Desorption/ionization induced by neutral cluster impact as a soft and efficient ionization source for ion trap mass spectrometry of biomolecules

Baur, Markus; Gebhardt, Christoph; Dürr, Michael

doi:10.1002/rcm.6781

Cited by 25 publications

(48 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Induced by Neutral Clusters (DINeC) 36,37 was used as a soft desorption/ionization method for analysing the adsorbed molecules. In short, neutral SO 2 clusters (103 to 104 molecules in size) which are seeded in a helium beam hit the surface and softly desorb and ionize some of the adsorbates.…”

Section: Desorption/ionizationmentioning

confidence: 99%

Electron microscopy of polyoxometalate ions on graphene by electrospray ion beam deposition

et al. 2018

View full text Add to dashboard Cite

Aberration-corrected high-resolution transmission electron microscopy (AC-HRTEM) has enabled atomically resolved imaging of molecules adsorbed on low-dimensional materials like carbon nanotubes, graphene oxide and few-layer-graphene. However, conventional methods for depositing molecules onto such supports lack selectivity and specificity. Here, we describe the chemically selective preparation and deposition of molecules-like polyoxometalate (POM) anions [PWO] using electrospray ion-beam deposition (ES-IBD) along with high-resolution TEM imaging. This approach provides access to sub-monolayer coatings of intact molecules on freestanding graphene, which enables their atomically resolved ex situ characterization by low-voltage AC-HRTEM. The capability to tune the deposition parameters in either soft or reactive landing mode, combined with the well-defined high-vacuum deposition conditions, renders the ES-IBD based method advantageous over alternative methods such as drop-casting. Furthermore, it might be expanded towards depositing and imaging large and nonvolatile molecules with complex structures.

show abstract

Section: Desorption/ionizationmentioning

confidence: 99%

Electron microscopy of polyoxometalate ions on graphene by electrospray ion beam deposition

et al. 2018

View full text Add to dashboard Cite

show abstract

“…As an alternative, we have shown desorption/ionization induced by neutral clusters (DINeC) to be a soft and matrix-free ionization method which has been successfully employed for mass spectrometric analysis of complex molecules . As a consequence of these various aspects, cluster-induced desorption of complex molecules such as peptides, proteins, lipids, and dyes proceeds without any fragmentation of the desorbing molecules 11,15 ; typical mass spectra show the dominant peak at the m/z value of the intact molecule ([M+H] + or [M-H] -, Figure 3). Depending on the number and nature of functional groups in the molecule, multiple charged cations of the form [M + n•H] n+ are observed 11,15,18 .…”

Section: Introductionmentioning

confidence: 99%

“…As a consequence of these various aspects, cluster-induced desorption of complex molecules such as peptides, proteins, lipids, and dyes proceeds without any fragmentation of the desorbing molecules 11,15 ; typical mass spectra show the dominant peak at the m/z value of the intact molecule ([M+H] + or [M-H] -, Figure 3). Depending on the number and nature of functional groups in the molecule, multiple charged cations of the form [M + n•H] n+ are observed 11,15,18 . For biomolecules, ionization typically takes place via uptake or abstraction of a proton at a basic or acidic functional group, respectively 11 .…”

Section: Introductionmentioning

confidence: 99%

“…In principle, DINeC as desorption/ionization source can be coupled to any type of mass spectrometer. However, the combination with ion trap mass spectrometry features two main advantages: first, the pulse width and repetition rate of a typical pulsed cluster beam correspond very well to the discontinuous accumulation time as well as the spectral rate of the ion trap 15,32 . Second, the soft nature of the DINeC process leads to desorption of intact molecules.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Bomhardt

Schneider

Portz

et al. 2020

JoVE

Self Cite

View full text Add to dashboard Cite

Desorption/Ionization Induced by Neutral SO 2 Clusters (DINeC) is employed as a very soft and efficient desorption/ionization technique for mass spectrometry (MS) of complex molecules and their reactions on surfaces. DINeC is based on a beam of SO 2 clusters impacting on the sample surface at low cluster energy. During cluster-surface impact, some of the surface molecules are desorbed and ionized via dissolvation in the impacting cluster; as a result of this dissolvation-mediated desorption mechanism, low cluster energy is sufficient and the desorption process is extremely soft. Both surface adsorbates and molecules of which the surface is composed of can be analyzed. Clear and fragmentationfree spectra from complex molecules such as peptides and proteins are obtained. DINeC does not require any special sample preparation, in particular no matrix has to be applied. The method yields quantitative information on the composition of the samples; molecules at a surface coverage as low as 0.1 % of a monolayer can be detected. Surface reactions such as H/D exchange or thermal decomposition can be observed in real-time and the kinetics of the reactions can be deduced. Using a pulsed nozzle for cluster beam generation, DINeC can be efficiently combined with ion trap mass spectrometry. The matrix-free and soft nature of the DINeC process in combination with the MS n capabilities of the ion trap allows for very detailed and unambiguous analysis of the chemical composition of complex organic samples and organic adsorbates on surfaces. Video Link The video component of this article can be found at https://www.jove.com/video/60487/ 11,12,13,14,15,16,17. DINeC is based on a beam of molecular clusters which consist of 10 3 to 10 4 SO 2 molecules (Figure 1). When the clusters impact on the sample, they interact in various ways with the molecules on and in the surface: first, a part of the cluster's kinetic energy is redistributed and activates desorption. Similarly

show abstract

“…Thanks to the development of huge cluster beams such as C 60 ions, Ar cluster ions, H 2 O cluster ions and SO 2 neutral cluster beams, softer ionization, which enables easier high mass ion detection, became feasible in time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) measurement . TOF‐SIMS has been applied to the analysis of a variety of materials including biomaterials because it has an extremely high sensitivity for detecting one molecular layer (less than 2 nm) and high spatial resolution for chemical mapping.…”

mentioning

confidence: 99%

Peptide fragmentation caused by Ar cluster ions depending on primary ion energy

Aoyagi

Kawashima

Yokoyama

2015

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Energy-dependence fragmentation helps in understanding the peptide spectra. Specific peptide fragment ions of the larger peptides are likely to be detected under lower energy than energy higher than 10 eV/atom. Although it is difficult to interpret the TOF-SIMS spectra of a peptide obtained with an Ar cluster ion beam, the secondary ions can be classified by comparing those obtained with different energy Ar cluster ion beams.

show abstract

Desorption/ionization induced by neutral cluster impact as a soft and efficient ionization source for ion trap mass spectrometry of biomolecules

Cited by 25 publications

References 28 publications

Electron microscopy of polyoxometalate ions on graphene by electrospray ion beam deposition

Electron microscopy of polyoxometalate ions on graphene by electrospray ion beam deposition

Analysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry

Peptide fragmentation caused by Ar cluster ions depending on primary ion energy

Contact Info

Product

Resources

About