Desiccation induced changes in osmolytes production and the antioxidative defence in the cyanobacterium Anabaena sp. PCC 7120

Singh, Priyanka; Tiwari, Anupam; Singh, Satnam; Asthana, Ravi Kumar

doi:10.1007/s12298-012-0145-3

Cited by 9 publications

(2 citation statements)

References 40 publications

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“…In desiccated state, Chl a and carotenoid contents increased after 1 h of incubation under desiccation. The oxygen-evolving activity declined in desiccated cyanobacterial biomass while rehydration led to instant recovery, indicating that cells protect the photosynthetic machinery against desiccation [257]. As desiccation leads to oxidative stress, an enhanced level of carotenoids most likely facilitates quenching of ROS.…”

Section: Changing the Cultivation Conditions For Optimizing The Prmentioning

confidence: 99%

Plastids of Marine Phytoplankton Produce Bioactive Pigments and Lipids

et al. 2013

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Phytoplankton is acknowledged to be a very diverse source of bioactive molecules. These compounds play physiological roles that allow cells to deal with changes of the environmental constrains. For example, the diversity of light harvesting pigments allows efficient photosynthesis at different depths in the seawater column. Identically, lipid composition of cell membranes can vary according to environmental factors. This, together with the heterogenous evolutionary origin of taxa, makes the chemical diversity of phytoplankton compounds much larger than in terrestrial plants. This contribution is dedicated to pigments and lipids synthesized within or from plastids/photosynthetic membranes. It starts with a short review of cyanobacteria and microalgae phylogeny. Then the bioactivity of pigments and lipids (anti-oxidant, anti-inflammatory, anti-mutagenic, anti-cancer, anti-obesity, anti-allergic activities, and cardio- neuro-, hepato- and photoprotective effects), alone or in combination, is detailed. To increase the cellular production of bioactive compounds, specific culture conditions may be applied (e.g., high light intensity, nitrogen starvation). Regardless of the progress made in blue biotechnologies, the production of bioactive compounds is still limited. However, some examples of large scale production are given, and perspectives are suggested in the final section.

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Section: Changing the Cultivation Conditions For Optimizing The Prmentioning

confidence: 99%

Plastids of Marine Phytoplankton Produce Bioactive Pigments and Lipids

et al. 2013

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“…Extreme desiccation for longer period can be tolerated by many prokaryotes (Potts 1994). Drought and salt stress induced trehalose and sucrose accumulation in cyanobacteria (Hershkowitz et al 1991;Sakamoto et al 2009); in addition to sucrose, trehalose, antioxidative enzymes (catalase, peroxidase, and SOD), and proline level also fluctuated in Anabaena 7120 under desiccation ( Singh et al 2013). Intracellular concentrations of total amino acids including proline varied in desiccated cells of Tolypothrix scytonemoides (Rajendran et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

Singh

Tiwari

Singh

et al. 2013

Physiol Mol Biol Plants

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Drought is the most important abiotic stress, challenging sustainable agriculture globally. For desiccation being the multigenic trait, a combination of identified genes from the appropriate organism may render crop tolerant to the water stress. Among the compatible solutes, proline plays multifaceted role in counteracting such stress. The genes encoding proline biosynthesizing enzymes, glutamate 5-kinase (G5K), and pyrroline-5-carboxylate reductase (P5CR) from the low-desiccation-tolerant cyanobacterium Anabaena sp. PCC 7120, were cloned and overexpressed in Escherichia coli BL21(DE3) individually. The recombinant E. coli cells harboring G5K, failed to exhibit enhanced desiccation tolerance relative to those with P5CR that showed increased growth/survival over the wild type. This may be ascribed to the overexpression of the reductase gene. Multiple sequence alignment showed P5CR to be conserved in all the organisms. We hypothesize that P5CR gene from high-desiccation-tolerant cyanobacteria may be adopted as the candidate for making transgenic N 2 -fixing cyanobacterium for paddy fields and/or crop development in future.

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Augmenting the Abiotic Stress Tolerance in Plants Through Microbial Association

Singh

Roychoudhury

2021

Microbial Metatranscriptomics Belowground

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Desiccation induced changes in osmolytes production and the antioxidative defence in the cyanobacterium Anabaena sp. PCC 7120

Cited by 9 publications

References 40 publications

Plastids of Marine Phytoplankton Produce Bioactive Pigments and Lipids

Plastids of Marine Phytoplankton Produce Bioactive Pigments and Lipids

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

Augmenting the Abiotic Stress Tolerance in Plants Through Microbial Association

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