Desirability function for optimization of the synthesis of high-panose isomaltooligosaccharides from maltose catalyzed by a novel commercial enzyme preparation from Aspergillus niger

Recent Advances on Emerging Carbohydrates‐Based Prebiotics and its Potential Food Sources: Marine Algae, Seaweeds, Tropical Fruits, and Agri‐Food Wastes

“…They can contain up to 12 glucosyl units and thus present different degrees of polymerization (DPs), including maltose and isomaltose (DP2), panose, isopanose, and isomaltotriose (DP3), isomaltotetraose (DP4), isomaltopentaose (DP5), long chain and cyclo-IMO (Figure 4b). [30,31] Currently, IMO are already considered GRAS, supporting extensive use of IMO as safe and functional food ingredients. [32] Most commercial IMO are enzymatically manufactured using a substrate containing starch or dextran.…”

“…However, it may lead to substrate inhibition and productivity decrease. [30] Thus, academic and industrial organizations have focused on screening for the novel, economical, and efficient enzymes as the current market of IMO applications is increasing. [9] Immobilized dextranase and dextransucrase used as biocatalysts of sucrose, for example, enable the application of continuous processes and the recovery of the enzyme after conversion.…”

“…Recombinant enzymes such as the 𝛼-glucosidases obtained from Aspergillus niger have also improved IMO production, mainly due to its high levels of transglycosylation activity and region-specific transglycosytic synthesis of IMO using starch as a substrate. [30,32] Increasing maltose conversion into IMO with enzyme membrane reactors and large-scale extraction from cheap and abundant agri-food residues (potato processing waste and banana flour) are other investigated alternatives. [9] From a biological point of view, the different structures of IMO influence the degree to which the compound passes through the colon to be fermented by microorganisms and the healthpromoting effects.…”

Dairy Wastewater Treatment with Organic Coagulants: A Comparison of Factorial Designs

“…This technique consists of converting each response variable into an individual desirability function (d) that varies in the range of 0-1.0. A value of 0 is assigned to predicted response variable values outside the region of interest, and a desirability value of 1.0 corresponds to the response variable when it is at its target [63]. Once the desirability functions (d) were specified for response removed turbidity and COD, these functions were incorporated into a single function called global desirability (D), which is normally obtained by the geometric mean of all individual desirability.…”

Synthesis of Isomaltooligosaccharides (IMOs) from Sweet Potato Starch by Simultaneous Saccharification and Transglycosylation Using Saccharomyces cerevisiae Var. diastaticus BE 134 to Improve Purity of IMOs

“…e recent studies reported that the main constituents found in commercial IMOs consisted of isomaltose (IG 2 ), panose, isomaltotriose (IG 3 ), and isomaltotetraose (IG 4 ) [7]. is may be related to the ability of transglucosidase enzyme derived from Aspergillus niger mainly existent on short-chain oligosaccharide substrates [1,8]. Specifically, Niu et al [4] calculated the formed IMOs concentration synthesizing from enzyme cocktail based on obtained sum of concentrations of all IG 2 , IG 3 , and panose.…”