Destructive stationary phase gradients for reversed-phase/hydrophilic interaction liquid chromatography

In situ silanization for continuous stationary phase gradients on particle packed LC columns

“…Simulation 14 and experimental 11,13 data have demonstrated that continuous stationary phase gradients can offer novel selectivity and the potential for peak focusing. However, the fabrication of such gradients on particle packed columns is a major challenge and thus a signicant obstacle to furthering research on continuous stationary phase gradient columns.…”

“…To our knowledge, only one report has appeared on a continuous stationary phase gradient on a particle packed column. 13 In that work, the stationary phase gradient was created on a commercially modied C 18 column via the timedependent acid hydrolysis and hydrolytic cleavage of C 18 ligands. 13 For columns that have already been modied with an organic constituent, this is a viable approach for creating a stationary phase gradient on a packed column.…”

“…13 In that work, the stationary phase gradient was created on a commercially modied C 18 column via the timedependent acid hydrolysis and hydrolytic cleavage of C 18 ligands. 13 For columns that have already been modied with an organic constituent, this is a viable approach for creating a stationary phase gradient on a packed column. However, there is still a need for the development of an in situ approach for the creation of a chemical gradient on a bare silica packed columns.…”

“…The interest in stationary phase gradients stems from a chromatographer's desire to improve selectivity and resolution, and provide possible peak focusing when a stationary phase gradient is coupled with a mobile phase gradient. 13,14 In contrast to discontinuous gradients, continuous gradients have the added benet of novel selectivities that could arise from neighboring ligand effects, which can occur when two ligands are adjacent on a single chromatographic support. 11,15 Compared to traditional mixed-mode chromatography, which has a xed ratio between the moieties along the length of the column, 16 the ligand ratio on a stationary phase gradient is not constant.…”

Pembuatan Kolom Monolit Mixed-Mode untuk Pemisahan Fenol dalam Kromatografi Cair Sistem Kapiler

“…Mixed-Mode Liquid Chromatography (MMLC) merupakan suatu teknik pemisahan kromatografi cair untuk memisahkan kombinasi senyawa yang bersifat hidrofilik dan hidrofobik yang sulit dilakukan hanya dengan menggunakan satu kolom pemisah pada HPLC metode tunggal. MMLC telah banyak berkembang untuk mengatasi permasalah dalam pemisahan sampel protein, alkilbenzen, amina dan nukleosida [9][10][11][12].…”