Desulfovibrio fairfieldensis-Derived Outer Membrane Vesicles Damage Epithelial Barrier and Induce Inflammation and Pyroptosis in Macrophages

Nie, Yawen; Xie, Xiao-Qian; Zhou, Lingxi; Guan, Qijie; Ren, Yilin; Mao, Yong; Shi, Jin‐Song; Xu, Zhenghong; Geng, Yan

doi:10.3390/cells12010089

Cited by 20 publications

(13 citation statements)

References 41 publications

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“…Desulfovibrio_fairfieldensis is an opportunistic pathogen that is widespread in the human gut and can cause serious infectious diseases. 59 Interestingly, no detectable changes in Lactobacillus_taiwanensis were seen in any of the four immunized groups at 16 weeks compared to 2 weeks post-immunization, while two probiotics, Lactobacillus_reuteri and Lactobacillus_ intestinalis, increased significantly during that time. Meanwhile, the unclassified_Lachnospiraceae_NK4A136_ group bacteria in the rEg.P29 T + CpG group were significantly elevated, which was highly consistent with the results of previous studies.…”

Section: Discussionmentioning

confidence: 83%

“…This may be attributed to the fact that vaccine immunization triggers a strong immune response in mice, leading to a decline in some of the flora that have been implicated in the inflammatory response. Desulfovibrio_fairfieldensis is an opportunistic pathogen that is widespread in the human gut and can cause serious infectious diseases 59 . Interestingly, no detectable changes in Lactobacillus_taiwanensis were seen in any of the four immunized groups at 16 weeks compared to 2 weeks post‐immunization, while two probiotics, Lactobacillus_reuteri and Lactobacillus_intestinalis, increased significantly during that time.…”

Section: Discussionmentioning

confidence: 96%

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Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Zhang,

Lv,

Zhao

et al. 2023

Immunity Inflam & Disease

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ObjectiveCystic echinococcosis (CE), a zoonotic parasitic disease caused by Echinococcus granulosus, remains a public health and socioeconomic issue worldwide, making its prevention and treatment of vital importance. The aim of this study was to investigate changes in the intestinal microbiota of mice immunized with three peptide vaccines based on the recombinant antigen of E. granulosus, P29 (rEg.P29), with the hope of providing more valuable information for the development of vaccines against CE.MethodsThree peptide vaccines, rEg.P29T, rEg.P29B, and rEg.P29T + B, were prepared based on rEg.P29, and a subcutaneous immunization model was established. The intestinal floras of mice in the different immunization groups were analyzed by 16 S rRNA gene sequencing.ResultsThe intestinal microbiota analysis at both immunization time points revealed that Firmicutes, Bacteroidota, and Verrucomicrobiota were the predominant flora at the phylum level, while at the genus level, Akkermansia, unclassified_Muribaculaceae, Lachnospiraceae_NK4A136_group, and uncultured_rumen bacterium were the dominant genera. Some probiotics in the intestines of mice were significantly increased after immunization with the peptide vaccines, such as Lactobacillus_taiwanensis, Lactobacillus_reuteri, Lachnospiraceae_NK4A136_group, Bacteroides_acidifaciens, and so forth. Meanwhile, some harmful or conditionally pathogenic bacteria were decreased, such as Turicibacter sanguinis, Desulfovibrio_fairfieldensis, Clostridium_sp, and so forth, most of which are associated with inflammatory or infectious diseases. Kyoto Encyclopaedia of Genes and Genomes enrichment analysis revealed that the differential flora were enriched in multiple metabolic pathways, primarily biological systems, human diseases, metabolism, cellular processes, and environmental information processing.ConclusionIn this study, we comprehensively analyzed and compared changes in the intestinal microbiota of mice immunized with three peptide vaccines as well as their related metabolic pathways, providing a theoretical background for the development of novel vaccines against E. granulosus.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 96%

Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Zhang,

Lv,

Zhao

et al. 2023

Immunity Inflam & Disease

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“…Our study also showed that DSV may promote the growth of D. fairfieldensis , which may be an invasive and pathogenic genus and can cause severe bacteremia 72 . D. fairfieldensis -derived outer membrane vesicles are found to damage the intestinal epithelial barrier and activate intrinsic inflammation 73 . The relationship between D. desulfuricans with other Desulfovibrio species are also worth investigating in the future to evaluate their different impacts on liver health.…”

