Detecting cow milk in sheep yoghurt by TaqMan real‐time PCR

Abstract

The detection of adulteration in the production of milk and other dairy products is crucial for economic reasons and consumer protection. In this study, yoghurts were produced from mixed milk (cow and sheep) in different ratios, and the percentage of sheep and cow milk was determined using TaqMan real-time polymerase chain reaction (PCR). 1% of cow milk was determined in the yoghurts and 0.01 ng of cow and sheep DNA was determined in sensitivity analyses. Therefore, TaqMan real-time PCR can be considered as a sensitive and reliable method for detecting cow DNA in yoghurt produced from mixed milk.

Сравнительная Оценка Спектральных Люминесцентных Характеристик Молока И Молочных Продуктов

“…The results obtained can be applied to create express control methods for processing and storing milk and dairy products. (Biçer & Sönmez, 2022) и другие методы Ashoorirad et al, 2021;Мельденберг и др., 2020). Хотя эти общепринятые методы точны и надежны, они разрушительны, отнимают значи тельное время, не всегда дешевы и экологически безопасны и могут использоваться только в лабо раторных условиях.…”