Design, Characterization, and Anticancer and Antimicrobial Activities of Mucoadhesive Oral Patches Loaded with Usnea barbata (L.) F. H. Wigg Ethanol Extract F-UBE-HPMC

Popovici, Violeta; Matei, Elena; Cozaru, Georgeta Camelia; Bucur, Laura; Gîrd, Cerasela Elena; Schröder, Verginica; Ozon, Emma Adriana; Mitu, Mirela Adriana; Musuc, Adina Magdalena; Petrescu, Simona; Atkinson, Irina; Rusu, Adriana; Mitran, Raul−Augustin; Anastasescu, Mihai; Caraiane, Aureliana; Lupuliasa, Dumitru; Aşchie, Mariana; Dumitru, Eugen; Badea, Victoria

doi:10.3390/antiox11091801

Cited by 7 publications

(9 citation statements)

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“…CLS Cell Lines Service GmbH (Eppelheim, Germany) was the purveyor of the CLS-354 (Human mouth squamous cell carcinoma from a 51-year-old male) growing culture and culture medium—Dulbecco’s Modified Eagle’s Medium (DMEM) High Glucose, supplemented with 10% Fetal Bovine Serum (FBS) 4.5 g/L glucose and L-glutamine. Thermo Fisher Scientific Inc. (Waltham, MA, USA) for the media for blood cells (Dulbecco’s phosphate buffered saline with MgCl 2 and CaCl 2 ), FBS, Trypsin-ethylenediamine tetra acetic acid (Trypsin EDTA), and L-Glutamine (200 mM) [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

“…Both oil samples (20 µL) were added to 2 mL of 96% ethanol (Merk Millipore, Burlington, MA, USA), further deposited on a clean glass substrate, and heated at 200 °C for 30 min. The characteristic line scans are displayed below the images in the so-called “enhanced contrast” mode [ 48 ], evidencing the surface profile of both oil samples.…”

Section: Methodsmentioning

confidence: 99%

“…The bacterial and fungal inocula preparation used the direct colony suspension method [ 167 ], as previously shown [ 48 ]. The samples were UBO and CNO in the emulsioned form.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, they are considered important bioactive representatives with pharmaceutical applications [ 37 ] as a potential source of anticancer [ 38 , 39 , 40 ] and antibiotic medicines [ 41 , 42 , 43 ]. Recently, a few authors performed lichens incorporation in different pharmaceutical formulations [ 44 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

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ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study

Popovici

Musuc

Matei

et al. 2022

IJMS

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Oxidative stress is associated with aging, cancers, and numerous metabolic and chronic disorders, and phenolic compounds are well known for their health-promoting role due to their free-radical scavenging activity. These phytochemicals could also exhibit pro-oxidant effects. Due to its bioactive phenolic secondary metabolites, Usnea barbata (L.) Weber ex. F.H. Wigg (U. barbata) displays anticancer and antioxidant activities and has been used as a phytomedicine for thousands of years. The present work aims to analyze the properties of U. barbata extract in canola oil (UBO). The UBO cytotoxicity on oral squamous cell carcinoma (OSCC) CLS-354 cell line and blood cell cultures was explored through complex flow cytometry analyses regarding apoptosis, reactive oxygen species (ROS) levels, the enzymatic activity of caspase 3/7, cell cycle, nuclear shrinkage (NS), autophagy (A), and synthesis of deoxyribonucleic acid (DNA). All these studies were concomitantly performed on canola oil (CNO) to evidence the interaction of lichen metabolites with the constituents of this green solvent used for extraction. The obtained data evidenced that UBO inhibited CLS-354 oral cancer cell proliferation through ROS generation (316.67 × 104), determining higher levels of nuclear shrinkage (40.12%), cell cycle arrest in G0/G1 (92.51%; G0 is the differentiation phase, while during G1 phase occurs preparation for cell division), DNA fragmentation (2.97%), and autophagy (62.98%) than in blood cells. At a substantially higher ROS level in blood cells (5250.00 × 104), the processes that lead to cell death—NS (30.05%), cell cycle arrest in G0/G1 (86.30%), DNA fragmentation (0.72%), and autophagy (39.37%)—are considerably lower than in CLS-354 oral cancer cells. Our work reveals the ROS-mediated anticancer potential of UBO through DNA damage and autophagy. Moreover, the present study suggests that UBO pharmacological potential could result from the synergism between lichen secondary metabolites and canola oil phytoconstituents.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The bacterial and fungal inocula preparation used the direct colony suspension method [ 167 ], as previously shown [ 48 ]. The samples were UBO and CNO in the emulsioned form.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study

