A dielectric resonator (DR) based MIMO (Multiple Input Multiple Output) antenna with enhanced isolation is proposed in this letter. The proposed MIMO antenna consists of four hemispherical shaped dielectric resonator (HDR) radiating at 4.9 GHz. The isolation between two consecutive radiators is enhanced by rotating feeding line of one element at an angle of 180 •. The antenna is studied in terms of S-parameters, gain, envelope correlation coefficient (ECC), channel capacity loss (CCL), and diversity gain (DG). All the parameters are found to be within acceptable range. The proposed design is fabricated, and it is found that measured results are in good agreement with simulation.