Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex

Albastaki, Abdulla; Naji, Mohammed; Lootah, Reem; Almheiri, Reem; Almulla, Hanan; Alreyami, Afra; Almarri, Iman; Yu, Zailiang; Zhang, Yueke; Chen, Shu; Alghafri, Rashed

doi:10.1002/elps.202200099

Cited by 7 publications

(3 citation statements)

References 19 publications

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“…A number of Y-STR typing systems have been described along with validation studies, such as a 36plex [ 453 ], a 41plex [ 454 ], a 29plex [ 455 ], a 17plex [ 456 ], a 24plex [ 457 ], the Microreader 40Y ID System [ 458 ], the 24 Y-STRs in the AGCU Y SUPP STR kit [ 459 ], the DNATyper Y26 PCR amplification kit [ 460 ], a multiplex with 12 multicopy Y-STR loci [ 461 ], the Yfiler Platinum PCR Amplification Kit [ 462 ], a 45plex [ 463 ], the Microreader 29Y Prime ID system [ 464 ], an assay with 30 slow and moderate mutation Y-STR markers [ 465 ], the 17plex Microreader RM-Y ID System [ 466 ], and a 26plex for rapidly mutating Y-STRs [ 467 ]. A machine learning program predicted Y haplogroups using two Y-STR multiplexes with 32 Y-STRs [ 468 ].…”

Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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“…The focal point of this research is a set of 13 autosomal STRs markers, including clusters of closely linked markers, designed to overcome the limitations of routine autosomal chromosome STRs loci. A multiplex PCR system was developed to simultaneously analyze these markers, aiming to enhance their applicability in forensic contexts (Albastaki et al, 2022). The primary objective is to evaluate the effectiveness of this set of linked STRs for kinship analysis within the Libyan population (Wyner et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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2024

NSJ

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This novel study investigates the utilization of a centromeric short tandem repeat (STR) marker to examine the level of homozygosity within the Libyan population. Building upon previous research that highlights challenges in kinship analysis using routine autosomal chromosomal markers. The STRs loci investigation evaluates the efficacy of 15 autosomal STRs markers and how these markers can be used to explore the implications of homozygosity in the context of consanguinity and its potential association with increasing instances of cancer risks. This study focuses on assessing the level of inbreeding within the Libyan population through the utilization of centromeric short tandem repeats (STRs) markers. The aim is to investigate the frequency of homozygosity, particularly in the D19S433 marker located in the centromere region, among consanguineous groups compared to non-consanguineous groups. The centromere's role in recombination suppression and the potential implications for passing ancestral genes to offspring are explored. The study includes a comprehensive examination of DNA profiling, consanguinity, and its possible associations with cancer risks in the context of the unique genetic and demographic characteristics of the Libyan population.

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“…Thus, the rapidly mutating Y-chromosomal STRs (RM Y-STRs) have relatively high mutation rates (>1 × 10 2 mutation rates than common Y-STRs) and may play an important role in identifying related males. Until now, a large number of studies have been conducted on RM Y-STRs [13,14], which can provide a powerful tool for Y chromosome analysis in forensic genetics, including migratory events in the Luzhou area that would have affected the Y-STR haplotype diversity. In a previous study, our group investigated the 23 Y-STR mutation rates of this system in the southwest Han population [15].…”

Section: Introductionmentioning

confidence: 99%

Genetic Polymorphism Analysis of 24 Y-STRs in a Han Chinese Population in Luzhou, Southwest China

Fu,

Song,

Qian

et al. 2023

Genes

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Han is the largest of China’s 56 ethnic groups and the most populous ethnic group in the world. The Luzhou region is located in southwest China, at the junction of three provinces. The unique historical factors contribute to the genetic polymorphism information. Short tandem repeats (STRs) are highly polymorphic, but the polymorphism of the Y chromosomal STRs (Y-STRs) loci in the Luzhou region is still unclear. It is of great significance to provide Y-STRs genetic data for the Han population from the Luzhou areas of southwest China. A total of 910 unrelated male individuals of the Han population from the Luzhou area were recruited, and 24 Y-STRs were analyzed. The population structure and phylogenetic relationships were compared with those of another 11 related Han populations. A total of 893 different haplotypes were achieved from 910 samples, of which 877 (98.21%) haplotypes were unique. Haplotype diversity and discrimination were 0.999956 and 0.981319, respectively. The lowest genetic diversity of DYS437 is 0.4321, and the highest genetic diversity of DYS385a/b is 0.9642. Pair-to-pair genetic distance and relative probability values indicate that Luzhou Han people are close to Sichuan Han people, Guangdong Han people, and Hunan Han people, which is consistent with geographical distribution, historical influence, and economic development. The 24 Y-STR markers of the southwest Luzhou Han population were highly polymorphic, which provided us with genetic polymorphism information and enriched the population genetic database. Therefore, it is of great value to our forensic applications and population genetics research.

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Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex

Cited by 7 publications

References 19 publications

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

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Genetic Polymorphism Analysis of 24 Y-STRs in a Han Chinese Population in Luzhou, Southwest China

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