Design, development, and performance of a fieldable chemical and biological agent detector

Hart, Kevin J.; Wise, M. B.; Griest, W.H.; Lammert, Stephen A.

doi:10.1002/1520-6521(2000)4:2/3<93::aid-fact4>3.0.co;2-w

Cited by 28 publications

(16 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As methods for aerosol analysis continue to evolve, new challenges arise such as the need for rapid on-line identification tools for bioaerosols (197,198). It is possible that single-particle MS instruments, whose major strength lies in their ability to differentiate between sources, may allow for on-line differentiation of spores from other background particles in the environment (199).…”

Section: Discussionmentioning

confidence: 99%

Recent Advances and Some Remaining Challenges in Analytical Chemistry of the Atmosphere

2003

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Recent Advances and Some Remaining Challenges in Analytical Chemistry of the Atmosphere

2003

View full text Add to dashboard Cite

“…One instrument called the "Block II CBMS", uses pyrolysis / methylation to create detectable species from biologicals [29,43]. The system, however, weighs approximately 130 lbs.…”

Section: 122mentioning

confidence: 99%

Miniature Sensors for Biological Warfare Agents using Fatty Acid Profiles: LDRD 10775 Final Report

Mowry¹,

Morgan²,

Frye-Mason³

et al. 2003

View full text Add to dashboard Cite

Rapid detection and identification of bacteria and other pathogens is important for many civilian and military applications. The taxonomic significance, or the ability to differentiate one microorganism from another, using fatty acid content and distribution is well known. For analysis fatty acids are usually converted to fatty acid methyl esters (FAMEs). Bench-top methods are commercially available and recent publications have demonstrated that FAMEs can be obtained from whole bacterial cells in an in situ single-step pyrolysis/methylation analysis.This report documents the progress made during a three year Laboratory Directed Research and Development (LDRD) program funded to investigate the use of microfabricated components (developed for other sensing applications) for the rapid identification of bioorganisms based upon pyrolysis and FAME analysis. Components investigated include a micropyrolyzer, a microGC, and a surface acoustic wave (SAW) array detector. Results demonstrate that the micropyrolyzer can pyrolyze whole cell bacteria samples using only milliwatts of power to produce FAMEs from bacterial samples. The microGC is shown to separate FAMEs of biological interest, and the SAW array is shown to detect volatile FAMEs. Results for each component and their capabilities and limitations are presented and discussed. This project has produced the first published work showing successful pyrolysis/methylation of fatty acids and related analytes using a microfabricated pyrolysis device. 4 AcknowledgmentsThe authors would like to acknowledge the team that provided this project with components, without their work this LDRD would not have existed:

show abstract

“…[52][53][54] Ion trap-based instruments are typically rugged and compact, consuming less power than many other mass analyzers. 53,[55][56][57] Our laboratory has demonstrated ion traps as mass analyzers for single particles.…”

Section: Introductionmentioning

confidence: 99%

Transportable real-time single-particle ion trap mass spectrometer

Harris

Reilly

Whitten

et al. 2005

Review of Scientific Instruments

View full text Add to dashboard Cite

A transportable ion trap mass spectrometer for real-time detection and characterization of individual airborne particles was constructed by minimal modification of a commercial ion trap mass spectrometer. A blank flange was replaced with a flange containing an aerodynamic lens based inlet, light scattering detection optics and ablation/ionization laser optics. Four holes were drilled into the ring electrode. Timing electronics boards running off of in-place power systems were added and integrated with the existing software. The modified mass spectrometer and laser system was packaged in a rugged wheeled frame for easy transport. Particles entered the instrument through a 100μm orifice and were passed through an aerodynamic lens system that produced a well-collimated particle beam over a wide range of sizes. The particle beam passed through a skimmer into the main chamber where individual particles were optically detected and sized with two focused 532nm diode lasers on their way to the ion trap. When the particles reached the center of the trap, they were ablated and ionized with a focused 266nm laser. The nascent ions were then mass analyzed using standard ion trap techniques, including tandem mass spectrometry. Each detected particle was characterized with a mass spectrum and an aerodynamically determined particle size. Careful design minimized the weight and size of the instrument to 104kg and 69×71×76cm, with power consumption less than 1.5kW. Tandem mass spectrometry was demonstrated for identification of ions through collision-induced dissociation (CID) up to mass spectrometry (MS).4 Unit mass resolution was observed in both the parent and CID mass spectra.

show abstract

Design, development, and performance of a fieldable chemical and biological agent detector

Cited by 28 publications

References 12 publications

Recent Advances and Some Remaining Challenges in Analytical Chemistry of the Atmosphere

Recent Advances and Some Remaining Challenges in Analytical Chemistry of the Atmosphere

Miniature Sensors for Biological Warfare Agents using Fatty Acid Profiles: LDRD 10775 Final Report

Transportable real-time single-particle ion trap mass spectrometer

Contact Info

Product

Resources

About