Design and optimization of a lactate amperometric biosensor based on lactate oxidase cross-linked with polymeric matrixes

Romero, Marcelo Ricardo; Garay, Fernando; Baruzzi, Ana María

doi:10.1016/j.snb.2007.12.044

Cited by 65 publications

(69 citation statements)

References 39 publications

(54 reference statements)

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“…Each biosensor was washed with Milli-Q water and then rinsed before experiments. Control experiments (MWCNT-SPEs and H 2 O 2 [20], MWCNT-SPEs and l-lactate [21], enzyme adsorbed on SPEs and l-lactate [21]) confirm that l-lactate electrochemical detection drastically improves by the use of MWCNTs with adsorbed l-lactate oxidase.…”

Section: Electrode Preparationmentioning

confidence: 69%

“…Some previous examples for the amperometric biosensing of l-lactate have been described using l-lactate oxidases (FMNdependent) and l-lactate dehydrogenases (NAD + -dependent) from various natural sources. However, for the described biosensors polymeric matrices [16], sol-gel immobilization techniques [17], hydrogels or glutaraldehyde were used for enzyme immobilization [15].…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Taurino

Reiss

Richter

et al. 2013

Electrochimica Acta

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Keywords:l-Lactate oxidases from Aerococcus viridans Engineered and commercial enzymes Multi-walled carbon nanotubes Enzyme biosensor a b s t r a c t A comparison between engineered and commercially available l-lactate oxidases from Aerococcus viridans was conducted for biosensing applications. Enzymes were adsorbed onto the surfaces of graphite electrodes modified with multi-walled carbon nanotubes. Thermostable l-lactate oxidases were cloned with a (i) N-, (ii) a C-terminal His-tag and (iii) a wild-type enzyme. Subsequently to the heterologous expression in Escherichia coli and purification, we determined the kinetic parameters of these enzymes in solution. The kinetics of the wild-type, of the N-terminally His-tagged enzyme and of the commercial l-lactate oxidase from A. viridans were studied with a classical Michaelis-Menten as well as with a substrate inhibition model, while the enzyme carrying a C-terminal His-tag showed no activity. The active enzymes were used to fabricate and comparatively investigate multi-walled carbon nanotubes-based biosensors. The enzyme kinetic results were compared with electrochemical studies. By using both spectrophotometric and amperometric techniques, the inhibition phenomenon fits better to the data especially those data related with Lox-His-N. The electrochemical data of the fabricated enzymatic biosensors showed that the N-terminally His-tagged l-lactate oxidase performed best on carboxyl-modified carbon nanotubes. The sensor based on this engineered enzyme showed the highest sensitivity and lowest detection limit in the range of l-lactate concentration 0-1 mM as well as long term stability over one month.

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Section: Electrode Preparationmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Taurino

Reiss

Richter

et al. 2013

Electrochimica Acta

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“…Different solutions have been proposed in literature to design and optimize amperometric biosensors dedicated to the lactate detection [2], [3]. The use of carbon nanotubes has been proposed to enhance the sensitivity of these devices [4], [5].…”

Section: A Remote Powering Of Lactate Biosensorsmentioning

confidence: 99%

“…We report here the analytical formulas presented in [17] to describe the link efficiency as a function of the electrical parameters of the link. By defining as the power dissipated on the secondary circuit over the totally dissipated power (1) and as the power dissipated on the load over the power dissipated on the secondary circuit (2) the link efficiency can be written as…”

Section: A Link Efficiencymentioning

confidence: 99%

A Study of Multi-Layer Spiral Inductors for Remote Powering of Implantable Sensors

Olivo

Carrara

Micheli

2013

IEEE Trans. Biomed. Circuits Syst.

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Abstract-An approach based on multi-layer spiral inductors to remotely power implantable sensors is investigated. As compared to single-layer inductors having the same area, multi-layer printed inductors enable a higher efficiency (up to 35% higher) and voltage gain (almost one order of magnitude higher). A system conceived to be embedded into a skin patch is designed to verify the performance. The system is able to transmit up to 15 mW over a distance of 6 mm and up to 1.17 mW where a 17 mm beef sirloin is placed between the inductors. Furthermore, the system performs downlink communication (up to 100 kbps) and uplink communication based on the backscattering technique (up to 66.6 kbps). Long-range communication is achieved by means of a bluetooth module.Index Terms-Energy harvesting, implantable sensors, inductive link, multi-layer spiral inductors, remote powering.

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“…On the other hand there has been increasing research on lactate biosensors mainly because its determination in blood allows predicting multiple organ failure and death of patient with septic shock. Lactic acidosis is known to accompany decreased tissue oxygenation and drug toxicity, and the analysis of lactate can be used as a preliminary diagnostic for cystic fibrosis, metabolic disorders, thrombosis and physical condition of athletes [15,16]. In addition to the relevance in clinical diagnosis, the determination of glucose and lactate is very important in other areas such as fermentation control and food analysis [17,18].…”

Section: Introductionmentioning

confidence: 99%

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

et al. 2011

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The bioelectrocatalytical properties and kinetic characteristics of new oxidase biosensors based on two different carbosilane dendrimers are described. The best glucose biosensor developed displayed, in an ascorbate interference free work potential interval, a strictly linear range from 0 to 4.0 mM, a detection limit of 40,6 mM and a response time less than 3 s. The lactate biosensor displayed a linear range from 0 to 0.8 mM, a detection limit of 0.73 mM and a response time less than 2 s. The apparent Michaelis-Menten constants were calculated to be 4.39 mM and 2.08 mM respectively, according to Lineweaver-Burk equation.

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Design and optimization of a lactate amperometric biosensor based on lactate oxidase cross-linked with polymeric matrixes

Cited by 65 publications

References 39 publications

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

Comparative study of three lactate oxidases from Aerococcus viridans for biosensing applications

A Study of Multi-Layer Spiral Inductors for Remote Powering of Implantable Sensors

Multioperational Oxidase Biosensors Based on Carbosilane Dendrimers with Interacting Ferrocenes

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