Designing biomaterials based on biomineralization of bone

Alves, Natália M.; Leonor, Isabel B.; Azevedo, Helena S.; Reis, Rui L.; Mano, João F.

doi:10.1039/b910960a

Cited by 160 publications

(129 citation statements)

References 91 publications

(179 reference statements)

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“…36) In particular, the presence of CO 3 2¹ groups had been proved to induce the precipitation of nanocrystals of HCA from SBF in biomimetic methods. 37), 38) In order to evaluate the cytocompatibility of the 1393 glassCNTs nanocomposite scaffolds, an osteoblast proliferation study using MG-63 bone-forming cells after 10 days was cultured on the monolithic 1393 glass and the 1393 glass-3 wt.% CNTs nanocomposite scaffolds (Fig. 6).…”

Section: Resultsmentioning

confidence: 99%

A bioactive glass nanocomposite scaffold toughed by multi-wall carbon nanotubes for tissue engineering

Liu

Gao

Feng

et al. 2015

J. Ceram. Soc. Japan

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Bioactive glasses have wide application prospects in bone replacement and regeneration owing to the unique osteoconductivity and osteostimulativity. However, the high brittleness and poor compressive strength limit their applications in load-bearing positions. In this study, multi-wall carbon nanotubes (CNTs) were used to toughen bioactive glass (1393 glass), and their nanocomposite scaffolds were fabricated by selective laser sintering. The effect of CNTs amount (15 wt.%) on mechanical properties of the scaffolds was investigated. The results showed that CNTs were effective to improve the mechanical properties of the nanocomposite scaffolds by virtue of the toughening mechanisms of bridging, pull-out and crack deflection. The optimum compressive strength and fracture toughness reached to 37.32 MPa and 1.58 MPa·m 1/2 , respectively, by adding the appropriate amount of CNTs (3 wt.%). In addition, the bone-like hydroxycarbonate apatite (HCA) layer was formed on the surface of the nanocomposite scaffolds after immersion in simulated body fluid (SBF) for 10 days. And the cell culture test showed that the scaffolds should have good cytocompatibility. The research indicated that the 1393 glass-CNTs nanocomposites scaffolds are promising candidates for bone tissue engineering applications.

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Section: Resultsmentioning

confidence: 99%

A bioactive glass nanocomposite scaffold toughed by multi-wall carbon nanotubes for tissue engineering

Liu

Gao

Feng

et al. 2015

J. Ceram. Soc. Japan

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“…The SEM images of MTA pastes, immersed for 14 days in SBF (Figure 12), shows the presence of a thin friable layer formed by agglomerations of plate like crystals; this morphology is associated to HAp [27][28][29]. The SEM images of MTA pastes, immersed for 14 days in SBF (Figure 12), shows the presence of a thin friable layer formed by agglomerations of plate like crystals; this morphology is associated to HAp [27][28][29]. The FT-IR analysis (Figure 11) of MTA pastes immersed for 14 days in SBF shows the following specific bands [24][25][26]:…”

Section: Figurementioning

confidence: 99%

“…The SEM images of MTA pastes, immersed for 14 days in SBF (Figure 12), shows the presence of a thin friable layer formed by agglomerations of plate like crystals; this morphology is associated to HAp [27][28][29]. The cytotoxicity of MTA was verified by a MTT assay that is based on biochemical reactions that measure the metabolic activity of living cells.…”

Section: Figurementioning

confidence: 99%

Influence of Thermal Treatment Conditions on the Properties of Dental Silicate Cements

et al. 2016

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Abstract:In this study the sol-gel process was used to synthesize a precursor mixture for the preparation of silicate cement, also called mineral trioxide aggregate (MTA) cement. This mixture was thermally treated under two different conditions (1400˝C/2 h and 1450˝C/3 h) followed by rapid cooling in air. The resulted material (clinker) was ground for one hour in a laboratory planetary mill (v = 150 rot/min), in order to obtain the MTA cements. The setting time and mechanical properties, in vitro induction of apatite formation by soaking in simulated body fluid (SBF) and cytocompatibility of the MTA cements were assessed in this study. The hardening processes, nature of the reaction products and the microstructural characteristics were also investigated. The anhydrous and hydrated cements were characterized by different techniques e.g., X-ray diffraction (XRD), scanning electron microscopy (SEM), infrared spectroscopy (FT-IR) and thermal analysis (DTA-DTG-TG). The setting time of the MTA cement obtained by thermal treatment at 1400˝C/2 h (MTA1) was 55 min and 15 min for the MTA cement obtained at 1450˝C/3 h (MTA2). The compressive strength values were 18.5 MPa (MTA1) and 22.9 MPa (MTA2). Both MTA cements showed good bioactivity (assessed by an in vitro test), good cytocompatibility and stimulatory effect on the proliferation of cells.

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“…However, the migration of the nano HAP particles from the implanted site into surrounding tissues might cause damage to healthy tissues. Current advances in molecular biomimetics suggest that a biomineral-inspired approach may be of value in new classes of biomaterials [3,4]. This approach is based primarily on the idea of macromolecules as templates to control inorganic crystal formation, and seeks to reproduce the nanoscopic and hierarchical structures of natural bone through biological principles and the processes of self-assembly or self-organization [5].…”

Section: Introductionmentioning

confidence: 99%

Rapid biomimetic mineralization of chitosan scaffolds with a precursor sacrificed method in ethanol/water mixed solution

Li¹,

Zhao²,

Ding³

et al. 2011

Express Polym. Lett.

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Abstract. Biomimetic mineralization was performed on a large scale by a rapid and efficient approach. Chitosan scaffolds were placed in a mixed solution of urea, ethanol and distilled water, followed by the introduction of dibasic sodium phosphate (0.1M) and calcium chloride (0.1M) with the molar ratio of 1.67. These mixed solvents was then adjusted to weakly alkaline by adding sodium hydroxide solution. Finally the reaction mixture was sealed and kept at 80ºC for predetermined time. The composition and morphology of the apatite and the hybrid scaffolds were investigated by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier Transform Infrared spectroscopy (FTIR) and environmental scanning electron microscopy (ESEM). The mechanism of nucleation and growth of crystals was discussed as well. The results revealed that chitosan scaffolds improved the crystallinity of hydroxyapatite (HAP) crystals. With the extension of mineralization time, the mineral layers on the outer surface and inner section of chitosan scaffolds increased as well. Furthermore, the compressive strength and modulus of the HAP-chitosan scaffolds biocomposites increased to 0.55±0.003 and 29.29±1.25 MPa respectively. Such one-pot approach may be extended to the mineralization of other materials and will have a broad application in the future.

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Designing biomaterials based on biomineralization of bone

Cited by 160 publications

References 91 publications

A bioactive glass nanocomposite scaffold toughed by multi-wall carbon nanotubes for tissue engineering

A bioactive glass nanocomposite scaffold toughed by multi-wall carbon nanotubes for tissue engineering

Influence of Thermal Treatment Conditions on the Properties of Dental Silicate Cements

Rapid biomimetic mineralization of chitosan scaffolds with a precursor sacrificed method in ethanol/water mixed solution

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