Design and Experimental Approach to the Construction of a Human Signal-Molecule-Profiling Database

Zhao

2015

Anal. Chem.

Self Cite

The incorporation of pathogen identification with antimicrobial susceptibility testing (AST) was implemented on a concept microfluidic simulator, which is well suited for personalizing antibiotic treatment of urinary tract infections (UTIs). The microfluidic device employs a fiberglass membrane sandwiched between two polypropylene components, with capture antibodies immobilized on the membrane. The chambers in the microfluidic device share the same geometric distribution as the wells in a standard 384-well microplate, resulting in compatibility with common microplate readers. Thirteen types of common uropathogenic microbes were selected as the analytes in this study. The microbes can be specifically captured by various capture antibodies and then quantified via an ATP bioluminescence assay (ATP-BLA) either directly or after a variety of follow-up tests, including urine culture, antibiotic treatment, and personalized antibiotic therapy simulation. Owing to the design of the microfluidic device, as well as the antibody specificity and the ATP-BLA sensitivity, the simulator was proven to be able to identify UTI pathogen species in artificial urine samples within 20 min and to reliably and simultaneously verify the antiseptic effects of eight antibiotic drugs within 3-6 h. The measurement range of the device spreads from 1 × 10(3) to 1 × 10(5) cells/mL in urine samples. We envision that the medical simulator might be broadly employed in UTI treatment and could serve as a model for the diagnosis and treatment of other diseases.

Section: ■ Future Outlookmentioning

confidence: 99%

Section: Future Outlookmentioning

confidence: 99%

Rapid Identification and Susceptibility Testing of Uropathogenic Microbes via Immunosorbent ATP-Bioluminescence Assay on a Microfluidic Simulator for Antibiotic Therapy

Zhao

2015

Anal. Chem.

Self Cite

“…A portable integrated electrochemical sensor for rapid diagnosis of UTI will be designed and developed for proving accurate nitrite quantification directly on diaper without extracting liquid out [13]. Series of artificial urine samples with different amount of spiked E.coli will be studied respectively, thereby proving a distinct mechanism how the amount of E.coli was related to the nitrite concentration in human urine.…”

Section: Future Workmentioning

confidence: 99%

Applied Technology in Diaper-Based UTI Testing for Elder People by Using Nitrite Ion Selective Electrode

Feng

2014

AMM

Self Cite

Urinary tract infections (UTI) are common infection diseases in the hospitals, especially for the elder people. Detecting UTI for the elder people is difficult and time-consuming procedure. Since measuring nitrite directly in urine is often employed in the diagnosis of UTI, a nitrite ion selective electrode (ISE) was investigated in this study in order to detect UTI samples directly on used diapers of patients. With a commercial ISE sensor for nitrite, the detecting system was built to quickly and precisely quantify different concentrations of nitrite ions in both water solutions and real human urine samples. Good results were shown in the experiment that the nitrite can be precisely detected in the fresh urine by the ISE with the relations as Ε=224.7-21.259×Lg (C+13.476), where E is the electrode potential and C is the concentration of nitrite ions. Besides, the method of extracting urine samples from used diapers was also studied for further research. The investigation suggested that a sampling module for used diapers could be simply realized if necessary.

“…After that, the signals were visualized by Labview on the PC. the data of UTI can easily observed and shared [16].…”

Section: Building Of the Detecting Systemmentioning

confidence: 99%

Detection of Urinary Tract Infections on lab-on-chip device by measuring photons emitted from ATP bioluminescence

Feng

2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Yang

2014

A microfluidic Lab-on-chip (LOC) platform for in vitro detecting Urinary Tract Infections (UTI) for clinical diagnostic applications has been built. Based on one commercial adenosine 5'-triphosphate (ATP) assay kit, one chip designed before was applied to detect UTI with the help of photomultiplier tube (PMT) and quantitative determination was made by measuring the photons of light emitted in the bioluminescent reaction of ATP with the enzyme luciferase. The chip had been tested and materials had been well prepared before testing the PMT detecting system. The data from PMT were visualized by the Labview™, appearing good linearity between voltage values and the concentration of the ATP ranging from 2×10(-12) M to 2×10(-8) M. Fresh urine sample with different amounts of Escherichia coli had been measured by the system, appearing good linearity trend between the voltage values and number of the E.coli. This study successfully expressed the concept of measuring ATP directly in the urine to quickly and accurately detect UTI on a microfluidic chip.