Detection, Accumulation, Distribution, and Depletion of Furaltadone and Nifursol Residues in Poultry Muscle, Liver, and Gizzard

Barbosa, Jorge; Freitas, Andreia; Moura, Sara; Mourão, José Luís; Silveira, Maria Irene Noronha da; Ramos, Fernando

doi:10.1021/jf2029384

Cited by 40 publications

(28 citation statements)

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“…A c c e p t e d M a n u s c r i p t 8 The ENRO has a wide spectrum of activity and being widely used as veterinary drug to improve health conditions. It has good absorption and high bioavailability with good penetration into body tissues and found at high concentrations in the excretory organs, especially the liver with high withdrawal time.…”

Section: Discussionmentioning

confidence: 99%

“…[2] The indiscriminate use of ENRO without observing their withdrawal period may create hazards for human health throughout the country. This study aims to determine the residues of ENRO at marketing age by using HPLC with UV detection in broilers and to compare it with the maximum residual limit (MRL) recommended by FAO/WHO [7] and Barbosa [8] as 100 and 200 µg/kg for meat and liver respectively, to provide a considerable data on the ENRO residues regarding the human safety.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Enrofloxacin Residues in Commercial Broilers Using High Performance Liquid Chromatography

Aslam

Kousar

Javed

et al. 2016

International Journal of Food Properties

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The broad-spectrum antibacterial drugs are widely used in food producing animals. Suspected residues of these drugs in meat may have ill effects upon human health. The aim of the present study was to determine the enrofloxacin (ENRO) residues in broiler's meat and liver samples.Detection of ENRO residues in meat (n=75) and liver (n=75) samples was performed by high performance liquid chromatography with UV detector set at 268 nm using C 18 column. Overall mean residual concentrations of ENRO in meat and liver samples were 208±55 and 527±84 µg/kg respectively. This study revealed that 52% (39) meat and 78.7% (59) liver samples were positive for ENRO, out of these 58.3% (21) meat and 71.2% (42) liver samples were having residual concentration above the maximum residual limits. So it can be concluded that the usage Downloaded by [New York University] at 23:13 30 May 2015A c c e p t e d M a n u s c r i p t 2 of this contaminated meat may cause resistance in consumers and seems to be a public health threat.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Enrofloxacin Residues in Commercial Broilers Using High Performance Liquid Chromatography

Aslam

Kousar

Javed

et al. 2016

International Journal of Food Properties

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“…Nitrofuran antibiotics including furazolidone, furaltadone, nitrofurazone and nitrofurantoin are a class of synthetic broad spectrum antibiotics which have been widely used both prophylactically and therapeutically for various bacterial and protozoan infections in fish, prawn, bees, swine, cattle and poultry [1]. However, since the early 1990s, various experimental animal studies have shown that nitrofurans and their metabolites have potential mutagenicity and genotoxicity [2].…”

Section: Introductionmentioning

confidence: 99%

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Shen

Sun

et al. 2013

Talanta

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Publisher rights This is the author's version of a work that was accepted for publication in Talanta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Talanta, VOL 103, 01/2012 General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Cite this article as: Zhen-Lin Xu, Yu-Dong Shen, Yuan-Ming Sun, Katrina Campbell, Yuan-Xin Tian, Shi-Wei Zhang, Hong-Tao Lei and Yue-Ming Jiang, Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), Talanta, http://dx.doi.org/10. 1016/j.talanta.2012.10.059 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractA heterologous competitive indirect enzyme-linked immunosorbent assay (ciELISA) for the determination of the furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) was developed. AMOZ was derivatised with 2-(4-formylphenoxy)acetic acid or 2-(3-formylphenoxy)acetic acid to obtain two novel immunizing haptens. The ability of these haptens in producing specific polyclonal antibodies against the nitrophenyl derivative of AMOZ (NPAMOZ) was compared with that of traditional immunizing haptens (derivatised AMOZ with 3-carboxybenzaldehyle or 4-carboxybenzaldehyle). The results indicated that the novel immunizing haptens were able to produce antibodies with almost a two-fold improvement in sensitivity of the ciELISA for NPAMOZ in comparison with the existing antibody based ELISAs. The differences in sensitivity were explained by the molecular modeling of the lowest energy conformations of NPAMOZ and the haptens. Another novel hapten, derivatised AMOZ with 2-oxoacetic acid, was synthesized and used as a heterologous coating hapten. The results showed that this strategy of using only a partial structure of the target molecule as the coating hapten was able to obtain a two to three-fold improvement in sensitivity. This study provided a modern approach for the development of an immunoassay with improved sensitivity for the metabolites of nitrofuran antibiotics.

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“…For nitrofurazone, the situation was somewhat different, showing SEM levels in liver and muscle of up to 35 and 28 µg/kg, respectively, which on a molar basis were also much higher than observed for the other drugs. Barbosa et al (2011) fed 19-week-old laying hens with feed containing either 150 mg/kg of furaltadone or 100 mg/kg of nifursol for a period of 5 weeks followed by 3 weeks on clean feed. Animals were slaughtered at the end of the treatment or after the additional 3 weeks on clean feed, and muscle, liver and gizzards were examined for the parent compound and the side-chains, the latter without prior extraction of tissues.…”

Section: Transfer To Eggsmentioning

confidence: 99%

Scientific Opinion on nitrofurans and their metabolites in food

Panel¹

2015

EFS2

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Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human health related to the presence of nitrofurans in food and whether a reference point for action (RPA) of 1.0 µg/kg for the marker metabolites is adequate to protect public health. Data on occurrence of nitrofuran marker metabolites in food were extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF). The CONTAM Panel concluded that these data were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which a single nitrofuran marker metabolite is present at 1.0 µg/kg in foods of animal origin, excluding milk and dairy products. The mean chronic dietary exposure for this worst-case scenario would range from 3.3 to 8.0 and 1.9 to 4.3 ng/kg b.w. per day for toddlers and adults, respectively. Nitrofurans and their marker metabolites, generally, are genotoxic and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 10 5 or greater for carcinogenicity and at 2.5 × 10 3 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 10 4 calculated for nonneoplastic effects do not indicate a health concern.© European Food Safety Authority, 2015 However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances that are high potency carcinogens, such as nitrofurans. As nitrofurans are excluded from that opinion, and taking into account that the presence of SEM in food may be from sources other than use of nitrofurazone, the European Commission (EC) requested the European Food Safety Authority (EFSA) for a scientific opinion on the risks to human health related to the presence of nitrofurans and their metabolites in food. The opinion should include (a) an evaluation of the toxicity of nitrofurans and their metabolites for humans, considering all relevant toxicological endpoints and identification of the toxicological relevance of nitrofurans and their metabolites present in food, and (b) an exposure assessment of the EU population to nitrofurans and their metabolites from food, including the consumption patterns of specific (vulnerable) groups of the population. In addition, the opinion should assess the appropriateness of...

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Detection, Accumulation, Distribution, and Depletion of Furaltadone and Nifursol Residues in Poultry Muscle, Liver, and Gizzard

Cited by 40 publications

References 24 publications

Determination of Enrofloxacin Residues in Commercial Broilers Using High Performance Liquid Chromatography

Determination of Enrofloxacin Residues in Commercial Broilers Using High Performance Liquid Chromatography

Novel hapten synthesis for antibody production and development of an enzyme-linked immunosorbent assay for determination of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ)

Scientific Opinion on nitrofurans and their metabolites in food

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