Designation of the Provisional New Enterococcus Species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., Isolated from Human Blood, and Identification of a Strain Previously Named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004

Abstract

We have previously characterized two new enterococcal species (provisionally designated CDC PNS-E1 and CDC PNS-E2) recovered from clinically significant specimens associated with invasive infections in humans. In the present report we provide additional data and propose formal denominations for isolates of these two species of Enterococcus . Results of 16S rRNA gene sequencing, sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of whole-cell protein profiles, and DNA-DNA reassociation experiments indicated that the blood isolate ATCC BAA-780 (SS 1728; CDC PNS-E1) corresponds to Enterococcus italicus , whose species epithet was proposed to designate isolates from artisanal Italian cheese. Strain ATCC BAA-781 (CCUG 47861; SS 1729; CDC PNS-E2), a vancomycin-resistant isolate recovered from the blood of a patient in the United States, was found to be highly related at the species level to another blood isolate (SS 1743; CCUG 47884) from Sweden, and for these we propose the designation Enterococcus sanguinicola sp. nov.

Reevaluation of the Taxonomic Status of Recently Described Species of Enterococcus: Evidence that E. thailandicus Is a Senior Subjective Synonym of “E. sanguinicola” and Confirmation of E. caccae as a Species Distinct from E. silesiacus

“…Formal species designation was not proposed at that time in deference to the recommendation in minute 10 of the July 2002 meeting of the International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Staphylococci and Streptococci (15), which refers to the description of new species based on a single isolate. Subsequently an additional strain was identified, and the epithet "Enterococcus sanguinicola" was proposed to accommodate these two strains recovered from clinically relevant human sources (2). This denomination was effectively but not validly published.…”

“…According to the rules of nomenclature, since E. thailandicus is the earliest formally published and valid epithet (10), it should be considered the senior subjective synonym of E. sanguinicola and must be used to name this taxon. Therefore, E. thailandicus should include strains previously described as CDC PNS-E2 (3) and later designated E. sanguinicola (2). Clinical laboratories should be aware on the occurrence of this additional enterococcal species potentially associated with vanA gene acquisition and transmission.…”

“…Two E. sanguinicola strains, ATCC BAA-781 T and CCUG 47884, isolated from blood cultures from two patients (2,3), and E. caccae ATCC BAA-1240 T , recovered from a stool sample obtained from a healthy individual (4), were obtained from the CDC Streptococcus Laboratory culture collection (Atlanta, GA). E. thailandicus (NRBC 101867 T ) isolated from fermented sausage (10) was obtained from the NITE Biological Research Center (NBRC) culture collection (Japan), and E. silesiacus (LMG 23085 T ), isolated from drinking water (9), was obtained from the BCCM/LMG bacterial collection (Belgium).…”

The maintenance of the list of QPS microorganisms intentionally added to food or feed - Scientific Opinion of the Panel on Biological Hazards

“…An example is the case of E. italicus, a species described for the first time by Fortina et al (2004), and frequently detected in cheese (Fornasari et al, 2008). This organism, which lacks known virulence factors and is presumed to be a safe species of enterococci (Fortina et al, 2008), is indistinguishable from the newly proposed species E. sanguinicola, identified as being responsible for invasive infection in humans (Carvalho et al, 2008).…”

Isolamento e identificação de Lactobacillus spp., Bifidobacterium spp., Enterococcus spp., Pediococcus spp. e Lactococcus spp. da microbiota intestinal de Papagaio-verdadeiro (Amazona aestiva)

“…Enterococcus CDC PNS E-1, E. CDC PNS E-2 e E. italicus de aves de vida livre e de cativeiro, constituindo-se a primeira descrição destas espécies em psitacídeos. Estas espécies foram recentemente descritas a partir de sangue humano Carvalho et al (2008). evidenciaram que E. italicus correspondia ao E. CDC PNS E-1 e propuseram que E. CDC PNS E-2 fosse denominado de Enterococcus sanguinicola sp.…”