Dermal scatter reduction in human skin: A method using controlled application of glycerol

Fox, Matthew A.; Diven, Dayna G.; Sra, K.K.; Boretsky, Adam; Poonawalla, Tasneem; Readinger, Allison; Motamedi, Massoud; McNichols, Roger J.

doi:10.1002/lsm.20767

Cited by 23 publications

(20 citation statements)

References 13 publications

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“…However, at low concentrations OCAs do not provide sufficient optical clearing, while at high concentrations they can induce edema, chemical burn, partial necrosis and scarring [45,65,108]. To develop an efficient and safe way of breaking the integrity of the epidermis stratum corneum and accelerating the penetration of OCA into the dermis, various physical methods [56,118,[130][131][132][133][134][135][136][137][138][139][140], chemical enhancers of permeability [114,118,[141][142][143][144][145][146][147], and their combinations [118,148,149] have been proposed.…”

Section: Immersion Clearingmentioning

confidence: 99%

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Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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Section: Immersion Clearingmentioning

confidence: 99%

“…In Ref. [139] the authors used slides with cyanoacrylate applied to the skin with moderate pressure, kept nearly 3 minutes, and then were left for 2 minutes more. When tearing the slide off the outer layer of the epidermis was removed.…”

Section: Immersion Clearingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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“…tissues, tissue phantoms) to microscopic (molecular) level and at both in vitro and in vivo studies . Various physical methods, such as light irradiation , light fractional ablation , ultrasound application , sandpaper grinding , microneedle rolling and mechanical compression , or chemical‐penetration enhancers were used to enhance the delivery of OCAs into tissue, so as to improve the tissue optical‐clearing efficacy. This paper focuses on an overview of recent progress in the development and applications of TOC technique, which includes mechanisms of TOC, methods for enhancing TOC efficacy and applications of TOC for tissue optical slicing.…”

Section: Introductionmentioning

confidence: 99%

Recent progress in tissue optical clearing

Zhu

Larin

Luo

et al. 2013

Laser & Photonics Reviews

335

269

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Tissue optical clearing technique provides a prospective solution for the application of advanced optical methods in life sciences. This paper gives a review of recent developments in tissue optical clearing techniques. The physical, molecular and physiological mechanisms of tissue optical clearing are overviewed and discussed. Various methods for enhancing penetration of optical-clearing agents into tissue, such as physical methods, chemical-penetration enhancers and combination of physical and chemical methods are introduced. Combining the tissue optical clearing technique with advanced microscopy image or labeling technique, applications for 3D microstructure of whole tissues such as brain and central nervous system with unprecedented resolution are demonstrated. Moreover, the difference in diffusion and/or clearing ability of selected agents in healthy versus pathological tissues can provide a highly sensitive indicator of the tissue health/pathology condition. Finally, recent advances in optical clearing of soft or hard tissue for in vivo imaging and phototherapy are introduced.

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“…The aqueous solutions of glycerol are immersion liquids widely used as OCAs due to their efficiency, availability, and biocompatibility [1][2][3][4][5][6][7][8]. Unfortunately, in spite of the wide use of glycerol for controlling the tissue optical parameters, there is no unambiguous answer to the question about the optimal concentration of the glycerol solution.…”

Section: Introductionmentioning

confidence: 99%

Ex vivo investigation of glycerol diffusion in skin tissue

Genin

Tuchina

Sadeq

et al. 2016

JBPE

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Abstract. The change of optical parameters of rat skin ex vivo under the action of aqueous 30%, 50%, 60%, 70%, 85%, and 100% glycerol solutions has been experimentally studied. The analysis of kinetics of the change of the studied parameters has allowed the estimate of the diffusion coefficient dependence upon the concentration of glycerol in the skin and the efficiency of optical clearing for each of the glycerol solutions. The results can find application in the development of new methods of noninvasive diagnostics and therapy of skin diseases.

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Dermal scatter reduction in human skin: A method using controlled application of glycerol

Abstract: Topically applied glycerol increased light penetration of in vivo corneal-stripped skin. This minimally invasive approach to temporary dermal scatter reduction has the potential to improve the efficacy of light-based diagnostic or therapeutic devices.

Cited by 23 publications

References 13 publications

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Recent progress in tissue optical clearing

Ex vivo investigation of glycerol diffusion in skin tissue

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