Design, fabrication, and testing of a microfluidic system for cardiac cell culture.

Nguyen, Mai-Dung

doi:10.18297/etd/1057

Cited by 1 publication

(1 citation statement)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo cardiomyocytes are under constant physical stimulation (pressure, stretch, and shear stress) and mechanical stress during contraction and relaxation. 37 Fluid flow and shear stress influence cell proliferation and phenotype. Cell orientation is an important factor in cardiomyocyte signaling (random cell arrangement does not mimic physiological conditions).…”

Section: Resultsmentioning

confidence: 99%

Heart-on-a-Chip: An Investigation of the Influence of Static and Perfusion Conditions on Cardiac (H9C2) Cell Proliferation, Morphology, and Alignment

et al. 2017

View full text Add to dashboard Cite

Lab-on-a-chip systems are increasingly used as tools for cultures and investigation of cardiac cells. In this article, we present how the geometry of microsystems and microenvironmental conditions (static and perfusion) influence the proliferation, morphology, and alignment of cardiac cells (rat cardiomyoblasts—H9C2). Additionally, studies of cell growth after incubation with verapamil hydrochloride were performed. For this purpose, poly(dimethylsiloxane) (PDMS)/glass microfluidic systems with three different geometries of microchambers (a circular chamber, a longitudinal channel, and three parallel microchannels separated by two rows of micropillars) were prepared. It was found that static conditions did not enhance the growth of H9C2 cells in the microsystems. On the contrary, perfusion conditions had an influence on division, morphology, and the arrangement of the cells. The highest number of cells, their parallel orientation, and their elongated morphology were obtained in the longitudinal microchannel. It showed that this kind of microsystem can be used to understand processes in heart tissue in detail and to test newly developed compounds applied in the treatment of cardiac diseases.

show abstract

Section: Resultsmentioning

confidence: 99%

Heart-on-a-Chip: An Investigation of the Influence of Static and Perfusion Conditions on Cardiac (H9C2) Cell Proliferation, Morphology, and Alignment

et al. 2017

View full text Add to dashboard Cite

show abstract

Design, fabrication, and testing of a microfluidic system for cardiac cell culture.

Abstract: Nguyen, Mai-Dung Thi, "Design, fabrication, and testing of a microfluidic system for cardiac cell culture." (2010).

Cited by 1 publication

References 44 publications

Heart-on-a-Chip: An Investigation of the Influence of Static and Perfusion Conditions on Cardiac (H9C2) Cell Proliferation, Morphology, and Alignment

Heart-on-a-Chip: An Investigation of the Influence of Static and Perfusion Conditions on Cardiac (H9C2) Cell Proliferation, Morphology, and Alignment

Contact Info

Product

Resources

About