Detection and evaluation of DNA methylation markers found at SCGN and KLF14 loci to estimate human age

Alghanim, Hussain; Antunes, Joana; Silva, Deborah Soares Bispo Santos; Alho, Clarice Sampaio; Balamurugan, Kuppareddi; McCord, Bruce

doi:10.1016/j.fsigen.2017.07.011

Cited by 45 publications

(34 citation statements)

References 44 publications

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“…[31][32][33][34] Several studies reported that, among the ageing-related CpG sites, KLF14 promoter region was associated with chronological age. [45][46][47] Furthermore, with ageing and obesity, the level of DNA methylation in KLF14 promoter was increased significantly in several organs in mice, which led to downregulation of KLF14. 47 Moreover, the KLF14 gene was under a hypermethylation state in familial Alzheimer's disease, which regulated cell death signalling.…”

Section: F I G U R Ementioning

confidence: 99%

“…[45][46][47] Furthermore, with ageing and obesity, the level of DNA methylation in KLF14 promoter was increased significantly in several organs in mice, which led to downregulation of KLF14. 47 Moreover, the KLF14 gene was under a hypermethylation state in familial Alzheimer's disease, which regulated cell death signalling. 48 However, in this study, we found that inhibition of DNMTs using 5-azadC did not significantly increased KLF14 expression, however, targeting EZH2 by EPZ-6438 or LV-shRNA dramatically reactivated KLF14 expression in HSCs.…”

Section: F I G U R Ementioning

confidence: 99%

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EZH2‐mediated inhibition of KLF14 expression promotes HSCs activation and liver fibrosis by downregulating PPARγ

Liu

Wang

et al. 2021

Cell Proliferation

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Section: F I G U R Ementioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

EZH2‐mediated inhibition of KLF14 expression promotes HSCs activation and liver fibrosis by downregulating PPARγ

Liu

Wang

et al. 2021

Cell Proliferation

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“…One approach is to identify a large set of CpG sites that show linear correlation with aging based on EWASs [18,19]. Other studies have examined the use of more specific subsets of age-related CpG sites ranging from 1 to 5 CpGs [20][21][22]. In our lab, similar approach was taken by identifying small set of CpG sites located at SCGN and KLF14 [22].…”

Section: Phenotyping and Age Determinationmentioning

confidence: 99%

Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

McCord

Gauthier

Alghanim

et al. 2019

Forensic Science International: Genetics Supplement Series

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In this paper, four different applications are presented in forensic epigenetics using bisulfite modified PCR, HRM, and pyrosequencing. The first application involves the development of a multiplex amplification capable of simultaneous analysis of 4 different body fluid/cell types-blood, saliva, sperm, and vaginal epithelia. We have also developed a method for the detection of sperm high-resolution melt analysis (HRM). Two other applications involve a determination of suspect age and a biomarker for determining smoking status.

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“…Universal age-related markers would have significant benefits in terms of developing models to predict age across multiple tissues, but the highest age-prediction accuracy has been seen in tissue-specific models [102,104,[121][122][123]. These have mainly focused on whole blood [124][125][126][127][128], with some studies on other tissues such as saliva and semen [115,[129][130][131][132]. A number of MPS-based targeted methylation assays for age prediction have also now been developed, overcoming the multiplexing limitations of previous technologies and permitting analysis of multiple CpG sites in a single highly sensitive assay [104,127,133,134].…”

Section: Epigenetics and Dna Methylation Analysismentioning

confidence: 99%

Developments in forensic DNA analysis

Haddrill

2021

Emerging Topics in Life Sciences

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The analysis of DNA from biological evidence recovered in the course of criminal investigations can provide very powerful evidence when a recovered profile matches one found on a DNA database or generated from a suspect. However, when no profile match is found, when the amount of DNA in a sample is too low, or the DNA too degraded to be analysed, traditional STR profiling may be of limited value. The rapidly expanding field of forensic genetics has introduced various novel methodologies that enable the analysis of challenging forensic samples, and that can generate intelligence about the donor of a biological sample. This article reviews some of the most important recent advances in the field, including the application of massively parallel sequencing to the analysis of STRs and other marker types, advancements in DNA mixture interpretation, particularly the use of probabilistic genotyping methods, the profiling of different RNA types for the identification of body fluids, the interrogation of SNP markers for predicting forensically relevant phenotypes, epigenetics and the analysis of DNA methylation to determine tissue type and estimate age, and the emerging field of forensic genetic genealogy. A key challenge will be for researchers to consider carefully how these innovations can be implemented into forensic practice to ensure their potential benefits are maximised.

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Detection and evaluation of DNA methylation markers found at SCGN and KLF14 loci to estimate human age

Cited by 45 publications

References 44 publications

EZH2‐mediated inhibition of KLF14 expression promotes HSCs activation and liver fibrosis by downregulating PPARγ

EZH2‐mediated inhibition of KLF14 expression promotes HSCs activation and liver fibrosis by downregulating PPARγ

Applications of epigenetic methylation in body fluid identification, age determination and phenotyping

Developments in forensic DNA analysis

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