Design and Characterization of a Silk-Fibroin-Based Drug Delivery Platform Using Naproxen as a Model Drug

Dyakonov, Tatyana; Yang, Chue Hue; Bush, Derek B.; Gosangari, Saujanya L.; Majuru, Shingai; Fatmi, Aqeel A.

doi:10.1155/2012/490514

Cited by 60 publications

(38 citation statements)

References 26 publications

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“…The silk fibroin was used as material for tissue engineering for skin, bone, blood vessel, ligament and nerve tissue regeneration, because it is non-immunogenic and non-toxic material. Some research articles show that silk fibroin is biodegradable, but the degradation rate of silk fibroin is not yet clear [163,[173][174][175][176][177][178][179][180][181][182]. The silk production is a natural ability of some arachnids, such as spiders, including some fossil species.…”

Section: Silk Proteins (Fibroin and Sericin) And Spider Silkmentioning

confidence: 99%

Biological synthesis of metallic nanoparticles: plants, animals and microbial aspects

Das

Pachapur

Lonappan

et al. 2017

Nanotechnol. Environ. Eng.

388

138

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The green synthesis (GS) of different metallic nanoparticles (MNPs) has re-evaluated plants, animals and microorganisms for their natural potential to reduce metallic ions into neutral atoms at no expense of toxic and hazardous chemicals. Contrary to chemically synthesized MNPs, GS offers advantages of enhanced biocompatibility and thus has better scope for biomedical applications. Plant, animals and microorganisms belonging to lower and higher taxonomic groups have been experimented for GS of MNPs, such as gold (Au), silver (Ag), copper oxide (CuO), zinc oxide (ZnO), iron (Fe 2 O 3 ), palladium (Pd), platinum (Pt), nickel oxide (NiO) and magnesium oxide (MgO). Among the different plant groups used for GS, angiosperms and algae have been explored the most with great success. GS with animal-derived biomaterials, such as chitin, silk (sericin, fibroin and spider silk) or cell extract of invertebrates have also been reported. Gram positive and gram negative bacteria, different fungal species and virus particles have also shown their abilities in the reduction of metal ions. However, not a thumb rule, most of the reducing agents sourced from living world also act as capping agents and render MNPs less toxic or more biocompatible. The most unexplored area so far in GS is the mechanism studies for different natural reducing agents expect for few of them, such as tea and neem plants. This review encompasses the recent advances in the GS of MNPs using plants, animals and microorganisms and analyzes the key points and further discusses the pros and cons of GS in respect of chemical synthesis.

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Section: Silk Proteins (Fibroin and Sericin) And Spider Silkmentioning

confidence: 99%

Biological synthesis of metallic nanoparticles: plants, animals and microbial aspects

Das

Pachapur

Lonappan

et al. 2017

Nanotechnol. Environ. Eng.

388

138

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“…Once purified, fibroin can be dissolved in a number of aqueous, chaotropic high concentration salts (e.g., ≥9 M LiBr, ≥50°C or ≥10 M LiSCN, ≥25°C) or ternary systems containing alcohols (e.g., CaCl 2 –water-ethanol, ≥70°C). Subsequent desalting of high-salt fibroin solutions is generally carried out by dialysis to obtain an aqueous regenerated fibroin solution, though chromatographic desalting protocols have also been proposed [70]. …”

