Design of artificial nucleases and studies of their interaction with DNA

Zhang, Jingjing; Shao, Ying; Li, Wei; Liu, Ying; Sheng, Xin; Liu, Fang; Lu, Guoyuan

doi:10.1007/s11426-009-0029-8

Cited by 19 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An effective way to achieve these goals is to complex the drug molecule with a host carrier molecule or receptor stabilized by intermolecular interactions [3]. Some well-known examples include cyclodextrins (CDs) and crown ethers, which have been extensively explored as drug carriers to decrease the toxicity and enhance the solubility, stability and bioavailability of drug molecules [4,5]. Q[n]s are another example of interesting compounds having the potential to be drug carriers.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of inclusion complexes of antitumor camptothecin with cucurbit[n = 7, 8]urils

Dong

Zhao

et al. 2010

Sci. China Chem.

View full text Add to dashboard Cite

The slightly water-soluble anticancer drug camptothecin (CPT) and its inclusion complexes with cucurbit[n = 7, 8]uril (Q[n] (n = 7, 8)) were investigated. The formation of 1:2 complexes with Q[n] (n = 7, 8) in aqueous solution was confirmed by fluorescence spectroscopy and the apparent stability constants were determined to be higher than 3.01 × 10 12 L 2 /mol 2 . The solid inclusion complexes of CPT and Q[n] (n = 7, 8) were also prepared by the co-evaporation method and characterized by Fourier transformation-infrared spectroscopy, differential scanning calorimetry and powder X-ray diffraction. Aqueous solubility and dissolution studies indicate that the complexes exhibited significantly increased dissolution rates compared with the pure drug and physical mixtures. The potential of Q[7] or Q[8] for stabilizing lactone modality of CPT was investigated by the High Performance Liquid Chromatography (HPLC) method. The results reveal more than 63% CPT lactone form (active form) in or Q[8] complexes compared to only 36% CPT lactone form in the absence of Q[7] or Q[8] after being incubated in the phosphate buffer solution (pH 7.4 at 37 °C) for 5 h. complexes, camptothecin, cucurbit[n]uril (n = 7, 8), dissolution properties, stability

show abstract

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of inclusion complexes of antitumor camptothecin with cucurbit[n = 7, 8]urils

Dong

Zhao

et al. 2010

Sci. China Chem.

View full text Add to dashboard Cite

show abstract

“…Ragunathan and Schneider [36] found that when Pr(III), Eu(III), etc. are coordinated with ligands 28 and 29 containing pyridine rings to form dinuclear complexes M(III)-28 and M(III)-29 (see Figure 6) 50 min, whose cleavage rate is 2.1 × 10 7 times faster than that of uncatalysed DNA cleavage. Although lanthanide metal ion complexes can catalyse the hydrolytic cleavage of nucleic acids effectively, they have the disadvantage that these metal ions are not bio-elements per se, therefore, such systems are cytotoxic, and can be used only in vitro [7].…”

Section: Multinuclear Metal Complexes Of Aza-crown Ethers Used As Mimmentioning

confidence: 99%

Nucleic Acid and Phosphoester Hydrolytic Cleavage Catalysed by Aza-Crown Ether Metal Complexes as Synthetic Nucleases

