Design of a probe based on poly(glycidyl methacrylate‐co‐vinylferrocene)‐coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues

Abstract

In this study, a new probe based on immobilization of amino linked oligonucleotide (NH2‐linked DNA) on poly(glycidyl methacrylate‐co‐vinylferrocene)‐coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH2‐linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2‐linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10–100 μg mL−1) with a detection limit (4.7 μg mL−1) and a good selectivity of the NH2‐linked DNA probe toward target DNA sequence. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40638.

A versatile reactive oxygen species‐responsive gels sensor for analysis of metabolic species

“…Common redox functional linkers integrated in responsive polymers include organochalcogen (Se or Te), arylboranes, tetrathiafulvalene, transition metal ions, sulphur‐based moieties (e.g. thioethers, thioketal, vinyldithioether), and ferrocene scaffolds (Bas et al, 2014; Saleem et al, 2015; Senel et al, 2010; Wu et al, 2017). Of these, arylboranes and ferrocene linkers are the most promising due to their ease of integration as functional monomers in polymerisation, high responsivity to ROS, excellent electron transfer, commercial availability, relative environmental stability, low biotoxicity and cost of integration (Bas et al, 2014; Saleem et al, 2015; Senel et al, 2010; Wu et al, 2017).…”

“…thioethers, thioketal, vinyldithioether), and ferrocene scaffolds (Bas et al, 2014; Saleem et al, 2015; Senel et al, 2010; Wu et al, 2017). Of these, arylboranes and ferrocene linkers are the most promising due to their ease of integration as functional monomers in polymerisation, high responsivity to ROS, excellent electron transfer, commercial availability, relative environmental stability, low biotoxicity and cost of integration (Bas et al, 2014; Saleem et al, 2015; Senel et al, 2010; Wu et al, 2017). Improving the redox polymers for responsivity to ROS remains an area of interest.…”

“…The poly (GMA‐co‐ EGDMA‐ co Fc) polymer embodies the appropriate hydrophobic character for lipophilic analytes compatibility. In addition, the pendant epoxy groups of the polymer play the role as immobilisation linkers to the amine groups of enzymes (Bas et al, 2014; Senel et al, 2010). Briefly, the ROS sensor comprises polydimethylsiloxane (PDMS) substrate coated with AgNPs, carbon nanotube/cellulose nanocrystal (CNT/CNC) to create a conductive flexible substrate, which was then coated with the nanoferrogels.…”