Section: Desirable Properties Of Silk For Sustained Drug Deliverymentioning

confidence: 99%

Silk-based biomaterials for sustained drug delivery

Yücel

Lovett

Kaplan

2014

Journal of Controlled Release

302

216

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Silk presents a rare combination of desirable properties for sustained drug delivery, including aqueous-based purification and processing options without chemical cross-linkers, compatibility with common sterilization methods, controllable and surface-mediated biodegradation into non-inflammatory by-products, biocompatibility, utility in drug stabilization, and robust mechanical properties. A versatile silk-based toolkit is currently available for sustained drug delivery formulations of small molecule through macromolecular drugs, with a promise to mitigate several drawbacks associated with other degradable sustained delivery technologies in the market. Silk-based formulations utilize silk’s well-defined nano- through microscale structural hierarchy, stimuli-responsive self-assembly pathways and crystal polymorphism, as well as sequence and genetic modification options towards targeted pharmaceutical outcomes. Furthermore, by manipulating the interactions between silk and drug molecules, near-zero order sustained release may be achieved through diffusion- and degradation-based release mechanisms. Because of these desirable properties, there has been increasing industrial interest in silk-based drug delivery systems currently at various stages of the developmental pipeline from pre-clinical to FDA-approved products. Here, we discuss the unique aspects of silk technology as a sustained drug delivery platform and highlight the current state of the art in silk-based drug delivery. We also offer a potential early development pathway for silk-based sustained delivery products.

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“…27 Preparation of sF solution SF solution was prepared as previously described. 28,29 Briefly, Bombyx mori silkworm cocoons were cut into small pieces and boiled for 30 minutes in a solution of 0.5 wt% Na 2 CO 3 . After three rinses with ultrapure water, the extracted fibroin fibers were then dried at 60°C for 6 hours.…”

Section: Synergy Of Ic and Bmp2mentioning

confidence: 99%

Evaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system

Wang¹,

Cao²,

Liu³

et al. 2017

IJN

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BMP2 is well known as an outstanding growth factor for inducing new bone formation. However, improvements are still required to use BMP2 effectively and expand its clinical application due to the potential side effects at high doses. In this study, icariin (IC), a type of traditional Chinese medicine, was originally proposed to be a cooperative factor for BMP2. An alkaline phosphatase (ALP) activity assay showed that IC promoted BMP2 osteogenesis in a concentration-dependent manner with significant enhancement at 38.4 µM versus that for BMP2 at 0.8 µg/mL. Furthermore, we developed a composite hierarchical porous scaffold (SF/SBA15; composed of micropores of silk fibroin [SF] scaffold and mesopores of SBA15) for the controlled delivery of BMP2 and IC. This composite scaffold was investigated by a series of physical characterizations and displayed good in vitro cell biocompatibility. In addition, the composite scaffold also showed the degradation rate of 12% dry weight loss and a slight change in the microstructures within 10 days. Moreover, BMP2 and IC were loaded into the SF and SBA15 structures, respectively, of the SF/SBA15 scaffold. This protein/drug loading system (SF BMP2 /SBA15 IC ) provided delivery of BMP2 with an initial burst release of 60.9%±0.9% in the first 24 hours and a gradual release over the subsequent 6 days to 97.9%±0.8%, whereas IC exhibited a burst release of 64.2%±0.7% in the first 24 hours, followed by a sustained release to 92.4%±0.8% over 10 days. With the prolonged local retention and interaction duration of BMP2 and IC, the SF BMP2 /SBA15 IC scaffold provided better osteogenic differentiation than other groups with different loading modes of BMP2 or IC, as determined by ALP staining and quantitation and Alizarin red staining. Finally, the results of quantitative real-time polymerase chain reaction analysis indicated that the SF BMP2 /SBA15 IC scaffold induced a significantly higher increase in the RUNX2, ALP, COL I , and OCN expression levels of cocultured bone marrow mesenchymal stem cells than other payload composite scaffolds. This study suggests that a micro/meso hierarchical porous delivery system of BMP2 and IC ensures osteogenic synergy and demonstrates promise for bone tissue engineering.

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Design and Characterization of a Silk-Fibroin-Based Drug Delivery Platform Using Naproxen as a Model Drug

Cited by 60 publications

References 26 publications

Biological synthesis of metallic nanoparticles: plants, animals and microbial aspects

Biological synthesis of metallic nanoparticles: plants, animals and microbial aspects

Silk-based biomaterials for sustained drug delivery

Evaluation of synergistic osteogenesis between icariin and BMP2 through a micro/meso hierarchical porous delivery system

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