normally without pollination. Endogenous auxin and gibberellin were significantly higher in parthenocarpic than non-parthenocarpic genotypes, whereas the concentration of cytokinins varied among the genotypes at different developmental stages. However, the exogenous application of Zeatin and IAA + Zeatin was effective in inducing parthenocarpic fruit in IMPU-1. Expression analysis with important CK, AUX, and GA biosynthesis-related genes was conducted in IMPU-1, PPC-6, and DG-8. The expression of the CK synthase, IPT, IPT3, PaO, LOG1, LOG2, CYP735A1, and CYP735A2 was up-regulated in the parthenocarpic genotypes. Among the transcription factor response regulators (RRs), positive regulation of CSRR8/9b, CSRR8/9d, CSRR8/9e, and CSRR16/17 and negative feedback of the CK signalling genes, such as CsRR3/4a, CsRR3/4b, CsRR8/9a, and CsRR8/9c, were recorded in the parthenocarpic lines. Homeostasis between cytokinin biosynthesis and degradation genes such as CK oxidases (CKXs) and CK dehydrogenase resulted in a non-significant difference in the endogenous CK concentration in the parthenocarpic and non-parthenocarpic genotypes. In addition, up-regulation of the key auxin-inducing proteins and GA biosynthesis genes indicated their crucial role in the parthenocarpic fruit set of cucumber. This study establishes the critical role of the CKs, AUX, and GA regulatory networks and their cross-talk in determining parthenocarpy in slicing and pickling cucumber genotypes.

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Deriving stable tropical gynoecious inbred lines of slicing cucumber from American pickling cucumber using MABB

Cited by 9 publications

References 37 publications

Plant Growth-Promoting Rhizobacteria Improve Growth and Fruit Quality of Cucumber under Greenhouse Conditions

Plant Growth-Promoting Rhizobacteria Improve Growth and Fruit Quality of Cucumber under Greenhouse Conditions

Phenotypic variability evaluation and genetic variation in F2 intraspecific hybrids of cucumber (Cucumis sativus L.) using retrotransposon-based markers

Cross-talk between the cytokinin, auxin, and gibberellin regulatory networks in determining parthenocarpy in cucumber

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