Fěng

Lan

et al. 2014

Progress in Reaction Kinetics and Mechanism

View full text Add to dashboard Cite

Aza-crown ethers are ligands in which the oxygen atoms are replaced by nitrogen atoms in the crown ether ring systems. This type of ligand possesses specific complexation with metal ions, such as those of transition-metals, rare earths, alkali metals and alkaline earths, which form metal complexes whose structures are similar to those of some biological enzymes. In recent decades, research on aza-crown ethers and their metal complexes as mimics of nucleases in hydrolysing nucleic acids has attracted increasing attention. These studies illuminate the mechanism of nucleic acid hydrolytic cleavage as catalysed by natural nucleases. In order to assist the design and synthesis of highly active, selective and stable mimic nucleases, this paper reviews recent progress in the investigation of aza-crown ether metal complexes as mimic nucleases, including: the relationship between the structures and activities of synthetic metallonucleases; multicentre synergistic catalysis of metal ions in multinuclear complexes; bifunctional cooperative catalysis of the branches and ions in the complexes; and especially, the structural characteristic and catalytic mechanism of aza-crown ether metal complexes as mimic nucleases. 210 complexation with many metal ions such as those of transition metals, rare earths, alkali metals and alkaline earths [1 -4]. Aza-crown ethers can not only coordinate with positively charged ions, but can also cooperate with negatively charged anions. Furthermore, because of the special structure of aza-crown ethers, some aza-crown ether metal complexes possessing a specific structure can be obtained by chemical modification, which can be used as enzyme models to mimic the recognition process of enzymic catalytic reaction, which thus provide many possible ways to study life functions [5,6]. Accordingly, these complexes have significant potential application in some aspects including biological medicine, biomimetic chemistry, cell membrane transmission, mimic metalloenzymes and supramolecular assembly. A nucleic acid is a long-chain polynucleotide, which falls into two categories: one is desoxyribonucleic (DNA), which is used as the carrier of hereditary information for storing, copying and transferring; the other is ribonucleic acid (RNA), which is the origin of protein synthesis. The synthesis and degradation of nucleic acids both depend on the catalysis of enzymes in organisms [7]. The application of natural enzymes in many aspects like medical treatment, pharmacy, environment protection, is hampered because of their disadvantages such as poor stability, difficult in separation and purification, and high cost. It is urgent to mimic the structure of natural nucleases, in the design and synthesis of chemical nucleases possessing high catalytic activity and specific recognition just like natural nucleases [8]. Chemical nucleases are a kind of functional complex of small molecules or macromolecules that have the catalytic active centre of nucleases and a simple structure compared to natural enzymes, which should ...

show abstract

“…It is a valuable and significant work for vitro selection of drugs, diagnosis and treatment of some diseases as well as the design and synthesis of novel anti-cancer. In the clinical field many anti-cancer drugs were used DNA as the main targets, which interacted with the cancer cells in DNA and damaged its structure, thereby affecting gene automatic regulation and expression, showing anti-cancer activity [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Study on Resonance Rayleigh Scattering Spectra of Mercaptopurine-Cu(II)-Nucleic Acid System

Yang¹,

Yang²

2012

AJAC

View full text Add to dashboard Cite

In pH 4.8 Britton-Robinson (B-R) buffer solution, Mercaptopurine (MP) could react with Cu(II) to form stable chelate compound, a new Resonance Rayleigh scattering (RRS) spectrum generated and enhanced for the binary system which the highest peak located at 453 nm as detection wavelength. But the RRS spectra could be quenched after adding herring milt DNA (hsDNA), salmon milt DNA (sDNA) and calf thymus DNA (ctDNA) into the binary system, respectively. And the weakened degree of spectra was directly proportional to the concentration of DNA. The reaction product of three nucleic acid system have identical spectral features, their range of linearity for the relation of spectral intensity with concentration respectively are 0.05 -0.9 μg·mL -1 for hsDNA, 0.1 -0.9 μg·mL -1 for sDNA and 0.3 -0.9 μg·mL -1 for ctDNA; their detection limit respectively are 5 ng·mL -1 for hsDNA, 6 ng·mL -1 for sDNA, 6 ng·mL -1 for ctDNA. So a new method for determination of DNA was developed and successfully applied to determine the content of the DNA in artificial synthetized samples. At the same time the spectral features of absorption spectra and RRS spectra of the three reaction system, and the eligible reaction conditions and influencing factors were investigated in this paper.

show abstract

Design of artificial nucleases and studies of their interaction with DNA

Cited by 19 publications

References 42 publications

Preparation and characterization of inclusion complexes of antitumor camptothecin with cucurbit[n = 7, 8]urils

Preparation and characterization of inclusion complexes of antitumor camptothecin with cucurbit[n = 7, 8]urils

Nucleic Acid and Phosphoester Hydrolytic Cleavage Catalysed by Aza-Crown Ether Metal Complexes as Synthetic Nucleases

Study on Resonance Rayleigh Scattering Spectra of Mercaptopurine-Cu(II)-Nucleic Acid System

Contact Info

Product

Resources

About