1986
DOI: 10.1002/ajmg.1320250314
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Detecting abnormal human chromosome constitutions by dual laser flow cytogenetics

Abstract: Our custom dual laser chromosome sorter has been used to characterize and isolate metaphase human chromosomes rapidly for gene mapping purposes. Herein, we tested how well this system could detect unknown abnormal human chromosome constitutions. These results were compared to those of conventional cytogenetic analyses by banding and photomicrography. The sorter was used to analyze each cell line stained with two different stain pairs: DIPI-chromomycin and Hoechst-chromomycin. In 20 min, two histograms represen… Show more

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Cited by 25 publications
(7 citation statements)
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“…This provides an accurate measurement of the frequency of different chromosome types. For instance, trisomy 21 appears as a 50-% increase in the volume of peak representing chromosome 21 as compared with other chromosome types (Gray et al 1986), and translocations resulting in derivative chromosomes that differ either in DNA content or base pair ratio will appear as new peaks (Lebo et al 1986). Chromosome fluorescence can be measured with coefficients of variation as low as 1 %, and the size of small deletions can be estimated (Trask et al 1996).…”
Section: Chromosome Characterization By Flowmentioning
confidence: 99%
“…This provides an accurate measurement of the frequency of different chromosome types. For instance, trisomy 21 appears as a 50-% increase in the volume of peak representing chromosome 21 as compared with other chromosome types (Gray et al 1986), and translocations resulting in derivative chromosomes that differ either in DNA content or base pair ratio will appear as new peaks (Lebo et al 1986). Chromosome fluorescence can be measured with coefficients of variation as low as 1 %, and the size of small deletions can be estimated (Trask et al 1996).…”
Section: Chromosome Characterization By Flowmentioning
confidence: 99%
“…An extensive series of flow karyotype analyses of human cell lines with cytogenetic abnormalities by bi variate analysis demonstrated that most chromosome aberrations could be detected by flow cytometry [9]. Flow analysis can be used to detect an aneuploid number of normal chromosomes, most non-reciprocal transloca tions, most reciprocal translocations that exchange dif ferent length chromosome segments or equal size seg ments with different amounts of Hoechst and chromo mycin bands.…”
Section: Flow Karyotype Analysismentioning
confidence: 99%
“…In many cases, the origin of the translocated chromo somes can be identified by the loss of counts in the nor mal chromosome peaks from which they were derived [9].…”
Section: Flow Karyotype Analysismentioning
confidence: 99%
“…Bivariate flow karyotyping of human chromosomes has proven to be a helpful tool in the cytogenetic study of congenital and other diseases (1, 2, 5, 9, 14,16,17,24). It can be used to detect several types of aberrations: (1) numerical changes, (2) most nonreciprocal translocations, (3) most reciprocal translocations that exchange different length chromosome segments or equal size segments with different quantities of HO and CA bands, and (4) chromosomal deletions or inse tions.…”
mentioning
confidence: 99%
“…It can be used to detect several types of aberrations: (1) numerical changes, (2) most nonreciprocal translocations, (3) most reciprocal translocations that exchange different length chromosome segments or equal size segments with different quantities of HO and CA bands, and (4) chromosomal deletions or inse tions. The size of deletions can be readily estimate1 The accurate detection of deletions or insertions 1 about one-tenth of the total chromosome length up to megabasepairs has been reported (14,17,20,24).…”
mentioning
confidence: 99%