Description and validation of a new set of PCR markers predictive of avian pathogenic Escherichia coli virulence

Lucas, Camille; Delannoy, Sabine; Schouler, Catherine; Souillard, Rozenn; Devendec, Laëtitia Le; Lucas, Pierrick; Keïta, Alassane; Fach, Patrick; Puterflam, Julie; Bougeard, Stéphanie; Kempf, Isabelle

doi:10.1016/j.vetmic.2022.109530

Cited by 3 publications

(1 citation statement)

References 36 publications

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“…Traditional biochemical reactions and ELISA methods for APEC identification are costly and time-consuming. Therefore, the PCR method has been found to be a fast and effective common technique for APEC detection ( Wang et al, 2014 ; Lucas et al, 2022 ).…”

Section: Diagnosis Prevention and Control Of Avian Pathogenic ...mentioning

confidence: 99%

Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenic Escherichia coli (APEC)

Afayibo

Zhang

et al. 2022

Front. Microbiol.

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Although most Escherichia coli (E. coli) strains are commensal and abundant, certain pathogenic strains cause severe diseases from gastroenteritis to extraintestinal infections. Extraintestinal pathogenic E. coli (ExPEC) contains newborn meningitis E. coli (NMEC), uropathogenic E. coli (UPEC), avian pathogenic E. coli (APEC), and septicemic E. coli (SEPEC) based on their original host and clinical symptom. APEC is a heterogeneous group derived from human ExPEC. APEC causes severe respiratory and systemic diseases in a variety of avians, threatening the poultry industries, food security, and avian welfare worldwide. APEC has many serotypes, and it is a widespread pathogenic bacterium in poultry. In addition, ExPEC strains share significant genetic similarities and similar pathogenic mechanisms, indicating that APEC potentially serves as a reservoir of virulence and resistance genes for human ExPEC, and the virulence and resistance genes can be transferred to humans through food animals. Due to economic losses, drug resistance, and zoonotic potential, APEC has attracted heightened awareness. Various virulence factors and resistance genes involved in APEC pathogenesis and drug resistance have been identified. Here, we review the characteristics, epidemiology, pathogenic mechanism zoonotic potential, and drug resistance of APEC, and summarize the current status of diagnosis, alternative control measures, and vaccine development, which may help to have a better understanding of the pathogenesis and resistance of APEC, thereby reducing economic losses and preventing the spread of multidrug-resistant APEC to humans.

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Section: Diagnosis Prevention and Control Of Avian Pathogenic ...mentioning

confidence: 99%

Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenic Escherichia coli (APEC)

Afayibo

Zhang

et al. 2022

Front. Microbiol.

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The in vitro Antimicrobial Effects of Hydrolysable and Condensed Tannin Extracts on Escherichia coli Isolated from Pathological Samples of Deceased Poultry

Ćilerdžić,

Radalj,

Ilić

et al. 2024

Acta Veterinaria

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Colibacillosis, caused by avian pathogenic Escherichia coli (APEC), is one of the most prevalent and economically damaging bacterial diseases affecting poultry globally. Managing colibacillosis is difficult and frequently ineffective because APEC strains have developed widespread resistance to antibiotics, and the strict regulations and public concerns towards using antimicrobial agents in poultry further complicate the situation. This study aimed to evaluate the antibacterial properties of hydrolysable (sweet chestnut extract) and condensed (quebracho extract) tannins on Escherichia coli (E. coli) isolates from poultry, exploring their potential as antibiotic alternatives in managing colibacillosis. E. coli was isolated from the internal organs of deceased poultry across 18 farms, including layers, broilers, and broiler breeders. Each isolate was assessed for the presence of APEC strain predictors (virulence genes iutA, hlyF, iss, iroN, and ompT), antimicrobial resistance to 14 antibiotics using the disc diffusion method, and the presence of resistance genes for specific antibiotics (ampicillin, gentamicin, tetracycline, and quinolones). Out of 43 isolates, 27 (62.8%) were classified as APEC, 30 (69.8%) showed resistance to three or more antibiotic classes, and 32 (74.4%) carried at least one AMR gene. The minimum inhibitory concentrations (MICs) for the hydrolysable tannins from sweet chestnut extract (Castanea sativa Mill.) (SwCh) ranged from 0.5 to 3 mg/mL, while for the condensed tannins from quebracho extract (Schinopsis lorentzii) (Que), the MICs ranged from 1.5 to 4.5 mg/mL. The results indicate that both hydrolysable and condensed tannins possess significant in vitro antimicrobial activity against APEC, offering a potentially valuable alternative for controlling colibacillosis in the poultry industry.

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Fluoroquinolone resistance determinants in carbapenem-resistant Escherichia coli isolated from urine clinical samples in Thailand

Boueroy,

Chopjitt,

Hatrongjit

et al. 2023

PeerJ

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Background Escherichia coli is the most common cause of urinary tract infections and has fluoroquinolone (FQ)-resistant strains, which are a worldwide concern. Objectives To characterize FQ-resistant determinants among 103 carbapenem-resistant E. coli (CREc) urinary isolates using WGS. Methods Antimicrobial susceptibility, biofilm formation, and short-read sequencing were applied to these isolates. Complete genome sequencing of five CREcs was conducted using short- and long-read platforms. Results ST410 (50.49%) was the predominant ST, followed by ST405 (12.62%) and ST361 (11.65%). Clermont phylogroup C (54.37%) was the most frequent. The genes NDM-5 (74.76%) and CTX-M-15 (71.84%) were the most identified. Most CREcs were resistant to ciprofloxacin (97.09%) and levofloxacin (94.17%), whereas their resistance rate to nitrofurantoin was 33.98%. Frequently, the gene aac(6′)-Ib (57.28%) was found and the coexistence of aac(6′)-Ib and blaCTX-M-15 was the most widely predominant. All isolates carried the gyrA mutants of S83L and D87N. In 12.62% of the isolates, the coexistence was detected of gyrA, gyrB, parC, and parE mutations. Furthermore, the five urinary CREc-complete genomes revealed that blaNDM-5 or blaNDM-3 were located on two plasmid Inc types, comprising IncFI (60%, 3/5) and IncFI/IncQ (40%, 2/5). In addition, both plasmid types carried other resistance genes, such as blaOXA-1, blaCTX-M-15, blaTEM-1B, and aac(6′)-Ib. Notably, the IncFI plasmid in one isolate carried three copies of the blaNDM-5 gene. Conclusions This study showed FQ-resistant determinants in urinary CREc isolates that could be a warning sign to adopt efficient strategies or new control policies to prevent further spread and to help in monitoring this microorganism.

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Description and validation of a new set of PCR markers predictive of avian pathogenic Escherichia coli virulence

Cited by 3 publications

References 36 publications

Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenic Escherichia coli (APEC)

Characteristics, pathogenic mechanism, zoonotic potential, drug resistance, and prevention of avian pathogenic Escherichia coli (APEC)

The in vitro Antimicrobial Effects of Hydrolysable and Condensed Tannin Extracts on Escherichia coli Isolated from Pathological Samples of Deceased Poultry

Fluoroquinolone resistance determinants in carbapenem-resistant Escherichia coli isolated from urine clinical samples in Thailand

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