Detecção de locos de características quantitativas nos cromossomos 1, 2, 3, 12, 14, 15 e X de suínos: características de desempenho

Paixão, Débora Martins; Neto, José Braccini; Paiva, Samuel Rezende; Carneiro, Paulo Luız Souza; Pinto, Ana Paula Gomes; Sousa, Katiene Régia Silva; Nascimento, Carlos Souza do; Verardo, Lucas Lima; Hidalgo, A.M.; Lopes, Paulo Sávio; Guimarães, Simone Eliza Facioni

doi:10.1590/s0102-09352013000100031

Cited by 2 publications

(4 citation statements)

References 23 publications

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“…Eight of these had not been reported in the consulted literature: SF and LUNG on SSC4; SBF, PBF and LRBF on SSC8; BW on SSC17; SIL and BCW on SSCX (PigQTLdb). Compared to previous studies that used the same F2 population and relied only on microsatellite markers ( Paixão et al , 2008 , 2012 , 2013 ; Silva et al , 2008 ; Sousa et al , 2011 ), only seven of 40 QTL were confirmed in the present analysis. For these confirmed QTL the confidence intervals were narrowed down on average by 23.9 cM using the dataset with increased marker coverage.…”

Section: Discussioncontrasting

confidence: 87%

“…On SSC1 four new QTL were detected from this resource family. The QTL affecting SA was considered a new QTL because the SA QTL detected by Paixão et al (2013) on this chromosome was located at a different position, in a different marker interval. We detected a QTL affecting BW near a QTL previously reported by Knott et al (1998) and Beeckmann et al (2003) , located in the proximal region of the chromosome.…”

Section: Discussionmentioning

confidence: 99%

“…These final 237 SNPs were distributed as follows: SSC1 (56), SSC4 (54), SSC7 (59), SSC8 (31), SSC17 (25), and SSCX (12). Genotypes from 37 microsatellite markers were available from previous studies ( Paixão et al , 2008 , 2012 , 2013 ; Silva et al , 2008 ; Sousa et al , 2011 ) and included in the analyses ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies from our research group were conducted on the same population and detected QTL by means of microsatellite markers. A combined total of 40 QTL for growth performance, meat quality, internal organs, cut yield, and carcass composition were found in studies by Paixão et al ( 2008 , 2012 , 2013 ), Silva et al (2008) , and Sousa et al (2011) : five on chromosome 1 (SSC1), 12 on chromosome 4 (SSC4), nine on chromosome 7 (SSC7), eight on chromosome 8 (SSC8), three on chromosome 17 (SSC17), and three on chromosome X (SSCX). The sparse genetic maps that were used led to the detection of QTL with large confidence intervals.…”

Section: Introductionmentioning

confidence: 96%

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Fine mapping and single nucleotide polymorphism effects estimation on pig chromosomes 1, 4, 7, 8, 17 and X

et al. 2013

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Fine mapping of quantitative trait loci (QTL) from previous linkage studies was performed on pig chromosomes 1, 4, 7, 8, 17, and X which were known to harbor QTL. Traits were divided into: growth performance, carcass, internal organs, cut yields, and meat quality. Fifty families were used of a F2 population produced by crossing local Brazilian Piau boars with commercial sows. The linkage map consisted of 237 SNP and 37 microsatellite markers covering 866 centimorgans. QTL were identified by regression interval mapping using GridQTL. Individual marker effects were estimated by Bayesian LASSO regression using R. In total, 32 QTL affecting the evaluated traits were detected along the chromosomes studied. Seven of the QTL were known from previous studies using our F2 population, and 25 novel QTL resulted from the increased marker coverage. Six of the seven QTL that were significant at the 5% genome-wide level had SNPs within their confidence interval whose effects were among the 5% largest effects. The combined use of microsatellites along with SNP markers increased the saturation of the genome map and led to smaller confidence intervals of the QTL. The results showed that the tested models yield similar improvements in QTL mapping accuracy.

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Section: Discussioncontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Fine mapping and single nucleotide polymorphism effects estimation on pig chromosomes 1, 4, 7, 8, 17 and X

et al. 2013

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Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace × Large White crossbred) pigs vaccinated against Pasteurella multocida type D

Sousa

Ribeiro

Dantas

et al. 2017

Research in Veterinary Science

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We aimed to compare Toll-like receptors (TLR) and cytokines expression in local Piau breed and a Commercial line (Landrace×Large White crossbred) pigs in response to vaccination against Pasteurella multocida type D. Seronegative gilts for Pasteurella multocida type D and Mycoplasma hyopneumoniae were used, from which peripheral blood mononuclear cells (PBMC) were collected in four time points (T0, T1, T2 and T3; before and after each vaccination dose). For bronchoalveolar lavage fluid cells (BALF), we set groups of vaccinated and unvaccinated animals for both genetic groups. Gene expression was evaluated on PBMC and BALF. In PBMC, when we analyzed time points within breeds, significant differences in expression for TLRs and cytokines, except TGFβ, were observed for Commercial animals. For the Piau pigs, only TGFβ showed differential expression. Comparing the expression among genetic groups, the Commercial pigs showed higher expression for TLRs after first vaccination dose, while for IL2, IL6, IL12 and IL13, higher expression was also observed in T3 and IL8 and IL10, in T1 and T3. Still comparing the breeds, the crossbred animals showed higher expression for TNFα in T1 and T2, while for TGFβ only in T2. For gene expression in BALF, vaccinated Commercial pigs showed higher expression of TLR6, TLR10, IL6, IL8, IL10, TNFα and TGFβ genes than vaccinated Piau pigs. The Commercial line pigs showed higher sensitivity to vaccination, while in local Piau breed lower responsiveness, which may partly explain genetic variability in immune response and will let us better understand the tolerance/susceptibility for pasteurellosis.

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Detecção de locos de características quantitativas nos cromossomos 1, 2, 3, 12, 14, 15 e X de suínos: características de desempenho

Cited by 2 publications

References 23 publications

Fine mapping and single nucleotide polymorphism effects estimation on pig chromosomes 1, 4, 7, 8, 17 and X

Fine mapping and single nucleotide polymorphism effects estimation on pig chromosomes 1, 4, 7, 8, 17 and X

Comparison of gene expression of Toll-like receptors and cytokines between Piau and Commercial line (Landrace × Large White crossbred) pigs vaccinated against Pasteurella multocida type D

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