Design and Implementation of Anatomically Inspired Mesenteric and Intestinal Vascular Patterns for Personalized 3D Bioprinting

Abstract

Recent progress in bioprinting has made possible the creation of complex 3D intestinal constructs, including vascularized villi. However, for their integration into functional units useful for experimentation or implantation, the next challenge is to endow them with a larger-scale, anatomically realistic vasculature. In general, the perfusion of bioprinted constructs has remained difficult, and the current solution is to provide them with mostly linear and simply branched channels. To address this limitation, here we demonstrated an image analysis-based workflow leading through computer-assisted design from anatomic images of rodent mesentery and colon to the actual printing of such patterns with paste and hydrogel bioinks. Moreover, we reverse-engineered the 2D intestinal image-derived designs into cylindrical objects, and 3D-printed them in a support hydrogel. These results open the path towards generation of more realistically vascularized tissue constructs for a variety of personalized medicine applications.

Engineering programmable material-to-cell pathways via synthetic notch receptors to spatially control differentiation in multicellular constructs

“…6F, G ). We also designed a pattern to replicate a branching network structure typical of vascular beds 48 and showed the formation of a tissue consisting of activated cells in the corresponding pattern surrounded by a uniform layer of fibroblasts (Figs. 6H and S7F ).…”

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

“…Different from support baths composed of particles, continuous-phase support baths are composed of polymer Xanthan Gum [43] Decellularized extracellular matrix (dECM) [144,145] Embryoid body matrix [23] Gelatin methacrylate (GelMA)/ poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) [146] Collagen [18,24,47,56,[140][141][142]144,[147][148][149][150][151][152][153] Fibrin, Fibrinogen [24,56,148,154] Hyaluronic acid (HA), functionalized/modified HA [18,149,[154][155][156][157][158] Cells, spheroids [18,155] Locust bean gum / Paracetamol Locust bean gum / Ibuprofen [159] i-carrageenan [47] Gellan gum [47] Cellulose nanofibrils / Carbon nanotubes / Alginate [160] Polyethylene glycol (PEG), functionalized PEG [114,148,155,161,162] Laminin [150] Gallium−Indium / Alginate, Polyester, Silk, Stainless steel …”

Engineering Programmable Material-To-Cell Pathways Via Synthetic Notch Receptors To Spatially Control Cellular Phenotypes In Multi-Cellular Constructs

“…6D-E, when anti-GFP synNotch cells that induce ETV2-BFP were cultured on these surfaces, the transgene was induced only locally on the patterns at day 1 and day 3. We then designed a pattern to replicate a branching network structure typical of vascular beds 48 and showed the formation of a tissue consisting of activated cells in the corresponding pattern surrounded by a uniform layer of fibroblasts (Fig. 6F and S7D).…”