Section: Discussionmentioning

confidence: 99%

Restricted intake of sulfur-containing amino acids reversed the hepatic injury induced by excessDesulfovibriothrough gut-liver axis

Zhou,

Lu,

Nie

et al. 2024

Preprint

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Gut-liver axis has been a study focus for liver diseases. Diet is a key player in influencing the gut microbiota. However, the effect of different dietary patterns on gut microbiota and liver functions remains unclear. Here, we used mouse standard chow and purified diet to mimic two common human dietary patterns: healthy grain and planted-based diet and Western style diet, respectively and explored their impacts on the gut microbiota and liver. Gut microbiota experienced a great shift with notable increase inDesulfovibrio, gut bile acid (BA) concentration elevated significantly, and liver inflammation was observed in mice fed with the purified diet. Liver inflammation due to translocation of toxic lipopolysaccharides (LPS) and hydrophobic BAs from the damaged gut barrier was also observed in mice fed with the chow diet after receivingDesulfovibrio desulfuricansATCC 29577 (DSV). Restricted intake of sulfur-containing amino acids reversed the liver injury due to excessDesulfovibriothrough lowering the gut BA concentration and enhancing the hepatic antioxidant and detoxifying ability.Ex vivofermentation of human fecal microbiota with primary BAs also demonstrated that DSV enhanced production of secondary BAs. Germ-free mice had higher concentration of both conjugated and unconjugated primary BAs in their gut after receiving DSV.

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“…It may contribute to regulating the cervical microenvironment and facilitating normal cervical maturation. Desulfovibrio fairfieldensis [ 49 ] is associated with inflammatory bowel disease and may impact cervical maturation progression through inflammation. Surprisingly, its abundance is lower in the immature cervix group, necessitating further investigation into the underlying reasons.…”

Section: Discussionmentioning

confidence: 99%

Characteristics of cervicovaginal microflora at different cervical maturity during late pregnancy: A nested case-control study

Chen,

Hu,

Zheng

et al. 2024

PLoS ONE

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Objective The mechanism of cervical ripening in late pregnancy is still unclear. The vaginal microbiome has been reported to correlate with the preterm birth and short cervix in pregnant women. However, the associations between the cervical maturity and the vaginal microbiome are still poorly understood. We aim to analyze the cervicovaginal microflora in women with ripe cervix and in those who are unripe when delivering at term. Methods Cervicovaginal swabs were collected between 40 and 41 weeks of gestation from the following 2 different groups of patients: ripe group (n = 25) and unripe group (n = 25). Samples were tested using 16S ribosomal RNA gene high-throughput sequencing and analyzed by bioinformatics platform. Results This study highlights the relationship between cervical maturity during late pregnancy and the composition of the cervicovaginal microflora. Both α- and β-diversity analyses demonstrated significant differences between women with a ripe cervix and those with an unripe cervix. Notably, the Lactobacillus profile was found to be closely linked to cervical maturity. There was a significant difference in the vaginal community state type, with CST IV being more prevalent in women with an unripe cervix. Furthermore, the association between CST IV and the unripe cervix group, as indicated by the odds ratio of 8.6, underscores its relevance in evaluating cervical maturity, when compared to other Lactobacillus-dominant community state types. Additionally, several bacterial taxa, particularly Lactobacillus, exhibited differential relative abundances between the two groups. Conclusion This study provided significant evidence regarding the relationship between the vaginal microbiome and cervical maturity, highlighting the differential diversity, community state types, and specific bacterial taxa, such as Lactobacillus, that are associated with cervical maturation status. These findings contributed to our understanding of the dynamics of the cervicovaginal microflora during late pregnancy and its implications for cervical health.

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Desulfovibrio fairfieldensis-Derived Outer Membrane Vesicles Damage Epithelial Barrier and Induce Inflammation and Pyroptosis in Macrophages

Cited by 20 publications

References 41 publications

Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Restricted intake of sulfur-containing amino acids reversed the hepatic injury induced by excessDesulfovibriothrough gut-liver axis

Characteristics of cervicovaginal microflora at different cervical maturity during late pregnancy: A nested case-control study

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