Recently, the best antenna structures have considered microstrip patch antenna due to their simple construction, low cost, minimum weight, and the fact that they can be effortlessly integrated with circuits. To achieve multiband operation an antenna is designed with an etching rectangle and circle slot on the surface of the patch to achieve multi-band frequency capabilities in mid-band 5G applications. Inset-fed structure type of fed of all antenna printed and fabricated on the brow of the Rogers RT5880 substrate. Then, prototype structures of the microstrip patch antenna were acquired during the design process until achieving the desired antennas. The antenna_1 achieved tri-band characteristics covering the WiMAX band including 2.51 -2.55 GHz, WLAN, and S-band including 3.80 -3.87 GHz and C-and X-band including 6.19 -6.60 GHz. The antenna_2 gives dual-band characteristics covering C-band and X-band including (6.72 -7.92 GHz) with a peak under -45 dB suitable for mid-band 5G applications. High impedance bandwidth increases between (70 MHz-1.25 GHz) for wireless applications. The proposed microstrip patch antennas were simulated using CST MWS-2015 and were experimentally tested to verify the fundamental characteristics of the proposed design, it offers multiple-band operation with high stable gain and good directional radiation characteristics results.