Detecting DNA Folding with Nanocapillaries

Steinbock, Lorenz J.; Otto, Oliver; Chimerel, Catalin; Gornall, J. L.; Keyser, Ulrich F.

doi:10.1021/nl100997s

Cited by 192 publications

(269 citation statements)

References 26 publications

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“…14,15,18,19 Under these conditions, the diffuse double layer is expected to be compressed to a sub-nanometer scale, and therefore undetectable, level according to: 50…”

Section: Characterization Of Nanopipettes In High Ionic Strength Mediamentioning

confidence: 99%

Characterization of Nanopipettes

et al. 2016

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Nanopipettes are widely used in electrochemical and analytical techniques as tools for sizing, sequencing, sensing, delivery and imaging. For all of these applications, the response of a nanopipette is strongly affected by its geometry and surface chemistry. As the size of nanopipettes becomes smaller, precise geometric characterization is increasingly important, especially if nanopipette probes are to be used for quantitative studies and analysis. This contribution highlights the combination of data from voltage-scanning ion conductivity experiments, transmission electron microscopy (TEM) and finite element method (FEM) simulations to fully characterize nanopipette geometry and surface charge characteristics, with an accuracy not achievable using existing approaches. Indeed, it is shown that presently used methods for nanopipette characterization can lead to highly erroneous information on nanopipettes. The new approach to characterization further facilitates high-level quantification of the behavior of nanopipettes in electrochemical systems, as demonstrated herein for a scanning ion conductance microscope (SICM) setup.

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“…14,15,18,19 Under these conditions, the diffuse double layer is expected to be compressed to a sub-nanometer scale, and therefore undetectable, level according to: 50…”

Section: Characterization Of Nanopipettes In High Ionic Strength Mediamentioning

confidence: 99%

Characterization of Nanopipettes

et al. 2016

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“…5 Besides solid-state nanopores in silicon dioxide, silicon nitride, or metal membranes, glass nanocapillaries have emerged in recent years as a cost-effective and versatile source of nanopores for single molecule detection. 6 They are fabricated using a laser pipettete puller, which heats the cylindrical hollow capillary and stretches it at the same time. This causes the glass capillary to shrink in diameter at the heated spot and finally break into two conical tips defined as nanocapillaries.…”

Section: T He Resistive Pulse Technique (Also Called Coulter Countermentioning

confidence: 99%

“…This allowed determining the diameter of every nanocapillary before assembling it into the measuring cell, which was not possible before. 6 SEM imaging was performed under a working distance between 2 and 9 mm, magnifications between 100k and 250k, beam currents between 10 and 800 pA, and acceleration voltages of 1−9 kV. EDX measurements were also possible, permitting it to measure the chemical composition before and after the shrinking.…”

Section: T He Resistive Pulse Technique (Also Called Coulter Countermentioning

confidence: 99%

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Steinbock

Radenović

2013

Nano Lett.

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The ability to reshape nanopores and observe their shrinkage under an electron microscope is a powerful and novel technique. It increases the sensitivity of the resistive pulse sensing and enables to detect very short and small molecules. However, this has not yet been shown for glass nanocapillaries. In contrast to their solid-state nanopore counterparts, nanocapillaries are cheap, easily fabricated and in the production do not necessitate clean room facilities. We show for the first time that quartz nanocapillaries can be shrunken under a scanning electron microscope beam. Since the shrinking is caused by the thermal heating of the electrons, increasing the beam current increases the shrink rate. Higher acceleration voltage on the contrary increases the electron penetration depth and reduces the electron density causing slower shrinkage. This allows us to fine control the shrink rate and to stop the shrinking process at any desired diameter. We show that a shrunken nanocapillary detects DNA translocation with six times higher signal amplitudes than an unmodified nanocapillary. This will open a new path to detect small and short molecules such as proteins or RNA with nanocapillaries.

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“…For silicon nitride membranes, the thickness was reduced recently to a few nanometres [25], while with graphene nanopores, the nanopore length could be cut down to a single atomic layer [16][17][18]. Recently, glass nanocapillaries were shown to be a relatively simple alternative approach for DNA sensing [26,27].…”

Section: Nanoporesmentioning

confidence: 99%

Controlling molecular transport through nanopores

Keyser

2011

J. R. Soc. Interface.

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173

191

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Nanopores are emerging as powerful tools for the detection and identification of macromolecules in aqueous solution. In this review, we discuss the recent development of active and passive controls over molecular transport through nanopores with emphasis on biosensing applications. We give an overview of the solutions developed to enhance the sensitivity and specificity of the resistive-pulse technique based on biological and solid-state nanopores.

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Detecting DNA Folding with Nanocapillaries

Cited by 192 publications

References 26 publications

Characterization of Nanopipettes

Characterization of Nanopipettes

Controllable Shrinking and Shaping of Glass Nanocapillaries under Electron Irradiation

Controlling molecular transport through nanopores

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