Design, optimization, and application of a conventional PCR assay with an internal control for detection of ‘<i>Candidatus</i>Mycoplasma turicensis’ 16S rDNA in domestic cats from Brazil

Santos, Andrea Pires dos; Messick, Joanne B.; Biondo, Alexander Welker; Oliveira, Simone Tostes de; Pedralli, Viviane; Lasta, Camila Serina; Lacerda, Luciana A.; Esteves, Vanessa; Hofmann‐Lehmann, Regina; Willi, Barbara; González, Félix Hilario Díaz

doi:10.1111/j.1939-165x.2009.00158.x

Cited by 29 publications

(42 citation statements)

References 34 publications

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“…Although hemoplasma infection has been documented in cats in several regions of Brazil (DE MORAIS et al, 2007;MACIEIRA et al, 2008;SANTOS et al, 2009;BRAGA et al, 2012;DE BORTOLI et al, 2012;MICELI et al, 2013), few reports clarifying actual epidemiology have yet been published and São Paulo (ANDRÉ et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Besides, Santos et al (2009) described a conventional PCR aiming to amplify a 16S rRNA fragment of 'Candidatus M. turicensis'. When we aligned primers designed by Criado-Fornelio et al (2003) to 16S rRNA 'Candidatus M. turicensis' sequence, a 595 pb fragment can be amplified, similar to that found for M. haemofelis and 'Candidatus M. haemominutum' (600 pb).…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Santis

Herrera

Sousa

et al. 2014

Rev. Bras. Parasitol. Vet.

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Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis and 15 (9.9%) for 'Candidatus M. turicensis', from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Santis

Herrera

Sousa

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…Partial sequences of the 16S rRNA gene of M. haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis' were amplified by means of PCR in final-volume reaction mixtures of 25 µL containing 5 µL of template DNA, 10X (2.5 µL) PCR buffer, 1.0 mM (1 µL) MgCl 2 , 0.2 mM (2 µL) deoxynucleotide triphosphate (dNTP) mixture, 1.5 U (0.25 µL) Taq DNA polymerase (Invitrogen, Carlsbad, California, USA) and 0.2 mM (1 µL) of primers that had previously been described (CRIADO-FORNELIO et al, 2003;SANTOS et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

“…In Brazil, the prevalences of hemoplasmas among healthy and anemic cats have been found to range from 2.1% to 38% for M. haemofelis; 4% to 13.5% for 'Candidatus M. haemominutum'; and 0.37% to 2.7% for 'Candidatus M. turicensis' (BATISTA, 2004;MACIEIRA et al, 2008;HORA, 2008;SANTOS et al, 2009;BRAGA et al, 2012;DE BORTOLI et al, 2012). DNA from Bartonella henselae and B. clarridgeiae was detected in three and one out of the 178 cats sampled (1.7%), respectively.…”

Section: Mycoplasma Haemofelismentioning

confidence: 99%

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Miceli

Gavioli

Gonçalves

et al. 2013

Rev. Bras. Parasitol. Vet.

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Hemotrophic mycoplasmas (hemoplasmas), Bartonella sp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas, Bartonella sp., Hepatozoon sp. and Cytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) for Mycoplasma haemofelis, 12 (6.7%) for 'Candidatus M. haemominutum' and one (0.5%) for 'Candidatus M. turicensis'. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, 'Candidatus M. haemominutum' and 'Candidatus M. turicensis', based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showed Cytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas and Bartonella species.

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“…No Brasil, os hemoplasmas felinos já foram identificados, molecularmente, em amostras de gatos domésticos dos Estados do Paraná, Rio de Janeiro e Rio Grande do Sul (MORAIS et al, 2007;MACIEIRA et al, 2008;SANTOS et al, 2009). Esse grupo de parasitos também foi identificado em felídeos selvagens mantidos em cativeiro nas cidades de Curitiba e de São Paulo, (GUIMARÃES et al, 2007a;WILLI et al, 2007b).…”

Section: Introductionunclassified

Uso da técnica de Southern Blot/HibridizaÇão associada à reaÇão em cadeia da polimerase para aumentar a sensibilidade no diagnóstico das infecÇões por hemoplasmas em gatos domésticos

Macieira¹,

Menezes²,

Damico³

et al. 2009

RBPV

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Design, optimization, and application of a conventional PCR assay with an internal control for detection of ‘CandidatusMycoplasma turicensis’ 16S rDNA in domestic cats from Brazil

Abstract: These results demonstrate the utility of an optimized PCR assay to detect CMtc in feline blood samples. We also report for the first time the prevalence of CMtc infection in domestic cats in Brazil.

Cited by 29 publications

References 34 publications

Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

Uso da técnica de Southern Blot/HibridizaÇão associada à reaÇão em cadeia da polimerase para aumentar a sensibilidade no diagnóstico das infecÇões por hemoplasmas em gatos domésticos

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