Detecting and exploring partially unfolded states of proteins using a sensor with chaperone bound to its surface

George, Doaa F.; Bilek, Marcela M.M.; McKenzie, David R.

doi:10.1016/j.bios.2008.07.076

Cited by 6 publications

(1 citation statement)

References 22 publications

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“…Sensing of environmental toxins is now possible with sensors based on biomolecular interactions (Frisk et al 2009). Detecting stressors that unfold target proteins which then bind to immobilized molecular chaperones has been proposed (George et al 2008). Enzymes are commonly used to catalyze chemical reactions in food and chemical processing.…”

Section: Introductionmentioning

confidence: 99%

Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

2010

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Plasma modification and plasma polymer deposition are valuable technologies for the preparation of surfaces for the covalent binding of biomolecules for applications such as biosensors, medical prostheses, and diagnostic devices as well as surfaces for enzyme-mediated reactions. Covalency is conveniently tested by the ability of the surface to retain the attached molecules after vigorous washing with sodium dodecyl sulphate (SDS). Covalency is indicated if the fraction of protein retained lies above the curve characteristic of physisorption. Confidence in covalency is strengthened when the washing protocol is aggressive enough to remove all adsorbed protein from a control significantly more hydrophobic than the test surface. The use of linker chemistry to space the molecules from the surface is in some cases beneficial. However, the use of linker chemistry is not necessary to retain molecular function for long periods when the polymer surface is modified by energetic bombardment. The energetic bombardment retains hydrophilicity of the surface by crosslinking the subsurface, and this appears to facilitate retention of protein function. Energetic bombardment also increases the functional life of molecules immobilized and then freeze dried on plasma-modified surfaces. Analysis of the surfaces shows that the covalent binding mechanism is related to the presence of free radicals on the surface and in the subsurface regions. The unpaired electrons associated with the radicals appear to be mobile within the modified region and can diffuse to the surface to take part in binding interactions. Proactive implantable devices can make use of these principles of covalent attachment by seeding the surface of an implant with a biomolecule that elicits the desired interaction with cells and prevents undesirable responses.

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Section: Introductionmentioning

confidence: 99%

Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

2010

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Grading the commercial optical biosensor literature—Class of 2008: ‘The Mighty Binders’

Rich

Myszka

2009

J of Molecular Recognition

143

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Optical biosensor technology continues to be the method of choice for label-free, real-time interaction analysis. But when it comes to improving the quality of the biosensor literature, education should be fundamental. Of the 1413 articles published in 2008, less than 30% would pass the requirements for high-school chemistry. To teach by example, we spotlight 10 papers that illustrate how to implement the technology properly. Then we grade every paper published in 2008 on a scale from A to F and outline what features make a biosensor article fabulous, middling or abysmal. To help improve the quality of published data, we focus on a few experimental, analysis and presentation mistakes that are alarmingly common. With the literature as a guide, we want to ensure that no user is left behind.

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Direct Evidence of Covalent Immobilisation of Microperoxidase‐11 on Plasma Polymer Surfaces

Yin

Bilek

McKenzie

2010

Plasma Processes & Polymers

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The question of whether immobilised proteins are bound covalently on plasma activated or deposited polymers has been a challenge for almost 30 years, as there has been no directly evidence to conclude unambiguously. In this paper, we report evidence that a chemical binding reaction occurred during immobilisation of microperoxidase‐11 (MP‐11) proteins on plasma polymer surfaces. Untreated polymer surfaces were not resistant to detergent cleaning and did not show any increase of S 2p component after protein immobilisation, whilst all plasma‐deposited surfaces showed large amounts of immobilised MP‐11 proteins after detergent cleaning. We conclude that the MP‐11 protein had a chemical binding reaction thorough its cysteine residues with the plasma polymer surfaces.

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Detecting and exploring partially unfolded states of proteins using a sensor with chaperone bound to its surface

Cited by 6 publications

References 22 publications

Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants

Grading the commercial optical biosensor literature—Class of 2008: ‘The Mighty Binders’

Direct Evidence of Covalent Immobilisation of Microperoxidase‐11 on Plasma Polymer Surfaces

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