Design and evaluation of cabazitaxel loaded NLCs against breast cancer cell lines

Chand, Pallavi; Kumar, Hitesh; Badduri, Nihal; Gupta, N. Vishal; Bettada, Vidya G.; Madhunapantula, SubbaRao V.; Kesharwani, Siddharth S.; Dey, Sangeeta; Jain, Vikas

doi:10.1016/j.colsurfb.2020.111535

Cited by 39 publications

(30 citation statements)

References 42 publications

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“…The mean particle size, PDI, and ζ of the formulated PNPs were evaluated through the DLS method using Malvern zetasizer [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, 0.5 mL PAX-RESV loaded PNPs formulation were centrifuged at 14,000 rpm for 30 min at 4 °C in an ultracentrifuge. Then, 50 µL of supernatant was taken out by micropipette and diluted with 5 mL of HPLC grade ACN for estimating the amount of free PAX and RESV by the RP-HPLC method [ 17 ]. The %EE was calculated by using the following Equation (1): %EE = Amount of initial drug concentration − unentrapped drug in supernatant ÷ Amount of initial concentration × 100 …”

Section: Methodsmentioning

confidence: 99%

“…Aliquot (100 µL) of samples were withdrawn at various timepoints (0.1, 0.2, 0.3, 0.4, 0.5, 1, 2, 3, 4, 8, 12, 24, 36 h), and a net equal amount PBS was replaced to maintain constant release volume. The unknown concentrations of PAX and RESV were estimated by reverse phase HPLC technique (LC-2030C, Shimadzu, Japan) [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Polymeric loaded nanoparticles containing single drug (PAX and RESV) were also prepared using the above procedure with drug: Soluplus: TPGS1000 ratio being 1:30:1 mg•mL −1 . The mean particle size, PDI, and ζ of the formulated PNPs were evaluated through the DLS method using Malvern zetasizer [17].…”

Section: Preparation Of Functionalized Polymeric Nanoparticlesmentioning

confidence: 99%

“…This bag was placed beaker containing 100 mL of 0.2% Tween 80 PBS with continuous stirring at 100 rpm that was maintained at a temperature of 37 ± 0.5 • C. Aliquot (100 µL) of samples were withdrawn at various timepoints (0.1, 0.2, 0.3, 0.4, 0.5, 1, 2, 3, 4, 8, 12, 24, 36 h), and a net equal amount PBS was replaced to maintain constant release volume. The unknown concentrations of PAX and RESV were estimated by reverse phase HPLC technique (LC-2030C, Shimadzu, Japan) [17].…”

Section: In Vitro Release Studiesmentioning

confidence: 99%

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Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management

et al. 2021

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Gliomas are one of the prominent cancers of the central nervous system with limited therapeutic modalities. The present investigation evaluated the synergistic effect of paclitaxel (PAX) and resveratrol (RESV)-loaded Soluplus polymeric nanoparticles (PNPs) against glioma cell lines along with in vivo pharmacokinetics and brain distribution study. PAX-RESV-loaded PNPs were prepared by the thin film hydration technique and optimized for different dependent and independent variables by using DoE (Design-Expert) software. The in vitro physiochemical characterization of prepared PAX-RESV-loaded PNPs exhibited appropriate particle size, PDI and % encapsulation efficiency. Cytotoxicity assay revealed that PTX-RESV loaded PNPs had a synergistic antitumor efficacy against C6 glioma cells compared with single and combined pure drugs. Finally, the pharmacokinetic and brain distribution studies in mice demonstrated that the PNPs significantly enhanced the bioavailability of PTX-RESV PNPs than pure PAX and RESV. Thus, the study concluded that PAX-RESV PNPs combination could significantly enhance anti-glioma activity, and this could be developed into a potential glioma treatment strategy.

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“…The mean particle size, PDI, and ζ of the formulated PNPs were evaluated through the DLS method using Malvern zetasizer [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Functionalized Polymeric Nanoparticlesmentioning

confidence: 99%

Section: In Vitro Release Studiesmentioning

confidence: 99%

See 3 more Smart Citations

Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management

et al. 2021

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Enhancing high‐temperature stability of limonene‐loaded nanostructured lipid carriers with various solid lipids

Feng,

Tian,

Sheng

et al. 2024

Food Bioengineering

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In this research, nanostructured lipid carriers (NLCs) loaded with limonene were developed using various solid lipids. The impact of high temperatures on the characteristics of NLCs was investigated. NLCs exhibited zeta potential values exceeding |30| mV, indicating excellent homogeneity and stability. Increasing the carbon chain length of monoglycerides from C15 to C21 resulted in a corresponding increase in particle size of NLCs from 219.1 ± 1.1 to 243.3 ± 0.9 nm. However, the particle size remained relatively constant with an increase in the number of solid lipid carbon chains. Encapsulation efficiency of limonene increased from 66.0 ± 0.7% to 86.2 ± 0.8% with an increase in the number of solid lipid carbon chains. The result showed that more ester bonds facilitated the dissolution of the target and enhanced the interaction forces between solid lipids and the target. X‐ray diffraction, Fourier transform‐infra‐red spectroscopy and differential scanning calorimetry analyses confirmed effective encapsulation of limonene in NLCs, resulting in good stability. NLCs prepared from various solid lipids exhibited varying properties. Glycerol triglyceride demonstrated superior stability and homogeneity of nanoparticles under high‐temperature conditions compared to other solid lipids. This study provides enhancing the thermal stability of limonene‐loaded NLCs and proposes a novel approach for their practical application.

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Preparation, structural and morphological characterization of cartilage type II collagen peptide assemblies from sturgeon head

Cui,

Shao,

et al. 2024

J Sci Food Agric

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BACKGROUNDSturgeon cartilage type II collagen peptides (SHCPs) can self‐assemble and be used to prepare collagen peptide assemblies. Self‐assembled peptides have great potential for applications in the food industry. In the present study, self‐assembled peptides were prepared from sturgeon cartilage and then characterized.RESULTSThe SHCPs self‐assembled and formed collagen peptide assemblies. After response surface experiment optimization, the optimal enzyme digestion process comprised 43.1 °C, 3.37 h and 0.96% enzyme addition, and the peptide yield was 78.46%. Physicochemical analysis showed that the SHCPs were amphiphilic, with an average molecular weight of 1081 Da, and were rich in hydrophobic amino acids. Peptide sequence identification showed that the peptides of SHCPs with polar amino acids followed by hydrophobic amino acids could be self‐assembled through hydrogen bonding and hydrophobic interaction. Through turbidity experiments, Fourier transform infrared spectroscopy and scanning electron microscopy, we demonstrated that SHCPs can self‐assemble into reticular and tubular structures under specific conditions. Furthermore, both the SHCPs‐Ca and SHCPs‐Mg assemblies were stabilized within a pH range consistent with that of the human gastrointestinal tract.CONCLUSIONThe present study provides a simple and safe method for preparing novel self‐assembled peptide materials from sturgeon by‐products, providing a scientific basis for the exploitation of sturgeon cartilage and potentially reducing resource wastage. © 2024 Society of Chemical Industry.

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Design and evaluation of cabazitaxel loaded NLCs against breast cancer cell lines

Cited by 39 publications

References 42 publications

Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management

Fabrication and Characterization of Paclitaxel and Resveratrol Loaded Soluplus Polymeric Nanoparticles for Improved BBB Penetration for Glioma Management

Enhancing high‐temperature stability of limonene‐loaded nanostructured lipid carriers with various solid lipids

Preparation, structural and morphological characterization of cartilage type II collagen peptide assemblies from sturgeon head

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