Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sample

Choi, Da-Yeon; Park, Ji-Na; Paek, Sanghyun; Choi, Seung-Cheol; Paek, Se‐Hwan

doi:10.1016/j.bios.2021.113828

Cited by 15 publications

(11 citation statements)

References 21 publications

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“…[37][38][39] Interestingly, the characteristics of exosomes vary depending on their origin, so they can also be utilized in biopsy-based diagnosis as biomarkers. [40][41][42][43][44][45] Moreover, several researchers have modied the surface or nucleic acid content of exosomes through cell engineering to expand their applications in nanobiotechnology, especially in the eld of DDSs. [46][47][48][49][50] Based on the valuable efforts of researchers, various types of exosomal DDSs have been developed and produced by several strategies using both physical and chemical processes and approaches.…”

Section: Introductionmentioning

confidence: 99%

“…37–39 Interestingly, the characteristics of exosomes vary depending on their origin, so they can also be utilized in biopsy-based diagnosis as biomarkers. 40–45 Moreover, several researchers have modified the surface or nucleic acid content of exosomes through cell engineering to expand their applications in nanobiotechnology, especially in the field of DDSs. 46–50…”

Section: Introductionmentioning

confidence: 99%

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Exosome-based drug delivery systems and their therapeutic applications

Lee

Chakraborty

et al. 2022

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exosome-based drug delivery systems and their therapeutic applications

Lee

Chakraborty

et al. 2022

RSC Adv.

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“…To solve the issues of centrifugal method, some new separation methods have been developed, such as magnetic bead system based on immunological binding and magnetic separation. Notably, in order to elute the captured exosomes from magnetic beads, non-physiological conditions of extreme ion concentrations are commonly involved, which may cause irreversible damage to exosomes during isolation and subsequently result in false information ( Gao et al, 2019 ; Choi et al, 2022 ). Moreover, exosome detection methods based on magnetic separation have obvious steric hindrance due to large particle sizes of magnetic beads and exosomes.…”

Section: Introductionmentioning

confidence: 99%

A light-activated magnetic bead strategy utilized in spatio-temporal controllable exosomes isolation

Wang

Zhang

Yang

et al. 2022

Front. Bioeng. Biotechnol.

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Tumor-derived exosomes are considered as a key biomarker in the field of liquid biopsy. However, conventional separation techniques such as ultracentrifugation, co-precipitation and column chromatography cannot isolate samples with high throughput, while traditional immunomagnetic separation techniques, due to steric effect of magnetic beads, reducing sensitivity of exosomes optical detection. Herein, we provide a novel and simple nanoplatform for spatiotemporally controlling extraction and elution of exosomes via magnetic separation and light-activated cargo release. In this system, magnetic beads are co-modified by photoresponsive groups -nitrobenzyl group and aptamers that are compatible with CD63-a highly expressed exosomal surface-specific protein. Through exosomes extracted from cell model and nude mice xenograft tumor model morphological characterization and proteomic analysis, results showed that our novel magnetic bead system outperformed current ultracentrifugation in serum exosome extraction in terms of extraction time, yield, and proportion of populations with high CD63 expression. This strategy may be a powerful tool for exosome isolation in clinical liquid biopsies of cancer disease.

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“…However, UC is time‐consuming and co‐isolates contaminants such as protein aggregates (Boriachek et al., 2018 ). Therefore, alternative methods such as asymmetric flow field‐flow fractionation (Zhang & Lyden, 2019 ), capillary‐channelled polymer fibre‐based chromatographic method (Jackson et al., 2021 ), exosome detection via the ultrafast‐isolation system: EXODUS (Chen et al., 2021 ), size exclusion chromatography (SEC) (Oeyen et al., 2018 ), ultrafiltration (Woo et al., 2017 ), polymeric precipitation (Doyle & Wang, 2019 ), and immunoaffinity (Choi et al., 2022 ; Zhu et al., 2021 ), have also been proposed to isolate EVs for varied downstream applications with different merits and limitations, which summarized in Table S1 . Nevertheless, it remains a challenge to isolate plasma EVs with preferable yield in a clinical‐friendly manner.…”

Section: Introductionmentioning

confidence: 99%

Rapid and efficient isolation platform for plasma extracellular vesicles: EV‐FISHER

Pan

Feng

Luo

et al. 2022

J of Extracellular Vesicle

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Extracellular vesicles (EVs) have found diverse applications in clinical theranostics. However, the current techniques to isolate plasma EVs suffer from burdensome procedures and limited yield. Herein, we report a rapid and efficient EV isolation platform, namely, EV-FISHER, constructed from the metal-organic framework featuring cleavable lipid probes (PO 4 3− -spacer-DNA-cholesterol, PSDC). The EV-FISHER baits EVs from plasma by cholesterol and separates them with an ordinary centrifuge. The captured EVs could be released and collected upon subsequent cleavage of PSDC by deoxyribonuclease I. We conclude that EV-FISHER dramatically outperforms the ultracentrifugation (UC) in terms of time (∼40 min vs. 240 min), isolation efficiency (74.2% vs. 18.1%), and isolation requirement (12,800 g vs. 135,000 g). In addition to the stable performance in plasma, EV-FISHER also exhibited excellent compatibility with downstream single-EV flow cytometry, enabling the identification of glypican-1 (GPC-1) EVs for early diagnosis, clinical stages differentiation, and therapeutic efficacy evaluation in breast cancer cohorts. This work portrays an efficient strategy to isolate EVs from complicated biological fluids with promising potential to facilitate EVs-based theranostics.

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Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sample

Cited by 15 publications

References 21 publications

Exosome-based drug delivery systems and their therapeutic applications

Exosome-based drug delivery systems and their therapeutic applications

A light-activated magnetic bead strategy utilized in spatio-temporal controllable exosomes isolation

Rapid and efficient isolation platform for plasma extracellular vesicles: EV‐FISHER

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