Detection and Drug Susceptibility Testing of Neisseria gonorrhoeae Using Isothermal Microcalorimetry

Grütter, Anabel E.; Lafranca, Tecla; Sigg, Aurelia Pahnita; Mariotti, Max; Bonkat, Gernot; Braissant, Olivier

doi:10.3390/microorganisms9112337

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…Microcalorimetry has been used to determine antimicrobial susceptibility in different organisms and the results obtained so far look are overall in agreement with results obtained with conventional susceptibility tests (62)(63)(64)(65)(66)(67)(68).…”

Section: Alternative Methods For Susceptibility Testingsupporting

confidence: 73%

Biofilm antimicrobial susceptibility testing: where are we and where could we be going?

Coenye

2023

Clin Microbiol Rev

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SUMMARY Our knowledge about the fundamental aspects of biofilm biology, including the mechanisms behind the reduced antimicrobial susceptibility of biofilms, has increased drastically over the last decades. However, this knowledge has so far not been translated into major changes in clinical practice. While the biofilm concept is increasingly on the radar of clinical microbiologists, physicians, and healthcare professionals in general, the standardized tools to study biofilms in the clinical microbiology laboratory are still lacking; one area in which this is particularly obvious is that of antimicrobial susceptibility testing (AST). It is generally accepted that the biofilm lifestyle has a tremendous impact on antibiotic susceptibility, yet AST is typically still carried out with planktonic cells. On top of that, the microenvironment at the site of infection is an important driver for microbial physiology and hence susceptibility; but this is poorly reflected in current AST methods. The goal of this review is to provide an overview of the state of the art concerning biofilm AST and highlight the knowledge gaps in this area. Subsequently, potential ways to improve biofilm-based AST will be discussed. Finally, bottlenecks currently preventing the use of biofilm AST in clinical practice, as well as the steps needed to get past these bottlenecks, will be discussed.

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Section: Alternative Methods For Susceptibility Testingsupporting

confidence: 73%

Biofilm antimicrobial susceptibility testing: where are we and where could we be going?

Coenye

2023

Clin Microbiol Rev

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“…Baldoni et al showed the differences in the heat flow curves generated by MRSA and MSSA strains (12). Also, Grütter et al investigated detection and drug susceptibility testing in two different strains of Neisseria gonorrhoeae through isothermal microcalorimetry, and highlighted the differences found in the thermograms between strains of the same organism (13). These results suggest that characterization of the thermogram profiles could facilitate the identification of the microorganism causing an infection, including its level of susceptibility to antibiotic treatment.…”

Section: Introductionmentioning

confidence: 99%

Towards faster identification of MRSA and MSSA: analysis of calorimetric curve parameters from large hospital bacterial collections

Zunk-Parras,

Trampuz,

Arias-Sánchez

2023

Preprint

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There is a need to develop faster yet precise diagnostic tools for the identification of infectious agents and their levels of antimicrobial susceptibility. One such tool is calorimetry, as previous studies show that bacteria display unique signatures of calorimetric/metabolic activity that such signatures can be used for pathogen identification under controlled laboratory conditions. However, the level of variation of these unique signatures remains largely unexplored. Here, we use real-time isothermal microcalorimetry to disentangle the inter- and intra-species metabolic differences betweenS. aureusandS. epidermidis. We worked with a large collection of bacterial isolates obtained from patients with prosthetic joint infections as well as reference laboratory strains. We analyzed their calorimetric profiles and decomposed the curve parameters derived from them. As a result, we identified six calorimetric parameters that are useful for identification at the intra-species level, with a particular focus on MRSA. Furthermore, we found important differences between the thermograms of ATCC laboratory strains when compared against model thermograms generated from all our clinical isolates. These results indicate that accounting for metabolic variability can impact diagnosis precision. Finally, we discuss our findings and suggest ways to optimize calorimetric diagnostics and treatment approaches.

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“…This Special Issue collects examples of quantitative IMC analyses which address microbial growth in various contexts. Grütter et al demonstrate that IMC can be used as a diagnostic tool in urethritis caused by Neisseria gonorrhoea , a pathogen which is difficult to culture for diagnostic purposes [ 7 ]. With IMC, the microorganism is detectable at concentrations as low as 1 CFU·mL −1 , revealing both an organism-specific signature in the heat flow curves and its drug susceptibility.…”

mentioning

confidence: 99%

Special Issue “Advances in Monitoring Metabolic Activities of Microorganisms by Calorimetry”

2023

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Detection and Drug Susceptibility Testing of Neisseria gonorrhoeae Using Isothermal Microcalorimetry

Cited by 5 publications

References 51 publications

Biofilm antimicrobial susceptibility testing: where are we and where could we be going?

Biofilm antimicrobial susceptibility testing: where are we and where could we be going?

Towards faster identification of MRSA and MSSA: analysis of calorimetric curve parameters from large hospital bacterial collections

Special Issue “Advances in Monitoring Metabolic Activities of Microorganisms by Calorimetry”

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