Design, expression, and characterization of a novel dendritic cell-targeted proteins

Wang, Yu; Zhu, Xiao; Wang, Fang; Wu, Shuai; Wang, Zi Cheng; Du, Zhi‐Yan; Yan, Jun; Yu, Ji Yun

doi:10.1016/j.bbrc.2015.03.015

Cited by 6 publications

(8 citation statements)

References 17 publications

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“…6d). 134,135 Apart from the aforementioned techniques, potential effective delivery may also be achieved by administering the combination of nucleic acids with compounds such as lipids and polymers. In recent years, LNPs have become an attractive delivery approach in vaccine development (Fig.…”

Section: Several Promising Delivery Approachesmentioning

confidence: 99%

A systematic review of SARS-CoV-2 vaccine candidates

Dong

Dai

Wei

et al. 2020

Sig Transduct Target Ther

505

489

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging virus that is highly pathogenic and has caused the recent worldwide pandemic officially named coronavirus disease (COVID-19). Currently, considerable efforts have been put into developing effective and safe drugs and vaccines against SARS-CoV-2. Vaccines, such as inactivated vaccines, nucleic acid-based vaccines, and vector vaccines, have already entered clinical trials. In this review, we provide an overview of the experimental and clinical data obtained from recent SARS-CoV-2 vaccines trials, and highlight certain potential safety issues that require consideration when developing vaccines. Furthermore, we summarize several strategies utilized in the development of vaccines against other infectious viruses, such as severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), with the aim of aiding in the design of effective therapeutic approaches against SARS-CoV-2.

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Section: Several Promising Delivery Approachesmentioning

confidence: 99%

A systematic review of SARS-CoV-2 vaccine candidates

Dong

Dai

Wei

et al. 2020

Sig Transduct Target Ther

505

489

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“…DC‐targeted protein msSC was previously studied and preserved in our laboratory. According to our previous research , the complex formation of pSVK‐HBVA/msSC was at an N/P ratio of 20.…”

Section: Methodsmentioning

confidence: 81%

“…In our previous study , we developed a novel method of targeting plasmid DNA to DCs via DEC‐205. We cloned and expressed the recombinant protein msSC and demonstrated that msSC can target plasmid DNA to DCs in vivo , resulting in efficient uptake and expression.…”

Section: Discussionmentioning

confidence: 99%

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Enhanced immunity and antiviral effects of an HBV DNA vaccine delivered by a DC‐targeting protein

Wang

et al. 2016

Journal of Viral Hepatitis

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DNA vaccine targeting delivery to DC represents one effective strategy to improve the immunogenicity of the vaccine. In a previous study, we developed a novel DC-targeting recombinant protein that can deliver plasmid DNA to DCs by an electrostatic coupling effect and can thus improve the uptake efficiency of DCs, improving the expression of plasmid DNA in DCs. In this study, we coupled the protein with the HBV DNA vaccine pSVK-HBVA and investigated whether the immunogenicity and antiviral ability of the vaccine can be improved in HBV transgenic mice. The results show that a stronger specific immune response can be induced in mice after immunization with the coupling vaccine. The HBV DNA copy number and circulating antigen HBsAg in the serum of HBV transgenic mice were significantly decreased. Therefore, this study has demonstrated that the DC-targeting protein has the ability to improve the immunogenicity and the antiviral activity of the HBV DNA vaccine pSVK-HBVA. These findings indicate that this DC-targeting protein can be a potential method for the delivery of DNA vaccines directly to DCs.

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“…Chitosan [50][51][52][53][54][55][56][57] Alginate [58][59][60][61] Dextran [62][63][64][65] Chondroitin sulfate [66,67] Hyaluronic acid (HA) [68,69] Pullulan [70][71][72] Pectin [73,74] + + GRAS [a] Proteins Gelatine [75,76] Albumin [77,78] Listeriolysin O (LLO) [79][80][81] Protamine [82,83] Epsilon poly-llysine (ePLL) [84] DC targeting protein [85,86] Zein [48,87,88] + + GRAS, [a] used in food industry Bio-derived polymers Polyspermine [89] Polyarginine [90,91] Polydopamine (PDA) [92] Polyglutamate (PGA) [93] Poly-lactic acid (PLA) [94,95] Poly(lactic-glycolic acid) (PLGA)…”

Section: Polysaccharidesmentioning

confidence: 99%

Biopolymer‐based Carriers for DNA Vaccine Design

et al. 2021

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She studied chemistry at the University of Zagreb, and completed her PhD at the University of Strathclyde,G lasgow,w orking on DNA detection by advanced spectroscopies, such as SERRS. After postdoc research on DNA nanostructuring and artificial enzyme design at the University of Dortmund,s he was agroup leader at Karlsruhe Institute of Technology before joining Cambridge. Her research focuses on nanostructured biomaterials for use in photocatalysis and targeted drug delivery.

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Design, expression, and characterization of a novel dendritic cell-targeted proteins

Cited by 6 publications

References 17 publications

A systematic review of SARS-CoV-2 vaccine candidates

A systematic review of SARS-CoV-2 vaccine candidates

Enhanced immunity and antiviral effects of an HBV DNA vaccine delivered by a DC‐targeting protein

Biopolymer‐based Carriers for DNA Vaccine Design

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