Description of Ovariectomy Protocol in Mice

Souza, Vanessa Rodrigues de; Mendes, Erasmo Garcia; Casaro, Mateus B.; Antiorio, Ana Tada Fonseca Brasil; Oliveira, Fernando A.; Ferreira, Caroline M.

doi:10.1007/978-1-4939-8994-2_29

Cited by 82 publications

(61 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We therefore hypothesized that JYP could produce comparable efficacy in alleviating menopause-related psychiatric disorders compared to estrogen therapy in association with differential mechanisms at peripheral reproductive organs and the brain. Ovariectomized (OVX) animals are often used as a model of estrogen deprivation as it is well-validated to represent most estrogen deprivation-associated clinical features in the adult human ( 23 ). We have modified this model by exposing the OVX mice to chronic unpredictable mild stress (CUMS) to facilitate the development of mood disorder-like behavior and cognitive impairment ( 16 , 17 ).…”

Section: Introductionmentioning

confidence: 99%

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome

et al. 2020

View full text Add to dashboard Cite

Jie-Yu Pill (JYP) is a proprietary herbal medicine initially developed to treat menstrual mood disorders. This study sought to determine whether JYP could alleviate menopausal psychiatric symptoms in ovariectomized (OVX) mice, an animal model of estrogen deprivation, exposed to chronic unpredictable mild stress (CUMS) and the underlying mechanisms in comparison with estrogen therapy. The OVX+CUMS mice were treated with 0.3 mg/kg estradiol (E 2), 2.5 g/kg or 5 g/kg JYP for 36 days, and tested in multiple behavioral paradigms. Serum, uterus, and brain tissues were collected for the measurement of hypothalamus-pituitary-ovarian axis (HPO) and hypothalamus-pituitary-adrenal (HPA) axis hormones, γ-aminobutyric acid (GABA), glutamate, neurotrophins, and estrogen receptors. JYP and E 2 had comparable efficacy in reducing anxiety-and depression-like behavior and cognitive impairment of the OVX+CUMS mice. E 2 strikingly increased ratio of uterus to body weight of the OVX+CUMS mice, but JYP did not. Both agents suppressed HPO-axis upstream hormones, inhibited HPA-axis hyperactivity by reinstating hypothalamic GABA, restored hippocampal and prefrontal glutamate contents and its receptor expression in the OVX+CUMS mice. While JYP and E 2 protected against decreases in hippocampal and prefrontal neurotrophins and estrogen receptors of the OVX+CUMS mice, unlike E 2 , JYP had no significant effects on these biomarkers in the uterus. These results suggest that JYP has comparable efficacy in ameliorating mood disorder-like behavior and cognitive impairment induced by a combination of estrogen deprivation and chronic stress in association with certain differential Zhou et al. JYP for Menopause uterus-brain mechanisms compared to estrogen therapy. JYP may be a potential therapy for menopause-associated psychiatric disorders.

show abstract

Section: Introductionmentioning

confidence: 99%

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome

et al. 2020

View full text Add to dashboard Cite

show abstract

“…For this purpose, control mice, which produce ovarian hormone normally, are needed. Eight‐week‐old C57BL/6 mice (female) were used for OVX, as previously reported 28,29 . Mice anaesthetized by inhalation anaesthesia had bilateral ovaries removed through the dorsal approach.…”

Section: Methodsmentioning

confidence: 99%

Sphingosylphosphorylcholine blocks ovariectomy‐induced bone loss by suppressing Ca²⁺/calmodulin‐mediated osteoclast differentiation

Lee

Cho

Kim

et al. 2020

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Thus, the surgical manipulation of rodents offers an effective alternative to study the gonadal effects in multiple systems, including cardiovascular [ 144 ]. Therefore, in order to mimic the hormonal status present in post-menopausal females and to understand sex differences in CVD, female rodents are usually subjected to ovariectomy (OVX) [ 145 ]. The OVX procedure consists of a clinical menopause induction by the excision of the ovaries and a subsequent depletion of ovarian hormones.…”

Section: Mouse and Cell Experimental Models To Address Sex Differementioning

confidence: 99%

Approaching Sex Differences in Cardiovascular Non-Coding RNA Research

Jusić

Salgado-Somoza

Paes

et al. 2020

IJMS

View full text Add to dashboard Cite

Cardiovascular disease (CVD) is the biggest cause of sickness and mortality worldwide in both males and females. Clinical statistics demonstrate clear sex differences in risk, prevalence, mortality rates, and response to treatment for different entities of CVD. The reason for this remains poorly understood. Non-coding RNAs (ncRNAs) are emerging as key mediators and biomarkers of CVD. Similarly, current knowledge on differential regulation, expression, and pathology-associated function of ncRNAs between sexes is minimal. Here, we provide a state-of-the-art overview of what is known on sex differences in ncRNA research in CVD as well as discussing the contributing biological factors to this sex dimorphism including genetic and epigenetic factors and sex hormone regulation of transcription. We then focus on the experimental models of CVD and their use in translational ncRNA research in the cardiovascular field. In particular, we want to highlight the importance of considering sex of the cellular and pre-clinical models in clinical studies in ncRNA research and to carefully consider the appropriate experimental models most applicable to human patient populations. Moreover, we aim to identify sex-specific targets for treatment and diagnosis for the biggest socioeconomic health problem globally.

show abstract

Description of Ovariectomy Protocol in Mice

Cited by 82 publications

References 7 publications

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome

Sphingosylphosphorylcholine blocks ovariectomy‐induced bone loss by suppressing Ca²⁺/calmodulin‐mediated osteoclast differentiation

Approaching Sex Differences in Cardiovascular Non-Coding RNA Research

Contact Info

Product

Resources

About

Description of Ovariectomy Protocol in Mice

Cited by 82 publications

References 7 publications

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome

Jie-Yu Pill, A Proprietary Herbal Medicine, Ameliorates Mood Disorder-Like Behavior and Cognitive Impairment in Estrogen-Deprived Mice Exposed to Chronic Unpredictable Mild Stress: Implication for a Potential Therapy of Menopause Syndrome

Sphingosylphosphorylcholine blocks ovariectomy‐induced bone loss by suppressing Ca2+/calmodulin‐mediated osteoclast differentiation

Approaching Sex Differences in Cardiovascular Non-Coding RNA Research

Contact Info

Product

Resources

About

Sphingosylphosphorylcholine blocks ovariectomy‐induced bone loss by suppressing Ca²⁺/calmodulin‐mediated osteoclast differentiation