Desarrollo de Complejos de Cobre con Actividad Antitumoral: Síntesis y Caracterización de un nuevo Complejo de Cobre-Terpiridina

Abstract

ResumenEl objetivo de este trabajo es la síntesis y caracterización de nuevos complejos de Cobre(II) y 2,2':6'2''-terpiridina y la evaluación de su actividad antitumoral, como parte de una investigación de complejos de Cu(II) con actividad citotóxica. La estructura del nuevo complejo [Cu(terpy)(H 2 O)(SO 4 )]·2H 2 O se caracterizó por espectroscopía infrarroja en estado sólido y en solución acuosa, espectroscopía electrónica, conductimetría y voltametría cíclica. Se realizaron ensayos de lipofilia y de actividad antiproliferativa en células HeLa. Se determinó que el compuesto obtenido presenta un átomo de Cobre(II) coordinado con una molécula de terpiridina y un anión sulfato. En solución acuosa, el anión sulfato es sustituido por moléculas de agua formándose el ion [Cu(terpy)(H 2 O) 3 ] 2+ . Los estudios de lipofilia muestran un coeficiente de reparto n-octanol/agua de 1,3, que es similar a los valores encontrados para otros compuestos con actividad biológica. El compuesto presenta actividad antiproliferativa de 25 % de inhibición a 25 µM. AbstractThe aim of this research is the synthesis and characterization of new Copper(II)-2,2':6'2''terpyridine complexes, as well as the evaluation of their antitumor activity, as a part of a project on copper complexes with cytotoxic activity. The new complex [Cu(terpy)(H 2 O)(SO 4 )]·2H 2 O was characterized by infrared spectroscopy in solid state and aqueous solution, electronic spectra, conductimetry and cyclic voltammetry. Besides, its lipophilicity and antriproliferative activity in HeLa cells cultures were assessed. The results show that there is a copper atom in the complex that is coordinated to a terpyridine molecule and a sulphate anion. Aqueous solution behavior indicates that the sulphate anion is replaced by three water molecules being [Cu(terpy)(H 2 O) 3 ] 2+ the resulting ion. Lipophilicity studies show a partition coefficient between n-octanol and water of 1,3, similar to other compounds presenting biological activity. The antiproliferative activity was 25 % at 25 µM.

Zinc Oxide and Copper Chitosan Composite Films with Antimicrobial Activity

“…In the FTIR spectrum of the copper nanoparticles (Supplementary Materials, Figure S2) bands at 2918 cm −1 , 1557 cm −1 , 1291 cm −1 , 994 cm −1 , 856 cm −1 , 758 cm −1 , 666 cm −1 , 638 cm −1 , 628 cm −1 , and 608 cm −1 were observed. Some studies report that bands recorded from 700 cm −1 to 400 cm −1 are assigned to Cu-O vibrations, which would confirm the presence of Cu 2 O [31] whereas Ceja-Romero et al reported Cu-O bands at 572 cm −1 [32]. Despite the low copper concentration used, our study, a shoulder at 947 cm −1 , and bands at 895 cm −1 , 875 cm −1 , 715 cm −1 and 659 cm −1 were observed that coincides with the chitosan and Cu film (Figure 1).…”