Popovici

Musuc

Matei

et al. 2022

IJMS

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“…Their action can be topical [6][7][8] or systemic [9][10][11]. The variety of active ingredients contained in OF is wide: plant extracts [12][13][14][15], herbal compounds [16][17][18], nutraceuticals [19], conventional drugs [20][21][22][23][24][25][26], antibiotics [27], antivirals [28,29], vaccines [30], insulin [31], and other proteins [32].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Printing Technologies in Oral Films Manufacturing—A Minireview

Ozon,

Sarbu,

Popovici

et al. 2023

Processes

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The interest in buccal drug delivery is under consideration due to some distinct properties compared to the traditional pharmaceutical formulations for oral administration: significantly higher bioavailability, a faster absorption rate of the drug, and substantial compliance for special needs patients. Oral films are obtained through various technologies, from conventional tools to 3D and 4D printing approaches. This minireview aims to describe the current additive manufacturing technologies in oral film fabrication, display their advantages and limitations, and discuss various formulation strategies. It also provides advanced data regarding synthetic and natural polymers used in 3D printing technologies for oral films. Moreover, it shows the most recent studies with 3D-printed orodispersible films and mucoadhesive buccal films manufactured through previously analyzed methods. Finally, conclusions and future perspectives are also briefly summarized.

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Implications of the Cultivation of Rosemary and Thyme (Lamiaceae) in Plant Communities for the Development of Antioxidant Therapies

Luță

Biță

Moroșan

et al. 2023

IJMS

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Oxidative stress is the most critical factor in multiple functional disorders’ development, and natural antioxidants could protect the human body against it. Our study aims to investigate the polyphenol content of four extracts of two medicinal plants (Rosmarinus officinalis L. and Thymus vulgaris L.) and analyze the correlation with their antioxidant activity. The research was carried out on extracts of rosemary and thyme obtained from species cultivated together in plant communities. Both were compared with extracts from species cultivated in individual crops (control crops). Their polyphenols were determined by spectrophotometric methods (dosage of flavones, phenol carboxylic acids, and total polyphenols) and chromatography (UHPLC–MS and FT–ICR MS). Triterpenic acids were also quantified, having a higher concentration in the thyme extract from the culture. The antioxidant activity of the dry extracts was evaluated in vitro (DPPH, ABTS, and FRAP) and in silico (prediction of interactions with BACH1/BACH2 transcription factors). The concentrations of polyphenols are higher in the extracts obtained from the sources collected from the common crops. These observations were also validated following the chromatographic analysis for some compounds. Statistically significant differences in the increase in the antioxidant effect were observed for the extracts from the common batches compared to those from the individual ones. Following the Pearson analysis, the IC50 values for each plant extract were strongly correlated with the concentration of active phytoconstituents. Molecular docking studies revealed that quercetin could bind to BTB domains of BACH1 and BACH2 transcription factors, likely translating into increased antioxidant enzyme expression. Future studies must validate the in silico findings and further investigate phytosociological cultivation’s effects.

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Design, Characterization, and Anticancer and Antimicrobial Activities of Mucoadhesive Oral Patches Loaded with Usnea barbata (L.) F. H. Wigg Ethanol Extract F-UBE-HPMC

Cited by 7 publications

References 100 publications

ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study

ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study

Three-Dimensional Printing Technologies in Oral Films Manufacturing—A Minireview

Implications of the Cultivation of Rosemary and Thyme (Lamiaceae) in Plant Communities for the Development of Antioxidant Therapies

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