Description and complete mitochondrial genome of Atkinsoniella zizhongi sp. nov. (Hemiptera: Cicadellidae: Cicadellinae) from China and its phylogenetic implications

Abstract

A new species, Atkinsoniella zizhongi sp. nov. of the subfamily Cicadellinae, was described and illustrated from China. The new species is similar to A. nigrominiatula (Jacobi, 1944), A. limba Kuoh, 1991, A. dormana Li, 1992, A. peaka Yang, Meng et Li, 2017, and A. divaricata Yang, Meng et Li, 2017. But the characteristics of aedeagus and pygofer process can be used to distinguish them easily. The complete mitochondrial genome of the paratype was sequenced and assembled. The mitogenome of A. zizhongi sp. nov. was 16,483 bp in length, with an A+T content of 75.9%, containing 37 typical genes and a control region (CR). The gene order was consistent with the inferred insect ancestral mitochondrial genome. All of the PCGs were determined to have the typical stop codon TAA or TAG, while COX2 and ND5 ended with incomplete termination codons T and TA, respectively. In addition, phylogenetic trees were reconstructed based on PCGs and rRNAs using both the maximum likelihood (ML) and Bayesian inference (BI) methods. The results showed that the intergeneric and interspecific relationships within the subfamily Cicadellinae were completely consistent in all of the phylogenetic trees, except that the different interspecific relationships within the genus Bothrogonia were detected in the ML analysis based on the amino acid sequences. This study enriches the species diversity of Cicadellinae and further promotes research on its phylogeny.

Comparative analysis of codon usage patterns and phylogenetic implications of five mitochondrial genomes of the genus Japanagallia Ishihara, 1955 (Hemiptera, Cicadellidae, Megophthalminae)

“…The GC content was generally lower than the AT content, that is, the genes were rich in AT. This result supports previous studies on the mitochondrial genome of Atkinsoniella zizhongi ( Jiang et al, 2022 ), Centrotoscelus davidi ( Li et al, 2022 ), Bhatia longiradiata ( Lu, Huang & Deng, 2023 ). In addition, AT richness was also reported in mitochondrial genomes of soft scales ( Lu, Huang & Deng, 2023 ) supporting this study.…”

Three new species of Atkinsoniella (Arthropoda, Insecta, Hemiptera, Cicadellidae, Cicadellinae) from China, with an updated checklist to the known species worldwide

“…Yang et al (2017) conducted a systematic morphological study of 88 Atkinsoniella species from China, including 33 new species, two Chinese new records, 12 new synonyms, and proposed Curvufacies Kuoh, 1993 as a new synonym of Atkinsoniella . Subsequently, 2 new species from China and Pakistan were described ( Naveed and Zhang 2018 ; Jiang et al 2022 ).…”

“…To date, 99 valid species were described worldwide, of which 89 species occurred in China, and a few were scattered in Bay of Bengal, Bhutan, India, Indonesia, Kingdom of Bhutan, Laos, Malay Islands, Malaysia, Myanmar, Nepal, Pakistan, Philippines, Thailand, and Vietnam ( Feng and Zhang 2015 ; Yang et al 2017 ; Naveed and Zhang 2018 ; Jiang et al 2022 ). In this study, the description, male genitalia and habitus photos of three new species, A.…”