The objective of E-shaped patch antenna with hexagonal slot is to operate in the ISM band for different kind of applications, such as WLAN, GPS, and various modern wireless systems. The posit antenna is designed using FR4 substrate having a dielectric constant of 4.4 with a thickness of 1.6 mm. Probe feed technique is used for this antenna design. A parametric study was included to determine the effect of design approaches and the antenna performance. The realization of the designed antenna was analyzed in term of boost (gain), return loss, and radiation pattern. The design was upsurged to confirm the best achievable result. This antenna resonates at three different frequencies at 1.6 GHz, 3.24 GHz, and 5.6 GHz with a reflection coefficient less than -10 dB and VSWR<2.