This study investigates the use of biometric technology in public transit to improve trip safety and effectiveness. The incorporation of biometric technology into transit networks improves efficiency and security but also poses substantial challenges of privacy, standardisation, and public acceptability. Conducted via a poll of 25 specialists in Lithuania, the objective was to assess the level of acceptability and practicality of using biometric identification for both drivers and passengers. The results suggest a divided view about the specific demographic that biometric applications should target. However, there is agreement on the considerable potential of these technologies to enhance transportation safety. Face recognition has been the favoured approach due to its non-intrusive nature and simplicity of integration. The statistical research demonstrated significant positive correlations between different biometric approaches, indicating that a multimodal strategy is effective for providing full security coverage. The research highlights the significance of resolving privacy issues, emphasising that public acceptability depends on the open management and strong safeguarding of biometric data. The findings support the deliberate use of biometric technologies in sustainable public transportation, emphasising their ability to improve safety, optimise operations, and even revolutionise the passenger experience. This emphasises the equitable examination of technology, security, and privacy in the progress of sustainable public transportation systems. Biometric technology in public transport, especially for monitoring driver health and ensuring passenger safety, is supported by experts as a means to enhance service quality, reduce accidents, and optimize route planning.