Description and interpretation of structural changes in canine kidneys after preservation with a hyperosmolar solution and reperfusion

Abstract

The effects of hypothermic preservation of canine kidneys for 24 hours by the use of the Sacks solution, and additional autogenous reperfusion were studied by means of submicroscopic morphological methods. While preservation with the Sacks solution was to ensure normal organization of the lipoprotein structures of the basement and cell membranes, in case of additional reperfusion, even of short duration, a substantial or complete loss of anisotropy of the cytomembranes was demonstrable. The present report is the first to be published in the literature on polarization microscopic studies concerned with the effects of kidney preservation and of reperfusion.