Design of aptamer-based sensing platform using gold nanoparticles functionalized reduced graphene oxide for ultrasensitive detection of Hepatitis B virus

Mohsin, Dheyaa Hussein; Mashkour, Muthana Saleh; Fatemi, Fataneh

doi:10.1007/s11696-020-01292-1

Cited by 28 publications

(18 citation statements)

References 75 publications

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“…Currently, several aptamer-based sensors have been developed to detect HBsAg, including chemiluminescent [ 76 , 77 ], fluorescent [ 41 ], electrochemical aptasensors [ 78 ], etc. Cho and coworkers [ 79 ] reported a new nanomaterial-based FET platform for the non-invasive detection of HBsAg.…”

Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

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Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

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“…After the aptamer was successfully immobilized on the surface of the SPCE/AuNP electrode with the streptavidin-biotin system, the non-specific binding site on the electrode surface was incubated using 1 % BSA solution for 15 minutes at 25 °C, and then rinsed with PBS solution pH 7.4 0.01 M. After that, a solution of RBD protein S SARS-CoV-2 with a certain concentration was dropped on the electrode and incubated for 60 minutes at 25 °C. Then, using a redox system of 10 mM K3[Fe(CN)6] solution in 0.1 M KCl, differential pulse voltammetry was performed over a potential range of -1.0 to 1.0 V at a scan rate of 0.008 V/s, Estep 0.004 V with an Epulse of 0.025 V and tpulse of 0.05 s [22].…”

Section: Fabrication Of the Electrochemical Aptasensormentioning

confidence: 99%

“…Factors such as aptamer concentration (X1), aptamer incubation time (X2), and incubation time of RBD protein S SARS-CoV-2 (X3) were selected as factors to be optimized in the experiment [22,38]. Each factor is designed at three different levels, the lowest (-1), medium (0), and highest (+1) levels, as shown in Table 1.…”

Section: Determination Of Optimum Conditions From Parameters Affectin...mentioning

confidence: 99%

“…The aptamer is a single-stranded DNA or RNA nucleic acid molecule selected through an in vitro method known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX) [22], which can bind to various targets with high selectivity and specificity, such as metal ions, small molecules, proteins, amino acids, peptides, microorganisms, cells, viruses, and other nucleic acids [23][24][25]. Compared with antibodies, aptamers have several advantages, namely smaller size, thermal stability, high affinity, excellent sensitivity, easy synthesis, lower toxicity, and easy chemical modification [22]. Biosensors that utilize aptamers as identification elements are referred to as aptasensors.…”

Section: Introductionmentioning

confidence: 99%

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The optimization of an electrochemical aptasensor to detect RBD protein S SARS-CoV-2 as a biomarker of COVID-19 using screen-printed carbon electrode/AuNP

Sari

Hartati

Gaffar

et al. 2022

J. Electrochem. Sci. Eng.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus identified as the cause of the Coronavirus Disease 2019 (COVID-19) outbreak. The gold standard for detecting this virus is polymerase chain reaction (PCR). The electrochemical biosensor method can be an alternative method for detecting several biomolecules, such as viruses, because it is proven to have several advantages, including portability, good sensitivity, high specificity, fast response, and ease of use. This study aims to optimize an electrochemical aptasensor using an AuNP-modified screen-printed carbon electrode (SPCE) with an aptamer to detect RBD protein S SARS-CoV-2. Aptasensors with the streptavidin-biotin system were immobilized on the SPCE/AuNP surface via covalent bonding with linkers to 3-mercaptopropionic acid (MPA) and electrochemically characterized by the [Fe(CN)6]3-/4- redox system using differential pulse voltammetry. The results showed that the immobilized aptamer on the SPCE/AuNP electrode surface experienced a decrease in current from 11.388 to 4.623 µA. The experimental conditions were optimized using the Box-Behnken experimental design for the three factors that affect the current response. The results of the optimization of the three parameters are the concentration of aptamer, incubation time of aptamer, and incubation time of RBD protein S SARS-CoV-2, each of which is 0.5 µg/mL, 40 minutes, and 60 minutes, respectively. The RBD protein S SARS-CoV-2 with various concentrations was tested on an electrochemical aptasensor to determine the detection limit and quantification limit, and the respective results were 2.63 and 7.97 ng/mL. The electrochemical aptasensor that has been developed in this study can be applied to detect RBD protein S SARS-CoV-2 as a COVID-19 biomarker in a simple, practical, and sensitive way.

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“…Also, aptamers are cheaper and can be synthesized with several functional groups [ 7 ]. Up to now, several electrochemical aptasensors have been designed for the diagonalization of viruses such as hepatitis B, [ 11 , 12 ], hepatitis C [ 13 ], virion [ 14 ], murine norovirus [ 15 ], human immunodeficiency virus (HIV) [ 16 ] influenza H1N1 [ 17 , 18 ], and influenza H5N1 [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%