Design‐based stereological estimation of hepatocyte number, by combining the smooth optical fractionator and immunocytochemistry with anti‐carcinoembryonic antigen polyclonal antibodies

Marcos, Ricardo; Monteiro, R. A. F.; Rocha, Eduardo

doi:10.1111/j.1478-3231.2005.01201.x

Cited by 42 publications

(50 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, and facing some established criteria (e.g., Ikeda et al 1995;Majno and Joris 1995), we observed typical morphological aspects of apoptosis attaining hepatocytes. However, the quantification of such phenomena was not a target when we began the study, but it can be addressed in the future, e.g., by combining immunochemistry with an optical fractionator design in paraffin sections (Marcos et al 2004(Marcos et al , 2006. Anyway, our data support that a numerical decline of hepatocytes in females from vitellogenesis to post-spawning occurred.…”

Section: Discussionsupporting

confidence: 61%

“…Such concepts have been largely implemented in neuroscience-to the point that editors urged authors and reviewers to adhere to the so-called ''stereologically based unbiased estimates'' (Coggeshall and Lekan 1996;Saper 1996). Their use in hepatology is apparently confined to only a few studies, made in rats (Jack et al 1990a, b;Marcos et al 2003Marcos et al , 2004Marcos et al , 2006 and in brown trout (Rocha et al , 2001; therefore, more applications are needed to gain new insights using more powerful quantitative tools.…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

The hepatocytes of the brown trout (Salmo trutta f. fario): a stereological study of their number and size during the breeding cycle

Rocha

Galante

et al. 2008

Ichthyol Res

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 96%

The hepatocytes of the brown trout (Salmo trutta f. fario): a stereological study of their number and size during the breeding cycle

Rocha

Galante

et al. 2008

Ichthyol Res

Self Cite

View full text Add to dashboard Cite

“…Six random dissector pairs were analyzed for each specimen. The collagen fibers, fat globule and hepatocytes absolute and relative volume (V and Vv to [cf], [fg], and [h], respectively) as well as the absolute number of hepatocytes (N[h]) were determined as the product of the volume densities of these structures and the numerical density of the hepatocytes by the hepatic volume previously measured (Aguila et al, 2003;Marcos et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

Effects of Oral Nicotine on Rat Liver Stereology

2008

View full text Add to dashboard Cite

SUMMARY:Nicotine is the more abundant component in cigarette smoke. Because nicotine is first metabolized in the liver, our aim was to investigate the effects of nicotine on this organ by biochemical and stereological methods. Male Wistar rats were treated with oral nicotine (ON) diluted in drinking water during 32 days. The control group was treated with drinking water in the same period. Rats were sacrificed 24 hours after last day, the blood was collected and the liver was removed. Lipidogram was performed by enzymatic method and collagen fibers, fat globules and hepatocytes were count in the liver by stereological methods. We observed in control group preserved hepatocytes, with no presence of inflammatory cells. However in the ON group was possible to observe varied size of hepatocytes with cloudy cellular limits and histoarchitecture loss. Capillaries were fully of red blood cells. We observed also an increase of fat globules with small size. We observed in leucogram a reduction of leukocytes number (lymphocytes, neutrophils and monocytes) in the ON group in comparison to control group. The lipidogram showed an increase of triglycerides and total cholesterol for ON group when compared to control group. Moreover, a reduction of HDL-cholesterol was observed in ON group when compared to control group. Numerical density of hepatocytes was lesser in ON group when compared to control group. We suggest harmful effects of oral nicotine in liver induced by toxic mechanism with reduction of hepatocytes number and disturbance in lipid metabolism.

show abstract

“…[27] Since the recent development of stereological quantification methods, a large amount of numerical data has been readily obtained. [28,29] In the histological step of our study, dilatation of glomerular capillaries and large blood vessels was seen. In addition to tubular deformations, hyaline accumulation, necrosis, and vacuolar degenerations supported our stereological data.…”

Section: Discussionmentioning

confidence: 99%

Does Haloperidol Have Side Effects on Histological and Stereological Structure of the Rat Kidneys?

et al. 2009

View full text Add to dashboard Cite

Haloperidol, a typical antipsychotic, is the most commonly prescribed medication for the treatment of mental health problems such as agitation and psychosis. We attempted to determine the effects of haloperidol treatment on the kidneys of female rats. In addition, we aimed to estimate the numerical density, total number, and height of renal glomeruli and the volume and volumetric fractions of the cortex, medulla, and whole kidneys, and tried to determine whether there was a change in these stereological parameters depending on haloperidol treatment. Both the qualitative and quantitative histological features of the kidney samples were analyzed with conventional histopathological and modern stereological methods at the light microscopic level. The total number of glomeruli and numerical density of glomerulus in the haloperidoltreated groups was not changed by increasing the dose in comparison to the control group. The mean height of the glomerulus significantly increased, especially in low-dose groups. In the haloperidol-treated groups, the volumetric fractions of the cortex to the whole kidney of the rats were significantly decreased by increasing the dose. The volumetric fractions of the medulla to the whole kidney of the rats were increased significantly in parallel by the given dose. In addition, we present quantitative findings showing that haloperidol is associated with many alterations in rat kidneys. It was shown that haloperidol may lead to undesirable changes in the kidney after chronic treatment with especially high doses.

show abstract

Design‐based stereological estimation of hepatocyte number, by combining the smooth optical fractionator and immunocytochemistry with anti‐carcinoembryonic antigen polyclonal antibodies

Abstract: The strategy here presented provides a reliable method for accessing the N of HEP (distinguishing MnHEP from BnHEP) in situations in which these parameters are relevant, namely for evaluating the magnitude of an hyperplastic liver response from its very early onset.

Cited by 42 publications

References 38 publications

The hepatocytes of the brown trout (Salmo trutta f. fario): a stereological study of their number and size during the breeding cycle

The hepatocytes of the brown trout (Salmo trutta f. fario): a stereological study of their number and size during the breeding cycle

Effects of Oral Nicotine on Rat Liver Stereology

Does Haloperidol Have Side Effects on Histological and Stereological Structure of the Rat Kidneys?

Contact Info

Product

Resources

About