Detecting cells on the surface of a silver electrode quartz crystal microbalance using plasma treatment and graft polymerization

Chou, Hung-Che; Yan, Tsong‐Rong; Chen, Ko-Shao

doi:10.1016/j.colsurfb.2009.05.026

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…Other studies relating to cell adhesion focused on the viscoelastic properties of the extracellular adhesives produced by marine diatoms Amphora coffeaeformis Cleve and Craspedostauros australis Cox (Molino et al , 2008). The effect of mucin surface‐shielding on activation of immobilized neutrophils (Sandberg et al , 2009b), and other forms of surface grafting (Croll et al , 2006; Chou et al , 2009) involved integral use of the QCM.…”

Section: Cellsmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

163

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Since the publication of the original review of piezoelectric acoustic sensors in this series there has been a consistent, gradual expansion in the number of published papers using 'quartz crystal microbalances' (QCM). Between 2001 and 2009, the number of QCM publications per annum has increased from 49 to 273, with a two-fold increase in papers per annum between 2004 and 2008. Within the field, comparing the time covered by the current to the previous review, there are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase) and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterization of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This paper highlights theoretical and practical aspects of the principles that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and membrane interfaces.

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Section: Cellsmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

163

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“…The immobilization process was conducted as previously described (Chou, Yan, & Chen, 2009; Lin et al, 2007). All grafting equipment were internally developed and fabricated by Prof Ko‐Shao Chen of the Institute of Materials Engineering, Tatung University, Taipei 104, Taiwan, ROC.…”

Section: Methodsmentioning

confidence: 99%

Ultrahigh packing density next generation microtube array membrane: A novel solution for absorption‐based extracorporeal endotoxin removal device

Chew

Huang

et al. 2020

J Biomed Mater Res

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Sepsis is a deadly disease that is widely attributed to endotoxin released by gram‐negative bacterial infections often plague emergency care facilities. Conventionally antibiotics and vasopressors are used to treat this disease. Recent treatment protocol shifted to a membrane to remove the offending endotoxin monomer. Despite this shift, membrane‐based devices are often extremely costly, hindering accessibility to this life saving medical device. In view of this challenges, we adopted the internally developed polysulfone (PSF) microtube array membrane alternating (MTAM‐A) for use in blood sepsis treatment. PSF MTAM‐A were with polymyxin B (PMB) molecules immobilized were assembled into an internally developed cartridge housing and subjected to endotoxin removal models with water and blood spiked with 100 EU/ml of endotoxin as the feed solution. Samples were derived at 15, 30, 60, and 120 min and endotoxin levels were determined with limulus amebocyte lysate assay and benchmarked against the commercially available Toraymyxin device. The PSF MTAM‐A with 2.3 times the surface area was successfully fabricated and with PMB molecules immobilized, and assembled into a hemoperfusion device. Dynamic endotoxin removal test revealed and overall endotoxin removal capacity of 90% and a superior endotoxin removal efficiency that was significantly higher than that of Toraymyxin (internally conducted and reported). The data suggested that PSF MTAM‐A PMB membranes could potentially be applied in future hemoperfusion devices which would be significantly more efficient, compact, and affordable; potentially making such a life‐saving medical device widely available to the general public.

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“…[22,90, Moreover, the viscoelastic properties of living adherent cells (predominantly determined by the cytoskeleton) have been studied under the influence of diverse chemical, biological and physical stimuli by QCM sensing as well. QCM studies of CBAs described in literature cover various research fields, such as toxicity testing of chemical compounds and environmental pollutants [216,[243][244][245][246][247][248][249][250][251] , drug discovery [4,19,22,206,216,222,225,234,242,243,248,[251][252][253][254][255] , cytocompatibility testing of growth substrate materials and coatings [11,22,90,[216][217][218]221,224,228,230,232,234,236,[256][257][258][259] , cell receptor and signaling investigation…”

Section: Piezolectric Transducers For Label-free Cbb Qcmmentioning

confidence: 99%

Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors

Oberleitner

2018

Springer Theses

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These cellular responses and the accompanying changes of physicochemical parameters are what is to be detected and transferred into a measurable electrical signal by the transducer units of the label-free biosensor devices (Fig. 1-1). There are various types of transducers, distinguishable by the underlying physicochemical phenomenon: electrochemical (including potentiometric, amperometric, and impedimetric), optical, thermometric, piezoelectric, and magnetic transduction. [8] Tab. 1-1 gives an LAPSLight-addressable potentiometric sensors (LAPSs) are multilayer field-effect devices similar to ISFETs [66] . A LAPS consists of a doped semiconductor covered with an insulator layer and a chemosensitive layer. The device is immersed in an electrolyte solution. The semiconductor is connected with a reference electrode in contact with the electrolyte (Fig. 1-2 B; reviewed in [10,17,[67][68][69][70] with respect to biological and cell-based applications). The insulator material of LAPSs is typically made up either of a single SiO 2 layer 1.1 Label-free Biosensors for Cell-based Assays 7 chip-based systems for the label-free and continuous monitoring of DO in cell-based assays, i.e. the oxygen consumption rate (OCR) of cells. [47,48,50,53,54,57,[60][61][62] The sensor chips with integrated amperometric oxygen sensors are typically build up as multi-parametric sensors and contain further physicochemical transducer structures like e.g. for cell morphology testing and ECAR monitoring. Besides the direct measurement of DO, the amperometric sensors are also capable of sensing indirectly any species that is consumed or produced in an oxidase-based enzymatic reaction (amperometric enzyme sensor) via the oxygen or hydrogen peroxide concentration. For this purpose, the amperometric pO 2 sensor is coated with a layer in which the respective enzyme is immobilized and which enables free diffusion of all reactants between the bulk solution and the sensor surface. This approach was first presented by Clark and Lyons as an amperometric sensor for glucose [85] and has also been applied as transduction method in cell-based assays for the label-free determination and monitoring of glucose and lactose [78,79] . A list of physiologically relevant analytes that have been successfully monitored by amperometric enzyme sensors is given in [86] . ECISElectrical impedance-based sensing of adherent cells, also termed as electric cell-substrate impedance sensing (ECIS), utilizes the property of cells to impede AC current flow when they are in close contact to the substrate, i.e. a small gold-film working electrode (Fig. 1-2 D). This technique was presented first by Giaever and Keese in 1984 [87] as "morphological biosensor for mammalian cells" [88] . The principle of ECIS, the method of impedance spectroscopy, and the analysis, physiological meaning, and modeling of impedance data are extensively addressed in the methods section 3.4 Electric Cell-Substrate Impedance Sensing (ECIS). Briefly summarized, ECIS enables the time-resolved, label-free, and ...

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Detecting cells on the surface of a silver electrode quartz crystal microbalance using plasma treatment and graft polymerization

Cited by 9 publications

References 19 publications

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Ultrahigh packing density next generation microtube array membrane: A novel solution for absorption‐based extracorporeal endotoxin removal device

Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors

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