Detecção rápida de Salmonella Enteritidis em alimentos por ensaio imunoenzimático ELISA

Alcocer, Iliana; Oliveira, Tereza Cristina Rocha Moreira de

doi:10.1590/s0101-20612003000300019

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Therefore, the development of rapid tests is essential for the diagnosis and the control of Salmonella spp. (Alcocer & Oliveira 2003). Microbiological diagnosis of pathogens to determine food safety has remained in use for many years, because it is an established and effective technique, although time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

et al. 2018

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Salmonella detection is a key point in food safety testing, because of the frequent association of this pathogen with food poisoning in humans. The standard bacteriological tests currently used for Salmonella-detection are time-consuming; therefore, there is a need to develop alternative methods to accelerate the detection. In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg), Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium). The efficiency of the technique, represented by the time required for detection of positive and negative samples, was compared with that of the standard diagnostic tests used for this pathogen, the bacteriological assay and the polymerase chain reaction (PCR)-based test. This study confirmed the ability of the bacteriophage-associated immunomagnetic separation assay to identify 99.6% of Salmonella-positive samples of the three serovars tested. In contrast, the bacteriological assay and PCR-based test detected 95.1% and 98.5% of the Salmonella-positive samples respectively.

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Section: Introductionmentioning

confidence: 99%

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

et al. 2018

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“…in foods have been evaluated, including immunoassays (Walker et al, 2001;Alcocer and Oliveira, 2003;von Ruckert et al, 2008) and hybridization of nucleic acids and PCR (Li et al, 2000;Cortez et al, 2006;Myint et al, 2006;von Ruckert et al, 2008). The main advantages of the PCR are the capacity to detect target sequences, regardless of the antigen expression of the target cells; a higher sensitivity; and shorter time demanded to process the samples, mainly when compared with the conventional methodologies of pathogen isolation (Bennett et al, 1998;Rychlik et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a polymerase chain reaction protocol for the detection of Salmonella species directly from superficial samples of chicken carcasses and preenrichment broth

Matias¹,

Pinto²,

Cossi³

et al. 2010

Poultry Science

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Chicken meat is an important vehicle of foodborne pathogens, such as Salmonella spp., and demands a systematic control of microbiological contamination during industrial processing. This control occurs by the adoption of quality control systems in slaughters based on the microbiological investigation on hygiene indicators and pathogens, requiring the development of fast, trustable, and precise methodologies. The objective of this study was to compare the Salmonella spp. conventional methodology to a protocol of PCR in chicken carcass surface samples. The PCR protocol was developed directly from the collected samples and from preenrichment broth before and after incubation. The obtained results were compared by chi(2) and McNemar tests (P < 0.05), and the values of concordance, sensitivity, and specificity of PCR variations were calculated considering the conventional methodology as a parameter. The obtained results indicated that although some similarities between the methodologies were observed when positive results were considered (P > 0.05), the PCR developed from preenrichment after incubation presented significant differences from all the other methodologies (P < 0.05). Wide variations were observed in the PCR performance for Salmonella spp. detection in chicken carcasses, which can be due to intrinsic factors inherent to the achievement of this food. Further studies are necessary to elucidate the applicability of the PCR as a tool for microbiological monitoring in quality control systems for chicken processing.

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Phenotypic and Genotypic Eligible Methods for Salmonella Typhimurium Source Tracking

2017

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Salmonellosis is one of the most common causes of foodborne infection and a leading cause of human gastroenteritis. Throughout the last decade, Salmonella enterica serotype Typhimurium (ST) has shown an increase report with the simultaneous emergence of multidrug-resistant isolates, as phage type DT104. Therefore, to successfully control this microorganism, it is important to attribute salmonellosis to the exact source. Studies of Salmonella source attribution have been performed to determine the main food/food-production animals involved, toward which, control efforts should be correctly directed. Hence, the election of a ST subtyping method depends on the particular problem that efforts must be directed, the resources and the data available. Generally, before choosing a molecular subtyping, phenotyping approaches such as serotyping, phage typing, and antimicrobial resistance profiling are implemented as a screening of an investigation, and the results are computed using frequency-matching models (i.e., Dutch, Hald and Asymmetric Island models). Actually, due to the advancement of molecular tools as PFGE, MLVA, MLST, CRISPR, and WGS more precise results have been obtained, but even with these technologies, there are still gaps to be elucidated. To address this issue, an important question needs to be answered: what are the currently suitable subtyping methods to source attribute ST. This review presents the most frequently applied subtyping methods used to characterize ST, analyses the major available microbial subtyping attribution models and ponders the use of conventional phenotyping methods, as well as, the most applied genotypic tools in the context of their potential applicability to investigates ST source tracking.

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Detecção rápida de Salmonella Enteritidis em alimentos por ensaio imunoenzimático ELISA

Cited by 3 publications

References 19 publications

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

Comparison of three diagnostic methods for Salmonella enterica serovars detection in chicken rinse

Evaluation of a polymerase chain reaction protocol for the detection of Salmonella species directly from superficial samples of chicken carcasses and preenrichment broth

Phenotypic and Genotypic Eligible Methods for Salmonella Typhimurium Source Tracking

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