Descriptive study of the mitogenome of the diamondback squid ( Thysanoteuthis rhombus Troschel, 1857) and the evolution of mitogenome arrangement in oceanic squids

Abstract

The diamondback squid, Thysanoteuthis rhombus, is the only currently accepted species within the family Thysanoteuthidae. In this study, the mitogenome of T. rhombus was sequenced using the Illumina NovaSeq 6000 platform. Features of the mitogenome were detected, and phylogenetic relationships among the Cephalopoda were reconstructed. Our results indicated that the mitogenome of T. rhombus had six duplicated genes and long non‐coding regions. The gene arrangement of T. rhombus was consistent with that of species belonging to Ommastrephidae. Moreover, the most gene rearrangement patterns were found in Oegopsida and Octopoda, whereas only one pattern was detected in Sepioidea. The mitogenome of Myopsida was highly rearranged among the four taxa. The phylogenetic relationship of T. rhombus + (((Dosidicus gigas + Sthenoteuthis oualaniensis) + Ommastrephes bartramii) + (Illex argentines + Todarodes pacificus)) was highly supported by both mitochondrial gene rearrangement analyses and all the phylogenetic analyses. Our study indicated that both gene sequences and arrangements may provide more information on the phylogeny and evolution of diverse mitogenomes in Cephalopoda and contribute to resolving contentious interclass relationships in the future.

Gene Rearrangements in the Mitochondrial Genome of Gonatopsis borealis and Onychoteuthis compacta Reveal Their Phylogenetic Implications for Oegopsida

“…According to previous studies (Bernt et al 2013a), four gene blocks were identi ed in Bilateria and de ned as conserved block. In Tang's study (Tang et al 2021), four complete conserved blocks were found in some cephalopods, namely ( 1) nad2-cox1-cox2-trnD-atp8-atp6; (2) nad4l-nad4 -trnH-nad5; (3) rrnS-trnV-rrnL-trnL1-trnL2-nad1; (4) nad6-cytb-trnS. In the present study, in addition to Ancistrocheirus sp, Lampadioteuthis megaleia, Stigmatoteuthis arcturi and Dosidicus gigas did not conform to the nad2-cox1-cox2-atp8, the other Oegopsida suborder species all conform to this conserved block.…”

Characterization of the Complete Mitochondrial Genome of Sea Duck Mergus serrator and Comparison with other Anseriformes Species

“…Total genomic DNA was extracted from muscle tissue using the Easy Pure genomic DNA kit (Trans Gen Biotech Co, Beijing, China). The original sequence data (15.65 G) was deposited in NCBI's Sequence Read Archive (SRA; accession: SRR13516389), and complete mitogenome sequencing (15.62 G) was performed on an Illumina NovaSeq6000 platform (Novogene Bioinformatics Technology Co. Ltd., Tianjin, China) (Tang et al, 2021). The mitogenome was assembled using the program NOVO Plasty 3.7 (Dierckxsens et al, 2017) and then adjusted manually.…”

Distribution and geographic differences in stable isotope ratios of diamond squid Thysanoteuthis rhombus (Cephalopoda: Thysanoteuthidae) in Japanese waters

“…Diamond squid Thysanoteuthis rhombus (Troschel, 1857), the only member of the family Thysanoteuthidae (Tang et al, 2021), grows to at least 100 cm in dorsal mantle length (DML) and 24 kg in both sexes (Roper & Jerez, 2010). The growth rate is rapid (Miyahara, Ota, Goto, & Gorie, 2006).…”