Design and Implementation of Experimental Consumable Management Device for the Automatic Nucleic Acid Detection System

Jun, Yu; Zhu, Caichao; Wang, Chao; Yana, HU; Dong, Hongming; Deng, Yan

doi:10.1166/jnn.2016.13033

Cited by 6 publications

(1 citation statement)

References 27 publications

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“…[1] introduces a monitoring system via RS485 serial communication protocol. [2] designs an Automatic Nucleic Acid Detection System though RS485 bus. The low-voltage meter reading system is based on RS485 interface [3].…”

Section: Introductionmentioning

confidence: 99%

An on-chip TVS design to meet system level ESD protection for RS485 transceiver

Zhou

Zhang²,

Yuan³

et al. 2021

JER is an international, peer-reviewed journal that publishes f

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RS485 interface is widely used in the area of industrial control and remote meter reading, which are often subjected to serious electrostatic damage. A new On-Chip TVS (OCT) structure without extra process modification and a novel electrostatic discharge method for RS485 transceiver IC have been proposed. It is composed of a serie of Zener diodes and fabricated in 5V/18V/24V 0.5µm CDMOS technology. A 100ns pulse width of the Transmission Line Pulsing (TLP) test is performed for this proposed OCT. The driver circuit itself can work as an ESD device as well. The OCT trigger voltage is compatible with the signal level of RS485 standard. The OCT device protection level of human-body-model (HBM) is up to 16.34kV. RS485 transceiver integrated with OCT has also been tested in order to verify its reliability. The results indicate that it can pass the IEC61000-4-2 contact ±10kV stress and IEC 61000−4−4 Electrical Fast Transient (EFT) ±2.2kV without any hard damages and latch-up issues. The RS485 transceiver integrated with the OCT allows error-free data rate transfer up to 500 kbps. The chip occupies a silicon area of 2.4×1.17mm2.

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Section: Introductionmentioning

confidence: 99%

An on-chip TVS design to meet system level ESD protection for RS485 transceiver

Zhou

Zhang²,

Yuan³

et al. 2021

JER is an international, peer-reviewed journal that publishes f

View full text Add to dashboard Cite

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Design and Application of a Portable Multi-Channel Turbidimeter by Loop-Mediated Isothermal Amplification

Yang¹,

Wu²,

Li³

et al. 2017

nanosci nanotechnol lett

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With the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. A device has been developed for real-time monitoring the process of loop-mediated isothermal amplification reaction through photoelectric detection technology and temperature control technology. This paper aimed at the designing protocol and the application of real-time turbidity detection system by LAMP by means of that magnesium ions can combine pyrophosphate ions by-product of LAMP reaction to form a white precipitate of magnesium pyrophosphate. The change of sample turbidity can be detected by a photoelectric sensor. The system is easy and friendly to operate and the amplification process of 8 samples can be completed simultaneously at 63 °C in one hour. In the end, the experiment results suggest that the real-time turbidity detection system is stable and reliable.

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Up-Regulation of miR-96-5p Inhibits the Proliferation of FaDu Cell Line by Targeting mTOR

Liao¹,

Ling²,

Jiang³

et al. 2017

nanosci nanotechnol lett

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Hypopharyngeal carcinoma is an upper respiratory-gastrointestinal tract cancer, with a complicated pathogenesis and high mortality. We predicted that hsa-miR-96-5p targeted mTOR. To test this hypothesis, the overexpression vector of miR-96-5p, mTOR 3'UTR and its mutant vector with cobinding sites of miR-96-5p, and shRNA-mTOR vectors were constructed. Two groups of FaDu cells were transfected with and without metformin (10 mmol/L), and cultivated for 48, 72 and 96 hours. RTPCR and Western blotting experiments showed that upregulation of miR-96-5p inhibited proliferation of FaDu cells. At the same time, we found that upregulation of miR-96-5p inhibited the growth of FaDu cells treated with metformin. After detecting the mTOR mutant 3'UTR loci by dual luciferase assay, we found that the recorded fluorescence values were much higher than that of the wildtype. We also determined that the ratio of firefly fluorescence value/Renilla fluorescence value was significantly higher than that of wild-type, indicating that mTOR was the direct target of the miR-965-p gene. In conclusion, miR-96-5p inhibited proliferation of FaDu cells by targeting mTOR, and it may serve as a potential target for tumor growth inhibition.

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Design and Implementation of Experimental Consumable Management Device for the Automatic Nucleic Acid Detection System

Cited by 6 publications

References 27 publications

An on-chip TVS design to meet system level ESD protection for RS485 transceiver

An on-chip TVS design to meet system level ESD protection for RS485 transceiver

Design and Application of a Portable Multi-Channel Turbidimeter by Loop-Mediated Isothermal Amplification

Up-Regulation of miR-96-5p Inhibits the Proliferation of FaDu Cell Line by Targeting mTOR

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